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論文
- タイトル
- タイトル（英）
- Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs
- 参照URL
- https://researchmap.jp/satide/published_papers/33983812
- 著者
- 著者（英）
- Satoru Ide#*,Asuka Sasaki#,Yusuke Kawamoto,Toshikazu Bando,Hiroshi Sugiyama,Kazuhiro Maeshima
- 担当区分
- 筆頭著者, 責任著者
- 概要
- 概要（英）
- <title>Abstract</title><sec> <title>Background</title> Knowing chromatin components at a DNA regulatory element at any given time is essential for understanding how the element works during cellular proliferation, differentiation and development. A region-specific chromatin purification is an invaluable approach to dissecting the comprehensive chromatin composition at a particular region. Several methods (e.g., PICh, enChIP, CAPTURE and CLASP) have been developed for isolating and analyzing chromatin components. However, all of them have some shortcomings in identifying non-coding RNA associated with DNA regulatory elements. </sec><sec> <title>Results</title> We have developed a new approach for affinity purification of specific chromatin segments employing an <italic>N</italic>-methyl pyrrole (P)-<italic>N</italic>-methylimidazole (I) (PI) polyamide probe, which binds to a specific sequence in double-stranded DNA via Watson–Crick base pairing as a minor groove binder. This new technique is called proteomics and RNA-omics of isolated chromatin segments (PI-PRICh). Using PI-PRICh to isolate mouse and human telomeric components, we found enrichments of shelterin proteins, the well-known telomerase RNA component (TERC) and telomeric repeat-containing RNA (TERRA). When PI-PRICh was performed for alternative lengthening of telomere (ALT) cells with highly recombinogenic telomeres, in addition to the conventional telomeric chromatin, we obtained chromatin regions containing telomeric repeat insertions scattered in the genome and their associated RNAs. </sec><sec> <title>Conclusion</title> PI-PRICh reproducibly identified both the protein and RNA components of telomeric chromatin when targeting telomere repeats. PI polyamide is a promising alternative to simultaneously isolate associated proteins and RNAs of sequence-specific chromatin regions under native conditions, allowing better understanding of chromatin organization and functions within the cell. </sec>
- 出版者・発行元
- 出版者・発行元（英）
- Springer Science and Business Media LLC
- 誌名
- 誌名（英）
- Epigenetics & Chromatin
- 巻
- 14
- 号
- 1
- 開始ページ
- 46
- 終了ページ
- 46
- 出版年月
- 2021年12月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1186/s13072-021-00421-8
- 共同研究・競争的資金等の研究課題
研究者
井手 聖
(イデ サトル)