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論文
- タイトル
- SUG1, a component of the 26 S proteasome, is an ATPase stimulated by specific RNAs
- タイトル（英）
- SUG1, a component of the 26 S proteasome, is an ATPase stimulated by specific RNAs
- 参照URL
- https://researchmap.jp/yamano_K/published_papers/38943570
- 著者
- Makino Y,Yamano K,Kanemaki M,Morikawa K,Kishimoto T,Shimbara N,Tanaka K,Tamura T
- 著者（英）
- Makino Y,Yamano K,Kanemaki M,Morikawa K,Kishimoto T,Shimbara N,Tanaka K,Tamura T
- 担当区分
- 概要
- 概要（英）
- SUG1 is an integral component of the 26 S proteasome. Belonging to a novel putative ATPase family, it shares four conserved motifs characteristic of ATP-dependent DNA/RNA helicases. Recombinant rat SUG1 (rSUG1) produced in Escherichia coli was highly purified and characterized in terms of its biochemical properties. The rSUG1 exhibited a Mg2+-dependent ATPase activity. The K(m) for ATP and V(max) of rSUG1 were 35 μM and 7 pmol of ATP/min/μg of protein, respectively. Both ATPase activity to release [32P]monophosphate and [32P]ATP-labeling activity were coordinately affected by cold ATP severely, GTP and UTP moderately, and CTP little. Interestingly, the rSUG1 ATPase activity was stimulated by poly(U) and poly(C), but not by poly(A), poly(G), or by any forms of DNAs tested. A UV cross-linking assay also indicated poly(U)- and poly(C)-stimulated labeling of rSUG1 with [α-32P]ATP. Moreover, the ATPase activity was facilitated by cellular poly(A)+ RNA, but not by poly(A)- RNA. RNA transcribed in vitro from cDNA encoding a b-Zip protein could stimulate the ATPase activity. This is the first report to demonstrate a specific RNA requirement for ATPase with respect to the proteasomal ATPases. Our present work suggests that SUG1 can specifically interact with protein-coding RNA (mRNA) and play some roles in mRNA metabolism.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- Journal of Biological Chemistry
- 誌名（英）
- Journal of Biological Chemistry
- 巻
- 272
- 号
- 37
- 開始ページ
- 23201
- 終了ページ
- 23205
- 出版年月
- 1997年
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0021-9258
- DOI URL
- https://doi.org/10.1074/jbc.272.37.23201
- 共同研究・競争的資金等の研究課題
研究者
山野 晃史
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