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論文
- タイトル
- タイトル（英）
- Bcl2l12, a novel protein interacting with Arf6, triggers Schwann cell differentiation programme.
- 参照URL
- https://researchmap.jp/351K/published_papers/48937345
- 著者
- 著者（英）
- Wakana Miyata,Naoko Sakaibara,Kentaro Yoshinaga,Asahi Honjo,Mikito Takahashi,Tatsuya Ooki,Hideji Yako,Kazunori Sango,Yuki Miyamoto,Junji Yamauchi
- 担当区分
- 概要
- 概要（英）
- Schwann cells are glial cells in the peripheral nervous system (PNS); they wrap neuronal axons with their differentiated plasma membranes called myelin sheaths. Although the physiological functions, such as generating saltatory conduction, have been well studied in the PNS, the molecular mechanisms by which Schwann cells undergo their differentiation programme without apparent morphological changes before dynamic myelin sheath formation remain unclear. Here, for the first time, we report that Arf6, a small GTP/GDP-binding protein controlling morphological differentiation, and the guanine-nucleotide exchange factors cytohesin proteins are involved in the regulation of Schwann cell differentiation marker expression in primary Schwann cells. Specific inhibition of Arf6 and cytohesins by NAV-2729 and SecinH3, respectively, decreased expression of marker proteins 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and glial fibrillary acidic protein (GFAP). Similar results using promoter assays were observed using the IMS32 Schwann cell line. Furthermore, using an affinity-precipitation technique, we identified Bcl2-like 12 (Bcl2l12) as a novel GTP-bound Arf6-interacting protein. Knockdown of Bcl2l12 using a specific artificial miRNA decreased expression of marker proteins. The knockdown also led to decreased filamentous actin extents. These results suggest that Arf6 and Bcl2l12 can trigger Schwann cell differentiation, providing evidence for a molecular relay that underlies how Schwann cells differentiate.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Journal of biochemistry
- 巻
- 177
- 号
- 1
- 開始ページ
- 5
- 終了ページ
- 14
- 出版年月
- 2025年1月6日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1093/jb/mvae078
- 共同研究・競争的資金等の研究課題