※ をクリックすると外部の論文のサイトが開きます。
論文
- タイトル
- タイトル（英）
- Single Dose of Attenuated Vaccinia Viruses Expressing H5 Hemagglutinin Affords Rapid and Long-Term Protection Against Lethal Infection with Highly Pathogenic Avian Influenza A H5N1 Virus in Mice and Monkeys.
- 参照URL
- https://researchmap.jp/read0088287/published_papers/49006171
- 著者
- 著者（英）
- Fumihiko Yasui,Keisuke Munekata,Tomoko Fujiyuki,Takeshi Kuraishi,Kenzaburo Yamaji,Tomoko Honda,Sumiko Gomi,Misako Yoneda,Takahiro Sanada,Koji Ishii,Yoshihiro Sakoda,Hiroshi Kida,Shosaku Hattori,Chieko Kai,Michinori Kohara
- 担当区分
- 筆頭著者, 責任著者
- 概要
- 概要（英）
- BACKGROUND/OBJECTIVES: In preparation for a potential pandemic caused by the H5N1 highly pathogenic avian influenza (HPAI) virus, pre-pandemic vaccines against several viral clades have been developed and stocked worldwide. Although these vaccines are well tolerated, their immunogenicity and cross-reactivity with viruses of different clades can be improved. METHODS: To address this aspect, we generated recombinant influenza vaccines against H5-subtype viruses using two different strains of highly attenuated vaccinia virus (VACV) vectors. RESULTS: rLC16m8-mcl2.2 hemagglutinin (HA) and rLC16m8-mcl2.3.4 HA consisted of a recombinant LC16m8 vector encoding the HA protein from clade 2.2 or clade 2.3.4 viruses (respectively); rDIs-mcl2.2 HA consisted of a recombinant DIs vector encoding the HA protein from clade 2.2. A single dose of rLC16m8-mcl2.2 HA showed rapid (1 week after vaccination) and long-term protection (20 months post-vaccination) in mice against the HPAI H5N1 virus. Moreover, cynomolgus macaques immunized with rLC16m8-mcl2.2 HA exhibited long-term protection when challenged with a heterologous clade of the HPAI H5N1 virus. Although the DIs strain is unable to grow in most mammalian cells, rDIs-mcl2.2 HA also showed rapid and long-lasting effects against HPAI H5N1 virus infection. Notably, the protective efficacy of rDIs-mcl2.2 HA was comparable to that of rLC16m8-mcl2.2 HA. Furthermore, these vaccines protected animals previously immunized with VACVs from a lethal challenge with the HPAI H5N1 virus. CONCLUSIONS: These results suggest that both rLC16m8-mcl2.2 HA and rDIs-mcl2.2 HA are effective in preventing HPAI H5N1 virus infection, and rDIs-mcl2.2 HA is a promising vaccine candidate against H5 HA-subtype viruses.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Vaccines
- 巻
- 13
- 号
- 1
- 開始ページ
- 終了ページ
- 出版年月
- 2025年1月15日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.3390/vaccines13010074
- 共同研究・競争的資金等の研究課題