TOPICS 2018
-
Dr. Masato Hosokawa (Dementia Research Project) was awarded the 2nd Serika fund for ALS research.
-
Tomoyuki Miyashita published a paper on ”Long-term memory encoding engram neurons are established by the transcriptional cycling” in Cell Reports.
-
Notice with respect to a license agreement with uniQure and the development of gene therapy encoding a new enzyme, “modified NAGA”, for Fabry disease
-
Masaki Oba (Diabetic Neuropathy Project) had won the Junior Investigator Poster Award of the 41st annual meeting of the Japan Neuroscience Society and the Young Investigator Award of the 28th annual meeting of Japanese Society of Pathophysiology
-
Shinobu Hirai, Haruo Okado (Neural Development Project) with Koji Hotta（keio University） published a paper on “Developmental roles and evolutionary significance of AMPA-type glutamate receptors” in BioEssays.
-
Takeru Honda, PhD, (Motor Disorders Project) published a paper on “Tandem Internal Models Execute Motor Learning in the Cerebellum” in PNAS.
-
Chiaki Ohtaka-Maruyama, PhD, (Neural Network Project) published a paper on “Synaptic communication controls neuronal migration” in Science.
-
Yuichiro Miyaoka, PhD, (Regenerative Medicine Project) published a paper on “New way to enhance precise genome editing via HDR induced by CRISPR/Cas9” in Nucleic Acids Research.
-
Dr.Atsushi Nishida, (Mental Health Promotion Project) published a paper in Journal of Psychiatry Research.
-
Nature Index 2018 Japan announced the tables which show Japan’s leading institutions, our institute ranked first in "life science" field
-
Dr. Miharu Nakanishi of Mental Health Promotion Project and Mental Health and Nursing Research Team had made a presentation in the Japan-UK Dementia Conference.
-
Hikaru Tsuchiya, PhD, published a paper on “A novel versatile method for assessing chain length of substrate-attached polyubiquitin chains” in Nature Communications.
-
Fumiaki Ohtake, Ph.D. published a paper on “K63 ubiquitylation triggers proteasomal degradation by seeding branched ubiquitin chains” in PNAS.
-
Dr. Koji Yamano, published a paper on “Elucidation of a new mechanism in which damaged mitochondria are degraded through autophagy machinery” in Elife.
-
Masanari Itokawa published a paper on “Pyridoxamine: A novel treatment for schizophrenia with enhanced carbonyl stress.” in Psychiatry and clinical neuroscience.
HOME ＞ Topics2018 ＞ 19 April 2018
17 April 2018
Yuichiro Miyaoka, PhD, (Regenerative Medicine Project) published a paper on “New way to enhance precise genome editing via HDR induced by CRISPR/Cas9.” in Nucleic Acids Research.
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Background and Aim
CRISPR/Cas9 allows for genetic manipulations in basically all species, which has a great potential in therapeutic and agricultural applications. CRISPR/Cas9 specifically cuts target genomic DNA sequences to activate the two DNA repair pathways, NHEJ and HDR. NHEJ induces DNA insertions and deletions, whereas HDR precisely repairs the genome via recombination between the genomic DNA and template DNA. However, CRISPR/Cas9 always generates NHEJ along with HDR. Therefore, we investigated how the balance between HDR and NHEJ is determined by CRISPR/Cas9.
- ＜Title of the paper＞
- Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
- ＜Journal＞
- Nucleic Acids Research. Volume 46, Issue 9, 18 May 2018, Pages 4677–4688,
https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gky264/4972875
Published: 17 April 2018
Methods and Results
We utilized our original methods to detect single nucleotide substitutions and knock-ins of a multi-kb gene fragment mediated by HDR, and found that Cas9 variants and modified gRNAs with altered interactions with the genomic DNA exhibited higher HDR/NHEJ ratios. Our results indicate that alterations in the interactions between CRISPR/Cas9 and the genomic DNA can lead to more precise and efficient genome editing mediated by HDR.
Regenerative Medicine Project ▶
