発表論文

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Original Papers (2000 – 2023)

-2023-

  1. Hao-Wen Hsiao, Chi-Chun Yang, and *Hisao Masai (2023) “Claspin-dependent and -independent Chk1 activation by a panel of biological stresses.” Biomolecules,13(1): 125.
  2. Kanoh, Y., Matsumoto, S., Ueno, M., Hayano, M., Kudo, M., and *Masai, H. (2023) “Aberrant association of chromatin with nuclear periphery induced by Rif1 leads to mitotic defect and cell death.” Life Science Alliance 6(4):e202201603.
  3. Hori, K., Yamazaki, S., Maruyama, C., Iguchi, T. and *Masai. H. (2023) “Cdc7 kinase is required for postnatal brain development.” Genes to Cells, in press
  4. Wendi Sun*, Kian Leong Lee*, Lorenz Poellinger, Hisao Masai and *Hiroyuki Kato (2023) “Catalytic domain-dependent and -independent transcriptional activities of the tumor suppressor histone H3K27 demethylase UTX/KDM6A in specific cancer types.” Epigenetics 18, 2222245.
  5. Tamas Nemeth, Naoko Yoshizawa-Sugata, Agnes Pallier, Youichi Tajima, Éva Tóth,
    *Hisao Masai, and *Yoko Yamakoshi(2023) “Water-Soluble Gd(III)-Porphyrin Complexes Capable of Both Photosensitization and Relaxation Enhancement.” Chemical & Biomedical Imaging 1(2):157-167.
  6. *Yoshizawa-Sugata N, Masai H. (2023)Histone Modification Analysis of Low-Mappability Regions. Methods Mol. Biol. 2023;2519:163-185. doi: 10.1007/978-1-0716-2433-3_18. PMID: 36066721.

-2022-

  1. Tajima, Y., Shibasaki, F. and *Masai, H. (2022) “Cell fusion upregulates PD-L1 expression and promotes tumor formation.” Oncogene revised manuscript submitted (bioRxiv 2022.06.14.496068)
  2. Nakamura H, Sekine H, Kato H, Masai H, Gradin K, Poellinger L. Hypoxia-inducible factor-1α and poly [ADP ribose] polymerase 1 cooperatively regulate Notch3 expression under hypoxia via a noncanonical mechanism. J Biol Chem. 2022 Jul;298(7):102137. doi: 10.1016/j.jbc.2022.102137. Epub 2022 Jun 14. PMID: 35714766; PMCID: PMC9287808.

-2021-

  1. *Yoshizawa-Sugata, Y., Yamazaki, S., Mita-Yoshida, K., Ono, T., Nishito, Y., and *Masai, H. (2021) “Loss of full-length Rif1 protein in 2-cell embryos is associated with zygotic transcriptional activation.” J. Biol. Chem. 297(6):101367
  2. Kitajima S, Sun W, Lee KL, Ho JC, Oyadomari S, Okamoto T, Masai H, Poellinger L, *Kato H. (2021) A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition. Sci Rep. 11(1):4538.
  3. Nakamura K, Sakai S, Tsuyama J, Nakamura A, Otani K, Kurabayashi K, Yogiashi Y, Masai H, *Shichita T. Extracellular DJ-1 induces sterile inflammation in the ischemic brain. PLoS Biol. 2021 May 20;19(5):e3000939. doi: 10.1371/journal.pbio.3000939. PMID: 34014921; PMCID: PMC8136727.
  4. *Irie, T., Asami, T., Sawa, A., Uno, Y., Taniyama, C., Funakoshi, Y., Masai, H., Sawa, M. (2021) “Discovery of AS-0141, a Potent and Selective Inhibitor of CDC7 Kinase for the Treatment of Solid Cancers.” J.  Med. Chem. 64(19):14153-14164. doi: 10.1021/acs.jmedchem.1c01319.

-2020-

  1. *Masai, H, Kanoh, Y, Kakusho, N, Fukatsu, R (2020) “Detection of cellular G-quadruplex by using a loop structure as a structural determinant.” Biochemical and Biophysical Research Communications, 531:75-83.
  2. *Kato H, Asamitsu K, Sun W, Kitajima S, Yoshizawa-Sugata N, Okamoto T, Masai H, Poellinger L. (2020) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization. Oncogene. doi: 10.1038/s41388-020-1218-3. [Epub ahead of print] PubMed PMID: 32071397.
  3. Fracassi A, Cao J, Yoshizawa-Sugata N, Toth E, Archer C, Groninger O, Ricciotti E, S-Y Tang, Handschin S, Bourgeois J-P, Ray A, Liosi K, Oriana S, Stark W, Masai H, Zhou R, Yamakoshi Y (2020) “LDL-mimetic lipid nanoparticles prepared by surface KAT ligation for in vivo MRI of atherosclerosis” Chem Sci in press
  4. Kitajima S, Sun W, Lee KL, Ho JC, Oyadomari S, Okamoto T, Masai H, Poellinger L, Kato H. (2021) A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition. Sci Rep. 11(1):4538.

-2019-

  1. Yang, C-C., Kato, H., Shindo, M. and *Masai. H. (2019)”Cdc7 activates replication checkpoint by phosphorylating the Chk1 binding domain of Claspin in human cells.” E-life, 8. pii: e50796.
  2. *Masai, H., Fukatsu, R., Kakusho, N., Kanoh, Y., Moriyama, K., Ma, Y., Iida, K., Nagasawa, K. (2019) “Rif1 promotes self-association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities.” Sci. Rep., 9(1), 8618.
  3. Kobayashi, S., Fukatsu, R., Kanoh, Y., Kakusho, N., Matsumoto, S., Chaen, S. and *Masai, H. (2019) “Both a unique motif at the C terminus and N-terminal HEAT repeat contribute to G4 binding and origin regulation by Rif1 protein.” Mol Cell. Biol. 39, pii: e00364-18.(cover figure)
  4. *Masai, H., Fukatsu, R., Kakusho, N., Kanoh, Y., Moriyama, K., Ma, Y., Iida, K., Nagasawa, K. (2019) “Rif1 promotes self-association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities.” Sci. Rep.,  9(1):18622.

-2018-

  1. Moriyama K, Yoshizawa-Sugata N, and *Masai H  (2018) Oligomer formation and G-quadruplex-binding by purified murine Rif1 protein, a key organizer of higher-order chromatin architecture. J Biol Chem.  pii: jbc.RA117.000446. doi: 10.1074/jbc.RA117.000446. [Epub ahead of print] PubMed PMID: 29348174.
  2. Iguchi, T., Miyauchi, E., Watanabe, S., Masai, H. and *S. Miyatake (2018) “A BTB-ZF protein, ZNF131, is required for early B cell development.” Biochemical and Biophysical Research Communications, 501, 70-575.
  3. *Masai, H., Kakusho, N., Fukatsu, R., Ma, Y., Iida, K., Kanoh, Y. and Nagasawa, K. (2018) “Molecular architecture of G-quadruplex structures generated on duplex Rif1 binding.” J Biol Chem. 293, 17033-17049.

-2017-

  1. *You, Z. and Masai, H. (2017) “Potent DNA strand annealing activity associated with mouse Mcm2~7 heterohexamer complex.” Nucleic Acids Res. 45, :6494-6506. [Pubmed]
  2. Irie T, Asami T, Sawa A, Uno Y, Hanada M, Taniyama C, Funakoshi Y, Masai H, and *Sawa M. (2017) Discovery of novel furanone derivatives as potent Cdc7 kinase inhibitors. Eur J Med Chem. 130, 406-418. doi: 10.1016/j.ejmech.2017.02.030. Epub 2017 Feb 17. PubMed PMID: 28279847.
  3. Matsumoto, S., Kanoh, Y., Shimmoto, M., Hayano, M., Ueda, K., Fukatsu, R., Kakusho, N., and *Masai, H. (2017) “Checkpoint-independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 kinase.” Mol. Cell. Biol.  Mar 17;37(7). pii: e00355-16. doi: 10.1128/MCB.00355-16. Print 2017 Apr 1. PMID: 28069740
  4.  Toteva, T., Mason, B., Kanoh, Y. Brøgger, P. Green, D., Verhein-Hansen, J. Masai, H. and *Thon, G. (2017) “Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast.” Proc. Natl. Acad. Sci. USA. 114, 1093-1098.

-2016-

  1. Yang, C-C., Suzuki, M., Yamakawa, S., Uno, S., Ishii, A., Yamazaki, S., Fukatsu, R., Fujisawa, R., Sakimura, K., Tsurimoto, T., and *Masai, H. (2016) “Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells.” Nature Communications 7:12135 doi: 10.1038/ncomms12135.
  2. *Zhiying You, Koji L. Ode, Haruhiko Takisawa, and Hisao Masai (2015) “Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex.” Cell Cycle 15:1213-26.
  3. Tanaka T, Nishito Y, *Masai H. (2016) “Fork restart protein, PriA, binds around oriC after depletion of nucleotide precursors: Replication fork arrest near the replication origin.” Biochem. Biophys. Res. Commun. 470, 546-551.
  4.  Tanaka H, Muto A, Shima H, Katoh Y, Sax N, Tajima S, Brydun A, Ikura T, Yoshizawa N, Masai H, Hoshikawa Y, Noda T, Nio M, Ochiai K, *Igarashi K. (2016) “Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3.” J. Biol. Chem. 291, 6316-6330.
  5. *Nonaka T, Suzuki G, Tanaka Y, Kametani F, Hirai S, Okado H, Miyashita T, Saitoe M, Akiyama H, Masai H, Hasegawa M. (2016) “Phosphorylation of TAR DNA-binding Protein of 43 kDa (TDP-43) by Truncated Casein Kinase 1δ Triggers Mislocalization and Accumulation of TDP-43.” J. Biol. Chem. 291, 5473-5483.
  6. Kozel, C., Thompson, B., Hustak, S., Moore, C., Nakashima, A., Singh, C.R., Reid, M., Cox, C., Papadopoulos, E., Luna, R.E., Anderson, A., Tagami, H., Hiraishi, H., Slone, E.A., Yoshino, K.I., Asano, M., Gillaspie, S., Nietfeld, J., Perchellet, J.P., Rothenburg, S., Masai, H., Wagner, G., Beeser, A., Kikkawa, U., Fleming, S.D., and *Asano, K. (2016) “Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.” Nucleic Acids Res. 44, 8704-8713.

-2015-

  1. Yutaka Kanoh, Seiji Matsumoto, Rino Fukatsu, Naoko Kakusho, Nobuaki Kono, Claire Renard-Guillet, Koji Masuda, Keisuke Iida, Kazuo Nagasawa, Katsuhiko Shirahige, and *Hisao Masai (2015) Rif1 binds to G-quadruplex and suppresses replication over a long distances. Nature Structural & Molecular Biology Article in press [PubMed]
  2. Zech J, Godfrey EL, Masai H, Hartsuiker E, Dalgaard JZ. (2015) “The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.” PLoS One. 10:e0132595. [PubMed]
  3. Iguchi T, Aoki K, Ikawa T, Taoka M, Taya C, Yoshitani H, Toma-Hirano M, Koiwai O, Isobe T, Kawamoto H, Masai H, Miyatake S. (2015) “BTB-ZF Protein Znf131 Regulates Cell Growth of Developing and Mature T Cells.” J Immunol.  195, 982-993. [PubMed]
  4. *Kotaro Koiwai, Takashi Kubota, Nobuhisa Watanabe, Katsutoshi Hori, Osamu Koiwai, and *Hisao Masai (2015) “Definition of the transcription factor TdIF1 consensus binding sequence through genome-wide mapping of its binding sites.” Genes to Cells 20, 242-254. [PubMed]
  5. Jeffery, D.C., Kakusho, N., You, Z., Gharib, M., Wyse, B., Drury, E., Weinreich, M., Thibault, P., Verreault, A., Masai, H., and *Yankulov, K. (2015) “CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor with chromatin.” Cell Cycle 14, 74-85. [PubMed]

-2014-

  1. Bellelli, R., Castellone, M.D., Guida, T., Limongello, R., Dathan, N.A., Merolla, F., Cirafici, A.M., Affuso, A., Masai, H., Costanzo, V., Grieco, D., Fusco, A., Santoro, M., and *Carlomagno, F. (2014) “NCOA4 Transcriptional Coactivator Inhibits Activation of DNA Replication Origins.” Mol. Cell 55, 123-137. [PubMed]

  2. Yamada, M., Masai, H., and *Bartek, J. (2014) “Regulation and roles of Cdc7 kinase under replication stress.” Cell Cycle 13, 1859-1866. [PubMed]

-2013-

  1. Yamada, M., Watanabe, K., Mistrik, M., Mailand, N., Lee, M-H., Masai, H., Lukas, J., and *Bartek, B. (2013) “ATR-Chk1-APC/CCdh1-dependent stabilization of Cdc7-ASK(Dbf4) kinase complex is required for DNA damage bypass under replication stress.” Genes and Development 27:2459-2472. [PubMed]

  2.  You, Z., De Falco, S., Pisani, F.M. and *Masai, H. (2012) “MCM helicase interacts with primase and stimulates its priming activity.” PLoS One 8, e72408 [PubMed]

  3.  Aria, V., De Felice, M., Di Perna, R., Uno, S., Masai, H., Syvaoja, J.E., van Loon, B., Hubscher, U., and *Pisani, F.M. (2013) “The Human Tim/Tipin Complex Directly Interacts with DNA Polymerase {epsilon} and Stimulates its Synthetic Activity.” J. Biol. Chem. 288, 12742-12752. [PubMed]

  4.  Jeffery, D.C., Wyse, B.A., Rehman, M.A., Brown, G.W., You, Z., Oshidari, R., Masai, H., and *Yankulov, K.Y. (2013) “Analysis of epigenetic stability and conversions in Saccharomyces cerevisiae reveals a novel role of CAF-I in position-effect variegation.” Nucleic Acids Res. 41, 8475-8488. [PubMed]

  5.  Tanikawa, M., Wada-Hiraike, O., Yoshizawa-Sugata, N., Shirane A, Hirano M, Hiraike H, Miyamoto, Y., Sone, K., Ikeda, Y., Kashiyama, T., Oda K, Kawana K, Katakura Y, Yano T, Masai, H., Roy AL, Osuga, Y., and *Fujii, T. (2013) “Role of multifunctional transcription factor TFII-I and putative tumour suppressor DBC1 in cell cycle and DNA double strand damage repair.” Br. J. Cancer. 109, 3042-3048. [PubMed]

-2012-

  1. Hayano, M., Kanoh, Y., Matsumoto, S., Shrahige, K., and *Masai, H.  (2012) “Rif1 is a global regulator of timing of replication origin firing in fission yeast.” Genes and Development, 26,137-150. (“Exceptional” evaluation in F1000; Highlighted in A-IMBN Research)
  2. Yamazaki, S., Ishii, A., Kanoh, Y., Oda, M., Nishito, Y., and *Masai, H. (2012) “Rif1 protein is a key regulator of the genome-wide DNA replication timing in human cells.” EMBO J. 31, 3167-3177. (Highlighted in Commentary; highlighted in A-IMBN Research)
  3. Barkley, L.R., Palle, K., Durando, M., Day, T.A., Gurkar, A., Kakusho, N., Li, J., Masai, H., and *Vaziri, C. (2012) “c-Jun N-terminal Kinase (JNK)-Mediated Rad18 Phosphorylation Facilitates Polη Recruitment to Stalled Replication Forks.” Mol. Biol. Cell. 23, 1943-1954.
  4. Moriyama, K., Yoshizawa-Sugata, N., Obuse, C., Tsurimoto, T., and *Masai, H.  (2012) “EBNA1-dependent recruitment of Orc on OriP of Epstein-Barr virus with purified proteins: Stimulation by Cdc6 through Its direct interaction with EBNA1.” J. Biol. Chem. 287, 23977-23994.
  5. Ito, S., Ishii, A., Kakusho, N., Taniyama, C., Yamazaki, S., Sakaue-Sawano, A., Miyawaki, A., and *Masai, H. (2012) “Mechanism of cancer cell death induced by depletion of an essential replication regulator.” PLoS One, 7, e36372. (Highlighted in A-IMBN Research)
  6. Uno, S., You, Z., and *Masai, H. (2012) “Purification of replication factors using insect and mammalian cell expression systems.” Methods, 57, 214-221.
  7. Oda, M., Kanoh, Y., Watanabe, Y., and *Masai, H. (2012) “Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1.” PLoS One 7, e42375.
  8. Miyoshi, T., Kugou, K., Yamada, S., Ito, M., Furuichi, M., Oda, A., Hirota, K. and Masai, H. and *Ohta, K. (2012) “A central coupler for recombination initiation linking chromosome architecture to S-phase checkpoint.” Mol. Cell 47, 722-733.
  9. Suzuki, T., Tsuzuku, J., Hayashi, A., Shiomi, Y., Iwanari, H., Mochizuki, Y., Hamakubo, T., Kodama, T., Nishitani, H., Masai, H. and *Yamamoto, T. (2012) “Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob.” J. Biol. Chem. 287, 40256-40265. (Highlighted in A-IMBN Research)

-2011-

  1. Kitamura, R., Fukatsu, R., Kakusho, N., Cho, Y-S., Taniyama, C., Yamazaki, S., Toh, G-T., Yanagi, K., Arai, N., Chang, H-J., and *Masai, H. (2011) “Molecular mechanism of activation of human Cdc7 kinase: Bipartite interaction with Dbf4/ASK stimulates ATP binding and substrate recognition.” J. Biol. Chem. 286, 23031-23043.
  2. Hayano, M., Kanoh, Y., Matsumoto, S., Kakusho, N. and *Masai, H. (2011) “Pre-firing binding of Mrc1 defines the early-firing origins which are selectively hyper-activated upon loss of fork stabilizing factors in fission yeast.” Mol. Cell. Biol. 31, 2380-2389. (“Recommended” evaluation in F1000)
  3. Uno, S and *Masai, H. (2011) “Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells: DNA binding activity and novel protein interactions.” Genes to Cells, 16, 842-856.
  4. Matsumoto, S., Hayano, M., Kanoh. Y. and *Masai, H. (2011) “Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.” J. Cell Biol. 195, 387-401. (“Must Read” evaluation in F1000)

-2010-

  1. Tanaka, T., Yokoyama, M., Matsumoto, S., Fukatsu, R., You, Z. and *Masai, H. (2010) “Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1.” J. Biol. Chem. 285, 39609-39622.
  2. Kundu,L.R., Kumata, Y, Kakusho, N., Watanabe, S., Furukohri, A., Waga, S., Sekia, M., Masai, H., Enomoto, T., and *Tada, S. (2010) “Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex.” Nucleic Acid Res. 38, 5409-5418.
  3. Takeishi, Y., Ohashi, E., Ogawa, K, Masai, H., Obuse, C., and *Tsurimoto, T. (2010) “Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1.” Genes Cells 15, 761-771.
  4. Furuya, K., Miyabe, I., Tsutsui, Y., Paderi, F., Kakusho, N., Masai, H., Niki, H., and *Carr, A. M. (2010) “DDK phosphorylates checkpoint clamp Rad9 and promotes its release from damaged Chromatin.” Mol. Cell 40, 606-618.
  5. Day, T.A., Palle, K., Barkley, L.R., Kakusho, N., Zou, Y., Tateishi, S., Verreault, A., Masai, H., and *Vaziri, C. (2010) “Cdc7-Mediated Rad18 Phosphorylation Directs the Accumulation of DNA Polymerase η at Sites of Stalled Replication.” J. Cell Biol. 191, 953-966.
  6. Matsumoto, S., Shimmoto, M., Kakusho, N., Yokoyama, M., Russell, P., and *Masai, H. (2010) “Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.” Cell Cycle 9, 4627-4637.

-2009-

  1. Shimmoto, S., Matsumoto, S., Hayano, M., Yokoyama, M., Noguchi, E., Russell, P. and *Masai, H. (2009) “Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast.” Genes to Cells 14, 669-682.
  2. Tanaka, H., Kubota, Y., Tsujimura, T., Kumano, M., Masai, H. and *Takisawa, H. (2009) “Replisome progression complex links DNA replication to sister chromatid cohesion in Xenopus egg extracts.” Genes to Cells 14, 949-963.
  3. Yoshizawa-Sugata, N. and *Masai, H. (2009) “Roles of human AND-1 in chromosome transactions in S phase.” J. Biol. Chem. 284, 20718-20728.

-2008-

  1. Kim, J-M., Kakusho, N., Yamada, M., Kanoh, Y., Takemoto, N., and *Masai, H. (2008) “Cdc7 kinase is required for Claspin phosphorylation in DNA replication checkpoint.” Oncogene 27, 3475-3482.
  2. Sasanuma, H., Hirota, K., Fukuda, T., Kakusho, N., Kugou, N., Kawasaki, Y., Shibata, T., Masai, H., and *Ohta, K. (2008) “Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination.” Genes & Dev. 22, 398-410.
  3. Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Kashiwagi, S., Fukami, K., Imamura, T., Ogawa, M., Masai, H. and *Miyawaki, A. (2008) “Spatio-temporal dynamics of multicellular cell cycle progression.” Cell 132, 487-498. (Featured on the cover of the issue; “Exceptional” evaluation in F1000)
  4. Kakusho, N., Taniyama, C., and *Masai, H. (2008) “Identification of stimulators and inhibitors of CDC7 kinase in vitro.” J. Biol. Chem. 283, 19211-19218.
  5. You Z. and Masai H. (2008) “Cdt1 forms a complex with MCM and activates its helicase activity.” J. Biol. Chem. 283, 24469-24477.

-2007-

  1. Yoshizawa-Sugata, N. and *Masai, H. (2007) “Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint.” J. Biol. Chem. 282, 2729-2740
  2. Tanaka, T., Mizukoshi, T., Sasaki, K., Kohda, D. and Masai, H. (2007) “Escherichia coli PriA protein: Two modes of DNA binding and activation of ATP hydrolysis.” J. Biol. Chem. 282, 19917-19927.
  3. Sasaki, K., Ose, T., Okamoto, N., Maenaka, K., Tanaka, T., Masai, H., Saito, M., Shirai, T., and D. Kohda (2007) “Structural basis of the 3′-end recognition of a leading strand in stalled DNA replication forks by PriA. EMBO J. 26,19917-19927

-2006-

  1. Ogino, K. and Masai, H. (2006) “Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication: Mei4-dependent transcription as a potential target of meiotic checkpoint.” J. Biol. Chem., 281,1338-1344.
  2. Tanaka, T. and Masai, H. (2006) “Stabilization of a stalled replication fork by concerted actions of two helicases.” J. Biol. Chem. 281, 3484-3493.
  3. Sasaki, K., Ose, T., Tanaka, T., Mizukoshi, T., Ishigaki, T., Maenaka,K., Masai, H., and *Kohda, D. (2006) “Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli.” Biochim. Biophys. Acta., 1764, 157-160.
  4. Kitamura, R., Sekimoto, T., Ito, S., Harada, S., Yamagata, H., Masai, H., Yoneda, H., and *Yanagi, K. (2006) “Nuclear import of Epstein-Barr Virus Nuclear Antigen 1 mediated by NPI-1 (Importin a5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential.” J. Virol., 80, 1979-1991.
  5. Ogino, K., Hirota, K., Matsumoto, S., Takeda, T., Ohta, K., Arai, K., and *Masai, H. (2006) “Hsk1 kinase is required for induction of meiotic double-stranded DNA breaks without involving checkpoint kinases in fission yeast.” Proc. Natl. Acad. Sci. USA, 103, 8131-8136.
  6. Hayashida, T., Oda , M., Ohsawa, K., Yamaguchi, A., Giacca, M., Locksley, R.M., Masai H.*, and Miyatake, S.* (2006) “Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved non-coding sequence.” (*cocommunicating authors) J. Immunol., 176, 5446-5454.
  7. *Masai, H., Taniyama, C., Ogino, K., Matsui, E., Kakusho, N., Matsumoto, M., Kim, J-M., Ishii, A., Tanaka, T., Kobayashi, T., Tamai, K., Ohtani, K., and Arai, K. (2006) “Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin.” J. Biol. Chem., 281, 39249-32961. (This paper was selected as “JBC paper of the week” and was featured in the cover of December 22 issue of JBC.)

-2005-

  1. Fujii-Yamamoto, H., Kim, J-M., Arai, K. and *Masai, H. (2005) “Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells.” J. Biol. Chem. 280, 12976-12987.
  2. Yoshizawa-Sugata, N., Ishii, A., Taniyama, C., Matsui, E., Arai, K., and *Masai, H. (2005) “A second human Dbf4/ASK-related protein, Drf1/ASKL1 is required for efficient completion of S and M phases.” J. Biol. Chem. 280, 13062-13070.
  3. Yamashita, N., Kim, J-M., Koiwai, O., Arai, K. and *Masai, H. (2005) “Functional analyses of mouse ASK, an activator subunit for Cdc7 kinase, using conditional ASK knockout ES cells.” Genes to Cells 50, 551-563.
  4. You, Z. and *Masai, H. (2005) “DNA binding and helicase actions of mouse Mcm4/6/7 helicase.” Nucl. Acids Res. 33, 3033-3047.
  5. Todorovic, V., Giadrossi, S., Pelizon, C., Masai, H. and *Giacca, M. (2005) “Novel human origins of DNA replication selected from a library of nascent DNA.” Mol. Cell 19, 567-575.
  6. Matsumoto, S., Ogino, K., Noguchi, E., Russell, P. and *Masai, H. (2005)  “Hsk1-Dfp1/Him1, the Cdc7-Dbf4 Kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex.” J. Biol. Chem., 280, 42536-42542.

-2004-

  1. Schnepp, R.W., Hou, Z., Wang, H., Petersen, C., Silva, A, Masai, H. and *Hua X. (2004) “Functional interaction between tumor suppressor menin and activator of S-phase kinase.” Cancer Res. 64, 6791-6796.
  2. Kurita, M., Suzuki, H., Masai, H., Mizumoto, K., Ogata, E., Nishimoto, I., Aiso, S., and *Matsuoka, M. (2004) “Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest.” Biochem. Biophys. Res. Commun. 32, 554-561.

-2003-

  1. Tanaka, T., Taniyama, C., Arai, K., and *Masai, H. (2003) “ATPase/helicase motif mutants of Escherichia coli PriA protein essential for recombination-dependent DNA replication.” Genes to Cells, 8, 251-261.
  2. Sato, N., Sato, M., Nakayama, M., Saitoh, R., Arai, K., and *Masai, H. (2003) “Cell cycle regulation of chromatin binding and nuclear localization of Cdc7-ASK kinase complex.” Genes to Cells, 8, 451-463.
  3. Kim, J.M., Takemoto, N., Arai K. and *Masai, H. (2003) “Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis.” EMBO J. 22, 5260-5272.
  4. Mizukoshi, T., Tanaka, T., Arai, K., Kohda, D. and *Masai, H. (2003)  “A critical role of the 3’-terminus of nascent DNA chains in recognition of stalled replication forks.” J. Biol. Chem. 278, 42234-42239.
  5. You, Z., Ishimi, Y., Mizuno, T., Sugasawa, K., Hanaoka, F. and *Masai, H. (2003) “Thymine-rich single-stranded DNA sequences specifically activate mouse Mcm4/6/7 helicase on Y-fork and bubble-like substrates.” EMBO J. 22, 6148-6160.
  6. Furukohri, A., Sato, N., Masai, H., Arai, K., Sugino, A. and *Waga, S. (2003) “Identification and characterization of a Xenopus homolog of dbf4, a regulatory subunit of the cdc7 protein kinase required for the initiation of DNA.” J. Biochem. (Tokyo). 134, 447-457.

-2002-

  1. Kim, J. M., Nakao, K., Nakamura, K., Saito, I., Katsuki, M., Arai, K., and *Masai, H. (2002) “Inactivation of Cdc7 kinase in mouse embryonic stem cells results in S phase arrest and p53-dependent cell death.” EMBO J. 21, 2168-2179. (“Exceptional” evaluation in F1000)
  2. Yamada, M., Sato, N., Taniyama, C., Ohtani, K., Arai, K., and *Masai, H. (2002) “A 63 base-pair DNA segment containing a Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase.” J. Biol. Chem. 277, 27668-27681.
  3. Tanaka, T., Mizukoshi, T., Taniyama, C., Kohda, D., Arai, K., and *Masai, H. (2002) “DNA binding of PriA protein requires cooperation of the N-terminal D-loop/arrested-fork binding and C-terminal helicase domains.” J. Biol. Chem. 277, 38062-38071.
  4. *You, Z.,  Ishimi, Y., Masai, H., and Hanaoka, F. (2002) “Roles of Mcm7 and Mcm4 subunits in DNA helicase activity of mouse Mcm4/6/7 complex.” J. Biol. Chem. 277, 42471-42479.

-2001-

  1. Takeda, T., Ogino, K., Tatebayashi, K. Ikeda, H., Arai, K., and *Masai, H. (2001) “Regulation of initiation of DNA replication and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.” Mol. Biol. Cell 12, 1257-1274.
  2. Uchiyama, M., Griffiths, D., Arai, K., and *Masai, H. (2001) “Essential role of Cdc45 in the loading of DNA polymerase a into MCM in fission yeast.”  J. Biol. Chem. 276, 26189-26196.
  3. Uchiyama, M., Arai, K., and Masai, H. (2001) “A novel fission yeast G1 arrest mutant sna41goa1 defective in a Cdc45 homologue interacts genetically with DNA polymerase a.” Mol. Genet. Genom. 265, 1039-1049.
  4. Ogino, K., Takeda, T., Matsui, E., Iiyama, H., Taniyama, C., Arai, K., and *Masai, H. (2001) “Bipartite binding of a kinase activator activates Cdc7-related kinase essential for S phase.” J. Biol. Chem. 276, 31376-31387.
  5. *Ishimi, Y., Komamura-Kohno, Y., Arai, K. and Masai, H. (2001) “Biochemical activities associated with mouse Mcm2 protein.” J. Biol. Chem. 276, 42744-42752.

-2000-

  1. *Masai, H., Matsui, E., You, Z., Ishimi, Y., Tamai, K., and Arai, K. (2000) “Human Cdc7-related kinase complex: In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a critical threonine residue of Cdc7 by Cdks.” J. Biol. Chem. 275, 29042-29052.
  2. *Masai, H. and Arai, K. (2000) “Dbf4 motifs: conserved motifs in activation subunits for Cdc7 kinases essential for S-phase.” Biochem. Biophys. Res. Commun. 18, 228-232.

-1999以前の一部-

  1. Masai, H., Kaziro, Y., and *Arai, K. (1983) “Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.” Proc. Natl. Acad. Sci. USA 80, 6814-6818.
  2. Masai, H., Bond, M.W., and *Arai, K. (1986) “Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.” Proc. Natl. Acad. Sci. USA 83, 1256-1260.
  3. Masai, H. and *Arai, K. (1987) “RepA and DnaA proteins are required for initiation of R1 plasmid replication in vitro and interact with the oriR sequence.” Proc. Natl. Acad. Sci. USA 84, 4781-4785.
  4. *Masai, H., and Arai, K. (1988) “RepA protein- and oriR– dependent initiation of R1 plasmid replication: identification of a rho-dependent transcription terminator required for cis-action of repA protein.” Nucleic Acids Res. 16, 6493-6514.
  5. *Masai, H. and Arai, K. (1989) “Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR.” J. Biol. Chem. 264, 8082-8090.
  6. *Masai, H., Nomura, N. and Arai, K. (1990) “The ABC-primosome: a novel priming system employing dnaA, dnaB, dnaC, and primase on a hairpin containing a dnaA box sequence.” J. Biol. Chem. 265, 15134-15144.
  7. *Masai, H., Asai, T., Kubota, Y., Arai, K. and Kogoma, T. (1994) “Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replications.” EMBO J. 13, 5338-5345.
  8. *Masai, H., Miyake, T. and Arai, K. (1995) “hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7 kinase, is required for chromosomal replication.” EMBO J. 14 3094-3104.
  9.  Sato, N., Arai, K. and *Masai, H. (1997) “Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7.” EMBO J. 16, 4340-4351.
  10. *Masai, H. and Arai, K. (1997) “Frpo, a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication.”  Cell 89, 897-907.
  11. Kumagai, H., Sato, N., Yamada, M., Mahony, D., Seghezzi, W., Lees, E., Arai, K., and *Masai, H. (1999) “A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells.” Mol. Cell. Biol. 19, 5083-5095.
  12.  Takeda, T., Ogino, K., Matsui, E., Cho, M-K., Kumagai, H., Miyake, T., Arai, K., and *Masai, H. (1999) “A Fission yeast gene, him1+/dfp1+, encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S phase initiation as well as in S phase checkpoint control and recovery from DNA damages.” Mol. Cell. Biol. 19, 5535-5547.

 

Reviews and Book chapters (in English only) To view crick here first!

-2022-

  1. Hsiao H-W, Yang C-C, *Masai H (2021) Roles of Claspin in regulation of DNA replication, replication stress responses and oncogenesis in human cells. Genome Instability & Disease 2: 263–280. doi.org/10.1007/s42764-021-00049-8
  2. Masai, H. (2022) TT-pocket/HIRAN: binding to 3’-terminus of DNA for recognition and processing of stalled replication forks. J. Biochem. 172(2):57-60. doi: 10.1093/jb/mvac042.
  3. Masai, H. (2022) Replicon hypothesis revisited Biochemical and Biophysical Research Communications, in press

-2021-

  1. Oki M, *Masai H. (2021) “Regulation of HP1 protein by phosphorylation during transcriptional repression and cell cycle.” J. Biochem. in press

-2020-

  1. *Masai, H. and Tan, Z. (2020) “G-quadruplexes: tools, roles, and goals.” Biochemical and Biophysical Research Communications, 531:1-2.
  2. *Masai, H. and Tanaka, T. (2020) “G-quadruplex DNA and RNA: Their roles in regulation of DNA replication and other biological functions.” Biochemical and Biophysical Research Communications, 531:25-38.
  3. Alavi, S., Ghadiri, H., Dabirmanesh, B., Moriyama, K., Khajeh, K., and *Masai, H. (2020) G-quadruplex binding protein Rif1, a key regulator of replication timing. J. Biochem. doi: 10.1093/jb/mvaa128. Epub ahead of print. PMID: 33169133

-2019-

  1. Tanaka, T. and *Masai, H. (2019) “Bacterial primosome.” In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester.
  2. Masai, H. (2019) “For 60th birthday of BBRC: DNA replication factors outside S phase.” Biochemical and Biophysical Research Communications, 520:685-686.

-2018-

  1. *Masai H, Kanoh Y, Moriyama K, Yamazaki S, Yoshizawa N, Matsumoto S. Telomere-binding factors in the regulation of DNA replication. Genes Genet Syst. 92, 119-125. (Review)
  2. Ghadiri, H. Alavi, S., Dabirmanesh, B., Moriyama, K., *Khajeh, K. and Masai, H. “Study Break: Cell Timer/Cell Clock.” Iran Biomed J. 2018 Sep 16. [Epub ahead of print] PubMed PMID: 30218996.

-2017-

  1. Moriyama K, Lai MS, and *Masai H (2017) Interaction of Rif1 Protein with G-Quadruplex in Control of Chromosome Transactions. Adv Exp Med Biol. 1042, 287-310. doi:10.1007/978-981-10-6955-0_14. PubMed PMID: 29357064.
  2. *Masai H, Yang CC, and Matsumoto S. (2017) Mrc1/Claspin: a new role for regulation of origin firing. Curr Genet. 2017 Oct;63(5):813-818. doi:10.1007/s00294-017-0690-y. Epub 2017 Mar 29. Review. PubMed PMID: 28357499. (Review)
  3. *Masai H. (2017) A novel p53-Cdc7 link induced by genotoxic stress.  Cell Cycle 16, 735-736. doi: 10.1080/15384101.2017.1304746. Epub 2017 Mar 15. PubMed PMID: 28296554; PubMed Central PMCID: PMC5405723. (Commentary)
  4. *Masai, H. (2017) “A novel p53-Cdc7 link induced by genotoix stress.” Cell Cycle, in press (News and Views)
  5. *Masai, H. Encyclopedia of Signaling Molecules, 2nd Edition : Dbf4 (2017) in press
  6. *Masai, H. Encyclopedia of Signaling Molecules, 2nd Edition : Cdc7 (2017) in press

-2016-

-2015-

  1. *Masai, H. (2015) “Building up the machinery for DNA replication.” Cell Cycle 14, 3011-3012. (News and Views)
  2. Yoshizawa, N., Yamazaki, S., and *Masai, H. (2015) “Rif1, a conserved chromatin factor regulating DNA replication, DNA repair and transcription.” In The Initiation of DNA Replication in Eukaryotes, Springer
  3. Hayano, M., Matsumoto, S. and *Masai, H. (2015) “DNA Replication Timing: Temporal and Spatial Regulation of Eukaryotic DNA Replication.” in Fumio Hanaoka and Kaoru Sugasawa (Eds): DNA Replication, Recombination, and Repair (Springer)
  4. Naoko Yoshizawa, Satoshi Yamazaki, and *Hisao Masai (2015) “Rif1, a conserved chromatin factor regulating DNA replication, DNA repair and transcription.” In The Initiation of DNA Replication in Eukaryotes, Springer, in press.
  5. *Masai H. (2015) Building up the machinery for DNA replication. Cell Cycle. [Epub ahead of print] PubMed PMID: 26315494. (Commentary) [PubMed]

-2014-

  1. *Hisao Masai (2014) News and Views “ATM in prevention of genomic instability.” Cell Cycle, 13, 882-883. (Commentary) [PubMed]
  2. Claire Renard-Guillet, Yutaka Kanoh, Katsuhiko Shirahige, and *Hisao Masai (2014) “Recent advances in temporal and spatial regulation of eukaryotic DNA replication: From regulated initiation to genome-scale timing program.” Seminars in Cell & Developmental Biology, 30C, 110-120. (Review) [PubMed]
  3. Yoshizawa-Sugata, N. and *Masai, H. (2014) “Cell cycle synchronization and flow cytometry analysis of mammalian cell.” Methods in Molecular Biology, 1170, 279-293. (Review) [PubMed]

-2013-

  1. Yamazaki, Hayano, M., and *Masai, H. (2013) “Replication timing regulation of eukaryotic replicons: Rif1 as a global regulator of replication timing.” Trends in Genetics. 29, 449-460. (Review) [PubMed]
  2. *Masai, H. (2013) “A personal reflection on the Replicon Theory: from R1 plasmid to replication timing regulation in human cells.” J. Mol. Biol. 425, 4663-4672. (Review) [PubMed] [See also here]
  3. Matsumoto, S. and *Masai, H. (2013) “Regulation of chromosome dynamics by Hsk1 kinase.” Biochemical Society Transactions 41, 1712-1719. (Review) [PubMed]

-2012-

  1.  Toh G-T. and *Masai, H. (2012) “Cdc7L1” UCSD-Nature Molecule Pages, Published online: 31 August 2012 | doi:10.6072/H0.MP.A003137.01 (Review)
  2. *Masai, H. (2012) “Cdc7” The Encyclopedia of Signaling Molecules Springer
Reference and Database Publishing (Review)
  3. *Masai, H. (2012) “Dbf4” The Encyclopedia of Signaling Molecules Springer
 Reference and Database Publishing (Review)

-2011-

  1. *Masai, H. (2011) “RecQL4: a helicase linking formation and maintenance of a replication fork.” J. Biochem. 149, 629-631 (commentary)

-2010-

  1. *Masai, H., Matsumoto, S., You, Z., Yoshizawa-Sugata, N., and Oda, M. (2010) Eukaryotic DNA replication; where, when and how? Annual Rev. Biochem. 79, 89-130. (Review)
  2. *Masai, H., Tanaka, T., and Kohda, D. (2010) “Stalled replication forks: Making ends meet for recognition and stabilization.” Bioessays 32, 687-697. (Review)
  3. Tanaka, T. and *Masai, H. (2010) “Bacterial primosome.” In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0001048.pub2 (Review)
  4. Tanaka, T. and *Masai, H. (2010) “Bacterial replication fork: synthesis of lagging strand.” In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0001049.pub2 (Review)
  5. *Masai, H. (2010) “FANCs regulate firing of DNA replication origins.” Cell Cycle 9, 2494. (Commentary)
  6. Vaziri, C. and *Masai, H. (2010) “Integrating DNA replication with Trans-Lesion Synthesis via Cdc7.” Cell Cycle 9, 4818-4823. (Review)

-2009-

  1. Sawa, M., and *Masai, H. (2009) “Drug Design with Cdc7 kinase, a potential novel cancer therapy target.” Drug Design, Development and Therapy 2, 255-264. (Review)
  2. Toh, G.K., and *Masai, H. (2009) “ASK” UCSD-Nature Molecule Pages, Published online: 25 Feb 2009 | doi:10.1038/mp.a000345.01 (Review)

-2008-

  1. Fujii-Yamamoto, H., Yamada. M., and *Masai, H. (2008) “Regulation of DNA replication factors by E2F in cancer and embryonic stem cells.” in “Control of Cellular Physiology by E2F Transcription Factors” Research Signpost 209-221.(Review)
  2. Ito, S., Taniyama, C., Arai, N. and *Masai, H. (2008) “Cdc7 as a potential new target for cancer therapy.” Drug News and Perspectives 21, 481-488. (Review) Featured on the cover of the issue

-2005-

  1. *Masai, H., Zhiying, Y. & Arai, K. (2005) “Control of DMA replication: regulation and activation of the eukaryotic replicative helicase, MCM.” IUBMB Life 57, 323-335. (Review)

-2004-

  1. Kim, J-M. and *Masai, H. (2004) “Genetic dissection of mammalian Cdc7 kinase: Cell cycle and developmental roles.” Cell Cycle 3, 300-304. (Review)

-2003-

  1. Kim, J-M., Yamada, M. and *Masai, H. (2003) Review: “Defect in Cdc7 kinase leads to checkpoint responses and p53-dependent cell death in mammalian cells.” Mutation Research 532, 29-40.

-2002-

  1. *Masai, H. and Arai, K. (2002) Review with a cover photo: “Cdc7 kinase complex: A key regulator for initiation of DNA replication.” J. Cell. Physiol. 190, 287-296. (Review)

-2001-

  1. *Masai, H. (2001) Bacterial replication fork: synthesis of lagging strand, Encyclopedia of Life Sciences (Macmilan Reference Limited) (Review)
  2. *Masai, H. (2001) Bacterial primosome, Encyclopedia of Life Sciences (Macmilan Reference Limited) (Review)

-2000-

  1. *Johnstone, L., Masai, H. and Sugino, A. (2000) Review: “A Cdc7p-Dbf4p protein kinase activity is conserved from yeast to humans.” Prog. Cell Cycle Res. 4, 61-69.
  2. *Masai, H. and Arai, K. (2000) Review: “Regulation of DNA replication during cell cycle: Roles of Cdc7 kinase and coupling of replication, recombination, and repair in response to replication fork arrest.” IUBMB Life 49, 353-364.

-1999-

  1. *Johnstone, L., Masai, H. and Sugino, A. (1999) Review: “First the Cdk’s, now the Ddk’s.” Trends in Cell Biology 9, 249-252
  2. *Masai, H., Sato, N., Takeda, T. and Arai, K. (1999) Review: “Cdc7/Dbf4-related kinase complex as a molecular switch for initiation of DNA replication.” Frontiers in Bioscience 4, 834-840.

-1996-

  1. *Masai, H. and Arai, K. (1996) Review: “DnaA- and PriA-dependent primosomes: two distinct replication complexes for replication of Escherichia coli chromosome.” Frontiers in Bioscience 1, 49-59.
  2. *Masai, H. and Arai, K. (1996) “Mechanisms of primer RNA synthesis and D-loop/R-loop-dependent DNA replication of Escherichia coli.” Biochimie 78, 1109-1117. (Review)

-1994-

  1. *Masai, H. and Arai, K. (1994) Review: “Replication and transcription on single-stranded DNA templates: proteins that specifically recognize single-stranded DNA sequences with a higher order structure.” Mol. Biol. (Life Sci. Adv.) 13, 19-37.

-1988-

  1. Masai, H. and *Arai, K. (1988) Book chapter: “R1 plasmid replication in vitro: repA- and dnaA-dependent initiation at oriR” in DNA Replication and Mutagenesis Edited by R. E. Moses and W. C. Summers, American Society for Microbiology, Washington, pp 113-121. (Review)