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論文
- タイトル
- タイトル（英）
- Gender-Difference in Hair Length as Revealed by Crispr-Based Production of Long-Haired Mice with Dysfunctional FGF5 Mutations.
- 参照URL
- https://researchmap.jp/takahashi-gou/published_papers/40831667
- 著者
- 著者（英）
- Ryo Takahashi,Gou Takahashi,Yuichi Kameyama,Masahiro Sato,Masato Ohtsuka,Kenta Wada
- 担当区分
- 概要
- 概要（英）
- Fibroblast growth factor 5 (FGF5) is an important molecule required for the transition from anagen to catagen phase of the mammalian hair cycle. We previously reported that Syrian hamsters harboring a 1-bp deletion in the Fgf5 gene exhibit excessive hair growth in males. Herein, we generated Fgf5 mutant mice using genome editing via oviductal nucleic acid delivery (GONAD)/improved GONAD (i-GONAD), an in vivo genome editing system used to target early embryos present in the oviductal lumen, to study gender differences in hair length in mutant mice. The two lines (Fgf5go-malc), one with a 2-bp deletion (c.552_553del) and the other with a 1-bp insertion (c.552_553insA) in exon 3 of Fgf5, were successfully established. Each mutation was predicted to disrupt a part of the FGF domain through frameshift mutation (p.Glu184ValfsX128 or p.Glu184ArgfsX128). Fgf5go-malc1 mice had heterogeneously distributed longer hairs than wild-type mice (C57BL/6J). Notably, this change was more evident in males than in females (p < 0.0001). Immunohistochemical analysis revealed the presence of FGF5 protein in the dermal papilla and outer root sheath of the hair follicles from C57BL/6J and Fgf5go-malc1 mice. Histological analysis revealed that the prolonged anagen phase might be the cause of accelerated hair growth in Fgf5go-malc1 mice.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- International journal of molecular sciences
- 巻
- 23
- 号
- 19
- 開始ページ
- 終了ページ
- 出版年月
- 2022年10月6日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.3390/ijms231911855
- 共同研究・競争的資金等の研究課題
研究者
高橋 剛
(タカハシ ゴウ)