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論文
- タイトル
- タイトル（英）
- Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1.
- 参照URL
- https://researchmap.jp/toyonatsume/published_papers/35823886
- 著者
- 著者（英）
- Anika Wiegard,Vladislav Kuzin,Donald P Cameron,Jan Grosser,Michele Ceribelli,Rashid Mehmood,Roberto Ballarino,Francesco Valant,Radosław Grochowski,Ivana Karabogdan,Nicola Crosetto,Arne Lindqvist,Anna Helene Bizard,Fedor Kouzine,Toyoaki Natsume,Laura Baranello
- 担当区分
- 概要
- 概要（英）
- As cells enter mitosis, chromatin compacts to facilitate chromosome segregation yet remains transcribed. Transcription supercoils DNA to levels that can impede further progression of RNA polymerase II (RNAPII) unless it is removed by DNA topoisomerase 1 (TOP1). Using ChIP-seq on mitotic cells, we found that TOP1 is required for RNAPII translocation along genes. The stimulation of TOP1 activity by RNAPII during elongation allowed RNAPII clearance from genes in prometaphase and enabled chromosomal segregation. Disruption of the TOP1-RNAPII interaction impaired RNAPII spiking at promoters and triggered defects in the post-mitotic transcription program. This program includes factors necessary for cell growth, and cells with impaired TOP1-RNAPII interaction are more sensitive to inhibitors of mTOR signaling. We conclude that TOP1 is necessary for assisting transcription during mitosis with consequences for growth and gene expression long after mitosis is completed. In this sense, TOP1 ensures that cellular memory is preserved in subsequent generations.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Molecular cell
- 巻
- 号
- 開始ページ
- 終了ページ
- 出版年月
- 2021年11月5日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.molcel.2021.10.015
- 共同研究・競争的資金等の研究課題
研究者
夏目 豊彰
(ナツメ トヨアキ)