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論文
- タイトル
- タイトル（英）
- Saffold virus exploits integrin αvβ8 and sulfated glycosaminoglycans as cooperative attachment receptors for infection
- 参照URL
- https://researchmap.jp/kkoba/published_papers/51864702
- 著者
- 著者（英）
- Takako Okuwa*,Toshiki Himeda*,Kyousuke Kobayashi*,Namiko Nomura,Kouichi Utani,Satoshi Koike,Akira Nakamura,Masaya Higuchi *cofirst author
- 担当区分
- 筆頭著者
- 概要
- 概要（英）
- Saffold virus (SAFV), a member of the species Cardiovirus saffoldi within the Picornaviridae family, causes acute respiratory and gastrointestinal illnesses as well as hand, foot, and mouth disease. It is also suspected to be associated with neuronal disorders, such as encephalitis and meningitis, in severe cases. Despite its clinical significance, the virus-host interactions underlying SAFV pathogenicity remain largely unknown. Using a genome-wide CRISPR-Cas9 knockout screen, we identify the following receptors for SAFV infection: sulfated glycosaminoglycans (GAGs) and integrin αVβ8. Single knockouts of SLC35B2, an essential gene for sulfated GAG synthesis, or the integrin genes ITGAV or ITGB8 partially reduce SAFV-3 and SAFV-2 susceptibility in HeLa cells, and a double knockout confers complete resistance. Furthermore, we demonstrate that SAFV-3 virions bind directly to sulfated GAGs and integrin αVβ8. Based on these findings, we propose a model of SAFV infection in which sulfated GAGs and integrin αVβ8 act through dual and cooperative pathways to facilitate viral entry.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Nature Communications
- 巻
- 17
- 号
- 1
- 開始ページ
- 534
- 終了ページ
- 534
- 出版年月
- 2025年12月15日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1038/s41467-025-67236-z
- 共同研究・競争的資金等の研究課題
研究者
小林 郷介
( )