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論文
- タイトル
- タイトル（英）
- Not core 2 β1,6-N-acetylglucosaminyltransferase-2 or -3 but -1 regulates sialyl-Lewis x expression in human precursor B cells
- 参照URL
- https://researchmap.jp/s-takaku/published_papers/35991055
- 著者
- 著者（英）
- Jiro Kikuchi,Hirotaka Shinohara,Chizu Nonomura,Hidenobu Ando,Shizuka Takaku,Hisao Nojiri,Mitsuru Nakamura
- 担当区分
- 概要
- 概要（英）
- Sialyl-Lewis x (sLeX), one of the major selectin ligands, is expressed on T and B cells in a differentiation or activation stage-specific manner. We have demonstrated before that sLeX expression and core 2 β1,6-N-acetylglucosaminyl-transferase (C2GnT) were simultaneously regulated during precursor B (pre-B) cell differentiation. Three C2GnT family genes, designated C2GnT-1, -2, and -3, were previously identified, but their roles have not been fully examined. In this study, we have investigated the roles of C2GnTs in the regulation of sLeX expression level during pre-B cell differentiation comparing with α1,3fucosyltransferase-VII (FucT-VII) and α2,3sialyltransferase-IV (ST3Gal-IV). Overexpression of not FucT-VII and ST3Gal-IV but C2GnT-1 blocked the down-regulation of sLeX expression by differentiation induction. Neither C2GnT-2 nor -3 but C2GnT-1 transcript was mainly expressed in B lineage cell lines and bone marrow-derived B lineage cells. Significant down-regulation of C2GnT-1 of the three C2GnTs was observed in KM3 cells during differentiation. The expression of C2GnT-1 correlated well to sLeX expression and differentiation stage. Furthermore, introduction of short interfering RNA against C2GnT-1 markedly reduced C2GnT-1 expression and resulted in down-regulation of sLeX expression. These results suggest that not the other glycosyl-transferases but C2GnT-1 regulates sLeX expression level during differentiation of pre-B cells, providing the cells with substrate of sLeX structure biosynthesis. © Oxford University Press 2005; all rights reserved.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Glycobiology
- 巻
- 15
- 号
- 3
- 開始ページ
- 271
- 終了ページ
- 280
- 出版年月
- 2005年3月
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0959-6658
- DOI URL
- https://doi.org/10.1093/glycob/cwi005
- 共同研究・競争的資金等の研究課題
研究者
高久 静香
(タカク シズカ)