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論文
- タイトル
- タイトル（英）
- Liquid-like transcription condensates locally constrain chromatin in living human cells
- 参照URL
- https://researchmap.jp/satide/published_papers/52466583
- 著者
- 著者（英）
- Adilgazy Semeigazin,Katsuhiko Minami,Masa A. Shimazoe,Saadi Khochbin,Daniel Panne,Satoru Ide,Kazuhiro Maeshima
- 担当区分
- 概要
- 概要（英）
- The organization and dynamics of chromatin play important roles in transcriptional regulation. The transcription machinery is known to constrain chromatin dynamics. Recently, transcription condensates formed via liquid-liquid phase separation (LLPS) or other mechanisms have emerged as key regulators of gene expression. What is the physical nature of such condensates in the cell? Do they interact with and constrain chromatin? To address these questions, we focused on BRD4-NUT, a fusion oncoprotein found in NUT carcinoma. Using single-molecule dual-color imaging, we found that individual BRD4-NUT molecules diffuse within condensates like a viscous liquid in live human cells. Single-nucleosome imaging specific to euchromatin shows that these liquid-like condensates restrict the movement of chromatin through BRD4 bromodomain-dependent crosslinking of neighboring acetylated nucleosomes. Our findings uncover a previously unrecognized mechanism by which LLPS-based condensates modulate chromatin dynamics, suggesting that condensates contribute to genome regulation through physical, and not only biochemical, control.
- 出版者・発行元
- 出版者・発行元（英）
- Cold Spring Harbor Laboratory
- 誌名
- 誌名（英）
- 巻
- 号
- 開始ページ
- 終了ページ
- 出版年月
- 2025年7月9日
- 査読の有無
- 招待の有無
- 掲載種別
- ISSN
- DOI URL
- https://doi.org/10.1101/2025.07.05.663270
- 共同研究・競争的資金等の研究課題
研究者
井手 聖
(イデ サトル)