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論文
- タイトル
- タイトル（英）
- DNA aptamers against FokI nuclease domain for genome editing applications.
- 参照URL
- https://researchmap.jp/daisukematsumoto/published_papers/27442867
- 著者
- 著者（英）
- Maui Nishio,Daisuke Matsumoto,Yoshio Kato,Koichi Abe,Jinhee Lee,Kaori Tsukakoshi,Ayana Yamagishi,Chikashi Nakamura,Kazunori Ikebukuro
- 担当区分
- 概要
- 概要（英）
- Genome editing with site-specific nucleases (SSNs) can modify only the target gene and may be effective for gene therapy. The main limitation of genome editing for clinical use is off-target effects; excess SSNs in the cells and their longevity can contribute to off-target effects. Therefore, a controlled delivery system for SSNs is necessary. FokI nuclease domain (FokI) is a common DNA cleavage domain in zinc finger nuclease (ZFN) and transcription activator-like effector nuclease. Previously, we reported a zinc finger protein delivery system that combined aptamer-fused, double-strand oligonucleotides and nanoneedles. Here, we report the development of DNA aptamers that bind to the target molecules, with high affinity and specificity to the FokI. DNA aptamers were selected in six rounds of systematic evolution of ligands by exponential enrichment. Aptamers F6#8 and #71, which showed high binding affinity to FokI (Kd=82nM, 74nM each), showed resistance to nuclease activity itself and did not inhibit nuclease activity. We immobilized the ZFN-fused GFP to nanoneedles through these aptamers and inserted the nanoneedles into HEK293 cells. We observed the release of ZFN-fused GFP from the nanoneedles in the presence of cells. Therefore, these aptamers are useful for genome editing applications such as controlled delivery of SSNs.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Biosensors & bioelectronics
- 巻
- 93
- 号
- 開始ページ
- 26
- 終了ページ
- 31
- 出版年月
- 2017年7月15日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.bios.2016.11.042
- 共同研究・競争的資金等の研究課題
研究者
松本 大亮
(マツモト ダイスケ)