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論文
- タイトル
- タイトル（英）
- Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis.
- 参照URL
- https://researchmap.jp/Yoshiaki_Kikkawa/published_papers/48530167
- 著者
- 著者（英）
- Masatoshi Fukuda,Hiroki Okanishi,Daisuke Ino,Kazuya Ono,Takeru Ota,Eri Wakai,Takashi Sato,Yumi Ohta,Yoshiaki Kikkawa,Hidenori Inohara,Yoshikatsu Kanai,Hiroshi Hibino
- 担当区分
- 概要
- 概要（英）
- The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α2-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α2-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- iScience
- 巻
- 27
- 号
- 11
- 開始ページ
- 111214
- 終了ページ
- 111214
- 出版年月
- 2024年11月15日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.isci.2024.111214
- 共同研究・競争的資金等の研究課題
研究者
吉川 欣亮
(キッカワ ヨシアキ)