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論文
- タイトル
- タイトル（英）
- DUX4 is a multifunctional factor priming human embryonic genome activation.
- 参照URL
- https://researchmap.jp/hkawaji/published_papers/37017418
- 著者
- 著者（英）
- Sanna Vuoristo,Shruti Bhagat,Christel Hydén-Granskog,Masahito Yoshihara,Lisa Gawriyski,Eeva-Mari Jouhilahti,Vipin Ranga,Mahlet Tamirat,Mikko Huhtala,Ida Kirjanov,Sonja Nykänen,Kaarel Krjutškov,Anastassius Damdimopoulos,Jere Weltner,Kosuke Hashimoto,Gaëlle Recher,Sini Ezer,Priit Paluoja,Pauliina Paloviita,Yujiro Takegami,Ai Kanemaru,Karolina Lundin,Tomi T Airenne,Timo Otonkoski,Juha S Tapanainen,Hideya Kawaji,Yasuhiro Murakawa,Thomas R Bürglin,Markku Varjosalo,Mark S Johnson,Timo Tuuri,Shintaro Katayama,Juha Kere
- 担当区分
- 概要
- 概要（英）
- Double homeobox 4 (DUX4) is expressed at the early pre-implantation stage in human embryos. Here we show that induced human DUX4 expression substantially alters the chromatin accessibility of non-coding DNA and activates thousands of newly identified transcribed enhancer-like regions, preferentially located within ERVL-MaLR repeat elements. CRISPR activation of transcribed enhancers by C-terminal DUX4 motifs results in the increased expression of target embryonic genome activation (EGA) genes ZSCAN4 and KHDC1P1. We show that DUX4 is markedly enriched in human zygotes, followed by intense nuclear DUX4 localization preceding and coinciding with minor EGA. DUX4 knockdown in human zygotes led to changes in the EGA transcriptome but did not terminate the embryos. We also show that the DUX4 protein interacts with the Mediator complex via the C-terminal KIX binding motif. Our findings contribute to the understanding of DUX4 as a regulator of the non-coding genome.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- iScience
- 巻
- 25
- 号
- 4
- 開始ページ
- 104137
- 終了ページ
- 104137
- 出版年月
- 2022年4月15日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.isci.2022.104137
- 共同研究・競争的資金等の研究課題
研究者
川路 英哉
( )