※ をクリックすると外部の論文のサイトが開きます。
論文
- タイトル
- タイトル（英）
- Infant Antibody Repertoires during the First Two Years of Influenza Vaccination.
- 参照URL
- https://researchmap.jp/Tanno/published_papers/40561833
- 著者
- 著者（英）
- Masayuki Kuraoka,Nicholas C Curtis,Akiko Watanabe,Hidetaka Tanno,Seungmin Shin,Kevin Ye,Elizabeth Macdonald,Olivia Lavidor,Susan Kong,Tarra Von Holle,Ian Windsor,Gregory C Ippolito,George Georgiou,Emmanuel B Walter,Garnett Kelsoe,Stephen C Harrison,M Anthony Moody,Goran Bajic,Jiwon Lee
- 担当区分
- 概要
- 概要（英）
- The first encounter with influenza virus biases later immune responses. This "immune imprinting," formerly from infection within a few years of birth, is in the United States now largely from immunization with a quadrivalent, split vaccine (IIV4 [quadrivalent inactivated influenza vaccine]). In a pilot study of IIV4 imprinting, we used single-cell cultures, next-generation sequencing, and plasma antibody proteomics to characterize the primary antibody responses to influenza in two infants during their first 2 years of seasonal influenza vaccination. One infant, who received only a single vaccination in year 1, contracted an influenza B virus (IBV) infection between the 2 years, allowing us to compare imprinting by infection and vaccination. That infant had a shift in hemagglutinin (HA)-reactive B cell specificity from largely influenza A virus (IAV) specific in year 1 to IBV specific in year 2, both before and after the year 2 vaccination. HA-reactive B cells from the other infant maintained a more evenly distributed specificity. In year 2, class-switched HA-specific B cell IGHV somatic hypermutation (SHM) levels reached the average levels seen in adults. The HA-reactive plasma antibody repertoires of both infants comprised a relatively small number of antibody clonotypes, with one or two very abundant clonotypes. Thus, after the year 2 boost, both infants had overall B cell profiles that resembled those of adult controls. IMPORTANCE Influenza virus is a moving target for the immune system. Variants emerge that escape protection from antibodies elicited by a previously circulating variant ("antigenic drift"). The immune system usually responds to a drifted influenza virus by mutating existing antibodies rather than by producing entirely new ones. Thus, immune memory of the earliest influenza virus exposure has a major influence on later responses to infection or vaccination ("immune imprinting"). In the many studies of influenza immunity in adult subjects, imprinting has been from an early infection, since only in the past 2 decades have infants received influenza immunizations. The work reported in this paper is a pilot study of imprinting by the flu vaccine in two infants, who received the vaccine before experiencing an influenza virus infection. The results suggest that a quadrivalent (four-subtype) vaccine may provide an immune imprint less dominated by one subtype than does a monovalent infection.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- mBio
- 巻
- 号
- 開始ページ
- e0254622
- 終了ページ
- 出版年月
- 2022年10月31日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1128/mbio.02546-22
- 共同研究・競争的資金等の研究課題
研究者
丹野 秀崇
(タンノ ヒデタカ)