※ をクリックすると外部の論文のサイトが開きます。
論文
- タイトル
- タイトル(英)
- In vivo role of phosphorylation of cryptochrome 2 in the mouse circadian clock.
- 参照URL
- https://researchmap.jp/n_kurabayashi/published_papers/28778875
- 著者
- 著者(英)
- Arisa Hirano,Nobuhiro Kurabayashi,Tomoki Nakagawa,Go Shioi,Takeshi Todo,Tsuyoshi Hirota,Yoshitaka Fukada
- 担当区分
- 概要
- 概要(英)
- The circadian clock is finely regulated by posttranslational modifications of clock components. Mouse CRY2, a critical player in the mammalian clock, is phosphorylated at Ser557 for proteasome-mediated degradation, but its in vivo role in circadian organization was not revealed. Here, we generated CRY2(S557A) mutant mice, in which Ser557 phosphorylation is specifically abolished. The mutation lengthened free-running periods of the behavioral rhythms and PER2::LUC bioluminescence rhythms of cultured liver. In livers from mutant mice, the nuclear CRY2 level was elevated, with enhanced PER2 nuclear occupancy and suppression of E-box-regulated genes. Thus, Ser557 phosphorylation-dependent regulation of CRY2 is essential for proper clock oscillation in vivo.
- 出版者・発行元
- 出版者・発行元(英)
- 誌名
- 誌名(英)
- Molecular and cellular biology
- 巻
- 34
- 号
- 24
- 開始ページ
- 4464
- 終了ページ
- 73
- 出版年月
- 2014年12月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文(学術雑誌)
- ISSN
- DOI URL
- https://doi.org/10.1128/MCB.00711-14
- 共同研究・競争的資金等の研究課題
研究者
倉林 伸博
(クラバヤシ ノブヒロ)