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論文
- タイトル
- タイトル（英）
- An arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase
- 参照URL
- https://researchmap.jp/N-Matsuda/published_papers/39986746
- 著者
- 著者（英）
- T Ueda,N Matsuda,T Anai,H Tsukaya,H Uchimiya,A Nakano
- 担当区分
- 概要
- 概要（英）
- The Arabidopsis Ara proteins belong to the Rab/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated a clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the nab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdi1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.
- 出版者・発行元
- 出版者・発行元（英）
- AMER SOC PLANT PHYSIOLOGISTS
- 誌名
- 誌名（英）
- PLANT CELL
- 巻
- 8
- 号
- 11
- 開始ページ
- 2079
- 終了ページ
- 2091
- 出版年月
- 1996年11月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 1040-4651
- DOI URL
- https://doi.org/10.2307/3870414
- 共同研究・競争的資金等の研究課題
研究者
松田 憲之
(マツダ ノリユキ)