{"id":2637,"date":"2019-05-07T12:45:08","date_gmt":"2019-05-07T03:45:08","guid":{"rendered":"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2637"},"modified":"2019-05-07T13:50:33","modified_gmt":"2019-05-07T04:50:33","slug":"%e7%a0%94%e7%a9%b6%e3%82%a8%e3%83%83%e3%82%bb%e3%82%a4","status":"publish","type":"page","link":"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2637","title":{"rendered":"\u7814\u7a76\u30a8\u30c3\u30bb\u30a4"},"content":{"rendered":"<div id=\"toc-np-container\"><p id=\"toc-np-title\">Contents<\/p><ul class=\"no-bullets\"><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2637#Discovery-of-G-quadruplex-as-a-major-determinant-for-replication-timing-regulation-Rif1-recognizes-and-binds-to-a-special-DNA-structure-to-generate-a-specific-chromatin-architecture\">Discovery of G-quadruplex as a major determinant for replication timing regulation: Rif1 recognizes and binds to a special DNA structure to generate a specific chromatin architecture<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2637#A-personal-reflection-on-the-Replicon-Theory5032DNA\">A personal reflection on the Replicon Theory\u30ec\u30d7\u30ea\u30b3\u30f3\u30e2\u30c7\u30eb\u3068\u3068\u3082\u306b50\u5e74\uff08\u79c1\u306e32\u5e74\u9593\u306eDNA\u8907\u88fd\u7814\u7a76\u3092\u632f\u308a\u8fd4\u3063\u3066\uff09<\/a><\/li><\/ul><\/div><h2><span id=\"Discovery-of-G-quadruplex-as-a-major-determinant-for-replication-timing-regulation-Rif1-recognizes-and-binds-to-a-special-DNA-structure-to-generate-a-specific-chromatin-architecture\">Discovery of G-quadruplex as a major determinant for replication timing regulation: Rif1 recognizes and binds to a special DNA structure to generate a specific chromatin architecture<\/span><\/h2>\n<h5>\u00a0DNA\u306e\u9ad8\u6b21\u69cb\u9020\u3092\u4ecb\u3057\u305f\u3001\u30b2\u30ce\u30e0\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u65b0\u3057\u3044\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u767a\u898b<\/h5>\n<p><strong>\u301c\u304c\u3093\u3084\u75be\u60a3\u306e\u65b0\u3057\u3044\u539f\u56e0\u89e3\u660e\u306b\u671f\u5f85\u301c<\/strong><\/p>\n<p>\u5f53\u7814\u7a76\u5ba4\u306e\u52a0\u7d0d\u8c4a\u4e3b\u4efb\u7814\u7a76\u54e1\u3089\u306f\u3001\u30b2\u30ce\u30e0\u8907\u88fd\u5236\u5fa1\u6a5f\u69cb\u306e\u89e3\u6790\u304b\u3089\u3001\u30b2\u30ce\u30e0\u4e0a\u306b\u5b58\u5728\u3059\u308b\u7279\u6b8aDNA\u9ad8\u6b21\u69cb\u9020\u3092\u4ecb\u3057\u3066\u3001\u30b2\u30ce\u30e0\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u65b0\u3057\u3044\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u6210\u679c\u306f\u3001\u30b2\u30ce\u30e0\u6a5f\u80fd\u5236\u5fa1\u306e\u65b0\u3057\u3044\u539f\u7406\u306e\u89e3\u660e\u306b\u7e4b\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3068\u3068\u3082\u306b\u3001\u304c\u3093\u3084\u7a2e\u3005\u306e\u75be\u60a3\u306e\u539f\u56e0\u89e3\u660e\u306b\u65b0\u3057\u3044\u9053\u3092\u958b\u304f\u3082\u306e\u3067\u3059\u3002<\/p>\n<p>\u672c\u7814\u7a76\u306f\u3001\u6771\u4eac\u8fb2\u5de5\u5927\u306e\u9577\u6fa4\u548c\u592b\u6559\u6388\u3001\u6176\u61c9\u5927\u5b66\u306e\u6cb3\u91ce \u66a2\u660e\u535a\u58eb\u3001\u6771\u4eac\u5927\u5b66\u306e\u767d\u9aed\u514b\u5f66\u6559\u6388\u3068\u306e\u5171\u540c\u7814\u7a76\u306b\u3088\u308a\u3001\u65e5\u672c\u5b66\u8853\u632f\u8208\u4f1a\u79d1\u7814\u8cbb\uff08\u57fa\u76e4\u7814\u7a76\u3001\u65b0\u5b66\u8853\u9818\u57df\u7814\u7a76\uff09\u306e\u4e00\u74b0\u3068\u3057\u3066\u884c\u308f\u308c\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u6210\u679c\u306f\u30012015\u5e74\u306b\u82f1\u56fd\u79d1\u5b66\u8a8c\u300eNature Structural &amp; Molecular Biology\uff08\u30cd\u30fc\u30c1\u30e3\u30fc\u30b9\u30c8\u30e9\u30af\u30c1\u30e5\u30e9\u30eb\u30a2\u30f3\u30c9\u30e2\u30ec\u30ad\u30e5\u30e9\u30fc\u30d0\u30a4\u30aa\u30ed\u30b8\u30fc\uff09\u300f\u306b\u30aa\u30f3\u30e9\u30a4\u30f3\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"text-decoration: underline;\">\u00a0\uff11\u3000\u7814\u7a76\u306e\u80cc\u666f<\/span><\/p>\n<p>\u30b2\u30ce\u30e0\u3092\u69cb\u6210\u3059\u308bDNA\u306f\u901a\u5e38\u3001B\u578b\u306e\uff12\u91cd\u3089\u305b\u3093\u69cb\u9020<sup>*1<\/sup>\u3092\u3068\u308a\u307e\u3059\u3002\u3053\u306e\u69cb\u9020\u306f\u5b89\u5b9a\u3067\u307b\u3068\u3093\u3069\u306e\u30b2\u30ce\u30e0\u9818\u57df\u306f\u3053\u306e\u5f62\u3092\u3068\u3063\u3066\u3044\u308b\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u4ee5\u524d\u304b\u3089DNA\u306f\uff13\u91cd\u9396\u3001\uff14\u91cd\u9396\u3001\u5de6\u5dfb\u304dDNA\u3001\u5341\u5b57\u67b6\u69cb\u9020\u306a\u3069B\u578b\u306e\uff12\u91cd\u3089\u305b\u3093\u69cb\u9020\u4ee5\u5916\u306e\u69cb\u9020\u3092\u3068\u308a\u5f97\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u69cb\u9020\u306f\u8a66\u9a13\u7ba1\u5185\u3067\u6df1\u304f\u89e3\u6790\u3055\u308c\u307e\u3057\u305f\u304c\u3001\u751f\u4f53\u5185\u3067\u306e\u610f\u7fa9\u306f\u4e0d\u660e\u3067\u3057\u305f\u3002\u7279\u306b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020<sup>*2<\/sup>(\u4ee5\u964dG4\u69cb\u9020\u3068\u8a18\u8f09\u3057\u307e\u3059)\u306f80\u5e74\u4ee3\u672b\u306e\u767a\u898b\u4ee5\u6765\u591a\u304f\u306e\u7814\u7a76\u304c\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u30c6\u30ed\u30e1\u30a2<sup>*3<\/sup>\u914d\u5217\u5185\u3067\u306eG4\u69cb\u9020\u914d\u5217\u306e\u5b58\u5728\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u610f\u7fa9\u306f\u660e\u3089\u304b\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002G4\u69cb\u9020\u3092\u5f62\u6210\u3057\u3046\u308b\u914d\u5217\u306f\u5927\u8178\u83cc\u30b2\u30ce\u30e0\u306b6\u5343\u30ab\u6240\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u306b35\u4e07\u30ab\u6240\u4ee5\u4e0a\u5b58\u5728\u3059\u308b\u3068\u63a8\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001\u6700\u8fd1\u7279\u7570\u7684\u306a\u6297\u4f53\u3084\u3001\u7d50\u5408\u5316\u5408\u7269\u3092\u7528\u3044\u3066\u3001\u5b9f\u969b\u306bG4\u69cb\u9020\u304c\u6838\u306e\u4e2d\u306b\u5b58\u5728\u3059\u308b\u3053\u3068\u3082\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001G4\u69cb\u9020\u306f\u8907\u88fd\u3092\u963b\u5bb3\u3057\u30b2\u30ce\u30e0\u5909\u52d5\u3092\u8a98\u5c0e\u3059\u308b\u4e8b\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001G4\u69cb\u9020\u306e\u666e\u904d\u7684\u306a\u6a5f\u80fd\u306b\u3064\u3044\u3066\u306f\u4e0d\u660e\u3067\u3057\u305f\u3002\u6b63\u4e95\u7814\u7a76\u54e1\u3089\u306f\u3001\u907a\u4f1d\u5b50\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u306a\u3044\u9818\u57df\u306b\u5b58\u5728\u3059\u308bG4\u69cb\u9020\u304c\u3001\u7279\u7570\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u8a8d\u8b58\u3055\u308c\u3001\u67d3\u8272\u4f53\u3092\u5bc4\u305b\u96c6\u3081\u3066\u3001\u8907\u88fd\u306e\u30bf\u30a4\u30df\u30f3\u30b0<sup>*4<\/sup>\u306a\u3069\u3092\u6c7a\u5b9a\u3059\u308b\u67d3\u8272\u4f53\u306e\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3<sup>*5<\/sup>\u3092\u5f62\u6210\u3059\u308b\u53ef\u80fd\u6027\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u7533\u8acb\u8005\u306e\u767a\u898b\u306fG4\u69cb\u9020\u306e\u666e\u904d\u7684\u304b\u3064\u4fdd\u5b58\u3055\u308c\u305f\u751f\u7269\u5b66\u7684\u6a5f\u80fd\u306e\u89e3\u660e\u306b\u9032\u6b69\u3092\u3082\u305f\u3089\u3059\u3082\u306e\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"text-decoration: underline;\">\uff12\u3000\u7814\u7a76\u306e\u6982\u8981<\/span><\/p>\n<p>\u79c1\u305f\u3061\u306f\u3001\u8907\u88fd\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u6c7a\u5b9a\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u6790\u3059\u308b\u904e\u7a0b\u3067\u3001Rif1\u3068\u3044\u3046\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u306a\u304f\u306a\u308b\u3068\u3001\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u306e\u5236\u5fa1\u304c\u5931\u308f\u308c\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u3066\u5831\u544a\u3057\u3066\u304d\u307e\u3057\u305f\uff08Genes Dev 2012; EMBO J 2012\uff09\u3002Rif1\u306f\u9175\u6bcd\u304b\u3089\u30d2\u30c8\u307e\u3067\u4fdd\u5b58\u3055\u308c\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3001\u6838\u306b\u5b58\u5728\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7<sup>*7<\/sup>\u3092\u5f62\u6210\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u306e\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3068\u60f3\u50cf\u3055\u308c\u307e\u3057\u305f\uff08\u56f31\uff09\u3002\u305d\u3053\u3067\u3001\u672c\u7814\u7a76\u3067\u306f\u3001\u307e\u305a\u5206\u88c2\u9175\u6bcd\u30b2\u30ce\u30e0\u4e0a\u306b\u304a\u3051\u308bRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u90e8\u4f4d\u3092ChIP-Seq\u6cd5<sup>*8<\/sup>\u306b\u3088\u308a\u6c7a\u5b9a\u3057\u307e\u3057\u305f(\u56f32)\u3002\u305d\u306e\u7d50\u679c12.5Mb\u306e\u30b2\u30ce\u30e0\u4e0a\u306b35\u500b\u306e\u5f37\u3044\u7d50\u5408\u90e8\u4f4d\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306f\u3044\u305a\u308c\u3082\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3057\u3001\u60c5\u5831\u89e3\u6790\u304b\u3089\u3001S\u671f\u5f8c\u671f\u306b\u958b\u59cb\u3059\u308b\u8907\u88fd\u8d77\u70b9\u306e\u8fd1\u508d\u306b\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u7d50\u5408\u90e8\u4f4d\u306e\u914d\u5217\u89e3\u6790\u304b\u3089\u3001G\u306e\u9023\u7d9a\u914d\u5217\u3092\u542b\u3080\u4fdd\u5b58\u3055\u308c\u305f\u914d\u5217\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u4fdd\u5b58\u914d\u5217\u306e\u307b\u304b\u306b\u3082Rif1\u7d50\u5408\u90e8\u4f4d\u306fG\u306e\u9023\u7d9a\u914d\u5217\u3092\u8907\u6570\u500b\u542b\u3080\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u30b2\u30ce\u30e0\u4e0a\u306eRif1\u7d50\u5408\u914d\u5217\u4e0a\u306e\u4fdd\u5b58\u914d\u5217\u306b\u5909\u7570\u3092\u5c0e\u5165\u3057\u305f\u7d50\u679c\u3001Rif1\u306e\u30af\u30ed\u30de\u30c1\u30f3\u7d50\u5408\u304c\u5c40\u6240\u7684\u306b\u6d88\u5931\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u305d\u306e\u8fd1\u508d\u306e\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u304c\u8131\u5236\u5fa1\u3055\u308c\u3001\u8907\u88fd\u8d77\u70b9\u304cS\u671f\u521d\u671f\u306b\u6d3b\u6027\u5316\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u540c\u5b9a\u3057\u305f\u4fdd\u5b58\u914d\u5217\u304cRif1\u306e\u30af\u30ed\u30de\u30c1\u30f3\u7d50\u5408\u3068Rif1\u306e\u6a5f\u80fd\u767a\u73fe\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<p>\u6b21\u306b\u3001Rif1\u304c\u3069\u306e\u3088\u3046\u306a\u4fdd\u5b58\u914d\u5217\u306b\u4f9d\u5b58\u3057\u3066Rif1\u7d50\u5408\u9818\u57df\u306b\u7d50\u5408\u3059\u308b\u304b\u3092\u751f\u5316\u5b66\u7684\u306b\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u7cbe\u88fd\u3057\u305fRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\uff12\u672c\u9396DNA\u306b\u7d50\u5408\u3059\u308b\u304c\u3001Rif1\u4fdd\u5b58\u914d\u5217\u3078\u306e\u4f9d\u5b58\u6027\u306f\u89b3\u5bdf\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002G\u306e\u9023\u7d9a\u914d\u5217\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304b\u3089\u3001G4\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u304b\u3069\u3046\u304b\u3092\u8abf\u3079\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001Rif1\u7d50\u5408\u914d\u5217\u3092\u71b1\u5909\u6027\u3057\u3066\u3001\u518d\u71b1\u51e6\u7406\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u4fdd\u5b58\u914d\u5217\u306b\u4f9d\u5b58\u3057\u3066G4\u69d8\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u7cbe\u88fd\u3057\u305fRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001G4\u69cb\u9020\u3092\u5f62\u6210\u3057\u305f\u6642\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3092\u8a3c\u660e\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u308c\u3089\u306e\u767a\u898b\u306f\u3001\u7d30\u80de\u5185\u3067G4\u69cb\u9020\u304c\u5b9f\u969b\u306b\u5f62\u6210\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u3092\u7279\u7570\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d50\u5408\u3057\u3001\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u907a\u4f1d\u5b66\u7684\u306b\u8a3c\u660e\u3059\u308b\u3082\u306e\u3067\u3059(\u56f33)\u3002<\/p>\n<p>G4\u69cb\u9020\u306e\u67d3\u8272\u4f53\u5236\u5fa1\u306b\u304a\u3051\u308b\u666e\u904d\u7684\u306a\u5f79\u5272\u306f\u3053\u308c\u307e\u3067\u5168\u304f\u4e0d\u660e\u3067\u3057\u305f\u304c\u3001\u4eca\u56de\u306e\u767a\u898b\u306f\u3001G4\u69cb\u9020\u304c\u67d3\u8272\u4f53\u4e0a\u306e\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3057\u3001\u305d\u308c\u3092\u7279\u7570\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u8a8d\u8b58\u3001\u7d50\u5408\u3057\u3001\u67d3\u8272\u4f53\u306e\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u53ef\u80fd\u6027\u3092\u793a\u3059\u3082\u306e\u3067\u3059(\u56f34)\u3002\u3053\u308c\u307e\u3067\u672a\u77e5\u3067\u3042\u3063\u305fG4\u69cb\u9020\u306e\u666e\u904d\u7684\u306a\u751f\u7269\u5b66\u7684\u610f\u7fa9\u306e\u4e00\u7aef\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3082\u306e\u3068\u3057\u3066\u7279\u7b46\u3055\u308c\u308b\u6210\u679c\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"text-decoration: underline;\">\uff13\u3000\u4eca\u5f8c\u306e\u5c55\u671b<\/span><\/p>\n<p>\u4e0a\u8ff0\u306e\u3088\u3046\u306bG4\u69cb\u9020\u306f\u30d2\u30c8\u30b2\u30ce\u30e0\u4e0a\u306b40\u4e07\u500b\u8fd1\u304f\u5b58\u5728\u3059\u308b\u3068\u60f3\u50cf\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u3089\u306f\u3001\u8907\u88fd\u3001\u8ee2\u5199\u3001\u7d44\u63db\u3048\u3001\u306a\u3069\u591a\u69d8\u306a\u67d3\u8272\u4f53\u5236\u5fa1\u306b\u95a2\u4e0e\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u56de\u306e\u767a\u898b\u306fRif1\u3068\u3044\u3046\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u3001G4\u69cb\u9020\u306b\u7d50\u5408\u3057\u3001\u67d3\u8272\u4f53\u69cb\u9020\u306e\u5236\u5fa1\u3092\u4ecb\u3057\u3066\u8907\u88fd\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002Rif1\u306f\u8ee2\u5199\u306b\u3082\u5927\u304d\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3059\u3053\u3068\u3092\u79c1\u305f\u3061\u306f\u3059\u3067\u306b\u898b\u51fa\u3057\u3066\u304a\u308a\u3001Rif1\u7d50\u5408\u90e8\u4f4d\u3067\u3042\u308bG4\u69cb\u9020\u306f\u3001\u305d\u308c\u304c\u5909\u7570\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306b\u3082\u5927\u304d\u306a\u5f71\u97ff\u304c\u53ca\u307c\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u304c\u3093\u3084\u7a2e\u3005\u306e\u75be\u60a3\u3067\u306f\u30b2\u30ce\u30e0\u5909\u7570\u306b\u3088\u308a\u3001\u8ee2\u5199\u3084\u8907\u88fd\u5236\u5fa1\u304c\u7570\u5e38\u306b\u306a\u3063\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u305d\u306e\u3088\u3046\u306a\u30b2\u30ce\u30e0\u5909\u7570\u304c\u3001G4\u69cb\u9020\u306a\u3069DNA\u306e\u9ad8\u6b21\u69cb\u9020\u306b\u5909\u5316\u3092\u53ca\u307c\u3059\u7d50\u679c\u3001\u8ee2\u5199\u3084\u8907\u88fd\u306e\u7570\u5e38\u3092\u3082\u305f\u3089\u3057\u75be\u60a3\u306e\u539f\u56e0\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u5f8c\u306f\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u4e0a\u306b\u3001\u3069\u306e\u3088\u3046\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u3067\u3001\u307e\u305f\u3069\u306e\u3088\u3046\u306a\u5834\u6240\u306bG4\u69cb\u9020\u304c\u5f62\u6210\u3055\u308c\u308b\u304b\u3001\u3055\u3089\u306b\u305d\u308c\u3089\u304c\u30b2\u30ce\u30e0\u306e\u8ee2\u5199\u3001\u8907\u88fd\u3001\u7d44\u63db\u3048\u3001\u4fee\u5fa9\u3092\u5236\u5fa1\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30b2\u30ce\u30e0\u6a5f\u80fd\u306e\u5236\u5fa1\u306e\u65b0\u3057\u3044\u539f\u7406\u306e\u89e3\u660e\u306b\u3064\u306a\u304c\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\u3055\u3089\u306b\u3001G4\u69cb\u9020\u306a\u3069DNA\u9ad8\u6b21\u69cb\u9020\u5f62\u6210\u90e8\u4f4d\u306e\u5909\u7570\u3068\u75be\u60a3\u306e\u95a2\u9023\u306b\u3064\u3044\u3066\u89e3\u6790\u3057\u3001\u305d\u308c\u3089\u306e\u5909\u7570\u3092\u6a19\u7684\u3068\u3057\u305f\u65b0\u3057\u3044\u8a3a\u65ad\u6cd5\u3001\u6cbb\u7642\u6cd5\u306e\u958b\u767a\u3092\u76ee\u6307\u3057\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>[\u7528\u8a9e\u8aac\u660e]<\/p>\n<p>*1 B\u578b\u306e\uff12\u91cd\u3089\u305b\u3093\u69cb\u9020<\/p>\n<p>\u30ef\u30c8\u30bd\u30f3\u30fc\u30af\u30ea\u30c3\u30af\u304c\u767a\u898b\u3057\u305f\uff12\u672c\u9396DNA\u306e\u57fa\u672c\u7684\u69cb\u9020\u3002\u30a2\u30c7\u30cb\u30f3-\u30c1\u30df\u30f3\u304a\u3088\u3073\u30b0\u30a2\u30cb\u30f3-\u30b7\u30c8\u30b7\u304c\u6c34\u7d20\u7d50\u5408\u3067\u30da\u30a2\uff08\u5869\u57fa\u5bfe\uff09\u3092\u5f62\u6210\u3057\u3001\u53f3\u5dfb\u304d\uff12\u91cd\u3089\u305b\u3093\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u3002\u7d30\u80de\u5185\u306eDNA\u306e\u57fa\u672c\u69cb\u9020\u3067\u3042\u308b\u3002<\/p>\n<p>*2\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020<\/p>\n<p>\uff14\u500b\u306e\u30b0\u30a2\u30cb\u30f3\u304c\u3001\u5e73\u9762\u4e0a\u3067\u6b63\u65b9\u5f62\u306e\u5404\u9802\u70b9\u306e\u4f4d\u7f6e\u306b\u914d\u4f4d\u3057\u3066\u3044\u308b\u3002\u4e2d\u592e\u306b\u306f\u5fc5\u9808\u306eK+\u30a4\u30aa\u30f3\u304c\u5b58\u5728\u3059\u308b\u3002\u3053\u306e\u5e73\u9762\u304c\uff13\u679a\u91cd\u306a\u3063\u305f\u72b6\u614b\u306b\u306a\u3063\u3066\uff14\u91cd\u9396\u3092\u5f62\u6210\u3059\u308b\u3002\u57fa\u672c\u7684\u306b\u306fGGG\u304c\u3001\u3042\u308b\u7a0b\u5ea6\u306e\u9593\u9694\u3067\uff14\u56de\u73fe\u308c\u308b\u3068\u5f62\u6210\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002GGG\u3092\u9023\u7d50\u3059\u308b\u30eb\u30fc\u30d7\u306e\u9577\u3055\u306f\u4e0d\u5b9a\u3067\u3042\u308b\u3002<\/p>\n<p>*3\u30c6\u30ed\u30e1\u30a2<\/p>\n<p>\u67d3\u8272\u4f53\u306e\u672b\u7aef\u306e\u3053\u3068\u3002\u591a\u304f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30c6\u30ed\u30e1\u30a2\u306b\u96c6\u7a4d\u3057\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u67d3\u8272\u4f53\u306e\u672b\u7aef\u3092\u4fdd\u8b77\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>*4\u8907\u88fd\u306e\u30bf\u30a4\u30df\u30f3\u30b0<\/p>\n<p>6-8\u6642\u9593\u7d99\u7d9a\u3059\u308bS(DNA\u5408\u6210)\u671f\u306b\u304a\u3044\u3066\u3001\u30b2\u30ce\u30e0\u306e\u3069\u306e\u90e8\u4f4d\u304c\u3044\u3064\u8907\u88fd\u3059\u308b\u304b\u3068\u3044\u3046\u30eb\u30fc\u30eb\u3002<\/p>\n<p>*5\u67d3\u8272\u4f53\u306e\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3<\/p>\n<p>\u8ee2\u5199\u3001\u3042\u308b\u3044\u306f\u8907\u88fd\u306a\u3069\u306b\u304a\u3044\u3066\u3001\u3072\u3068\u307e\u3068\u3081\u3067\u5236\u5fa1\u3055\u308c\u308b\u67d3\u8272\u4f53\u9818\u57df\u306e\u5358\u4f4d<\/p>\n<p>*6\u8907\u88fd\u8d77\u70b9<\/p>\n<p>DNA\u8907\u88fd\u304c\u958b\u59cb\u3059\u308b\u67d3\u8272\u4f53\u9818\u57df<\/p>\n<p>*7\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7<\/p>\n<p>\u67d3\u8272\u4f53\u3092\u4e00\u7b87\u6240\u3067\u675f\u306d\u3066\u5f62\u6210\u3055\u308c\u308b\u30eb\u30fc\u30d7\u72b6\u306e\u69cb\u9020\u3002<\/p>\n<p>*8 ChIP-Seq\u6cd5<\/p>\n<p>Chromatin immunoprecipitation-Sequencing\u3002\u67d3\u8272\u4f53\u514d\u75ab\u6c88\u964d\u2014\u6b21\u4e16\u4ee3\u5869\u57fa\u914d\u5217\u6c7a\u5b9a\u6cd5\u3002\u67d3\u8272\u4f53\u306b\u7d50\u5408\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u514d\u75ab\u6c88\u964d\u306b\u3088\u308a\u305d\u306e\u7d50\u5408\u914d\u5217DNA\u3068\u3068\u3082\u306b\u6fc3\u7e2e\u3057\u3001\u305d\u308c\u3089\u306eDNA\u3092\u76f4\u63a5\u5869\u57fa\u914d\u5217\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u9818\u57df\u3092\u6c7a\u5b9a\u3059\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u767a\u8868\u96d1\u8a8c<\/p>\n<p>Nature Structural &amp; Molecular Biology<\/p>\n<p>Article<\/p>\n<p>\uff1c\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb\uff1e<\/p>\n<p>Rif1 binds to G-quadruplexes and suppresses replication over a long distances<\/p>\n<p>Rif1\u306f\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u306b\u7d50\u5408\u3057\u3001\u7d50\u5408\u90e8\u4f4d\u306e\u8fd1\u508d\u306e\u9577\u3044\u9818\u57df\u306e\u8907\u88fd\u3092\u6291\u5236\u3059\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2577 size-large alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/54a05bcfaf668e0ef49bf0e0b7d82822-620x417.jpg\" alt=\"HP \u30a8\u30c3\u30bb\u30a4 \u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u56f31\" width=\"620\" height=\"417\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/54a05bcfaf668e0ef49bf0e0b7d82822-620x417.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/54a05bcfaf668e0ef49bf0e0b7d82822-300x202.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/54a05bcfaf668e0ef49bf0e0b7d82822.jpg 708w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p>\u56f31\u00a0Rif1\u306f\u6838\u819c\u4e0a\u306e\u6838\u9aa8\u683c\u7d50\u5408\u9818\u57df\u306b\u7d50\u5408\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u69cb\u9020\u306e\u5f62\u6210\u3092\u4ecb\u3057\u3066\u4e2d\u671f\u30fb\u5f8c\u671f\u8907\u88fd\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/3e41aa77610f1514d0e651b2456a6c02.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2576 size-full alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/8d9f84122f5b275ba3c0f4d0083e0243.jpg\" alt=\"HP\u30a8\u30c3\u30bb\u30a4\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u56f32\" width=\"494\" height=\"374\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/8d9f84122f5b275ba3c0f4d0083e0243.jpg 494w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/8d9f84122f5b275ba3c0f4d0083e0243-300x227.jpg 300w\" sizes=\"(max-width: 494px) 100vw, 494px\" \/><\/a><\/p>\n<p>\u56f32\u3000\u7814\u7a76\u306e\u6d41\u308c<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-2575 size-large\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/3e41aa77610f1514d0e651b2456a6c02-620x461.jpg\" alt=\"HP\u30a2\u30c3\u30c3\u30bb\u30a4\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u56f33\" width=\"620\" height=\"461\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/3e41aa77610f1514d0e651b2456a6c02-620x461.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/3e41aa77610f1514d0e651b2456a6c02-300x223.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/3e41aa77610f1514d0e651b2456a6c02.jpg 708w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>\u56f3\uff13\u3000Rif1\u306f\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3059\u308bG4\u69cb\u9020\u306b\u7d50\u5408\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u3092\u675f\u306d\u308b\u3068\u3068\u3082\u306b\u6838\u819c\u8fd1\u508d\u306b\u8907\u88fd\u6291\u5236\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2574 size-full alignleft\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/289dbf2f2331b20f0e2e5c30a26556a0.jpg\" alt=\"HP\u30a8\u30c3\u30bb\u30a4\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u56f34\" width=\"584\" height=\"373\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/289dbf2f2331b20f0e2e5c30a26556a0.jpg 584w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2019\/04\/289dbf2f2331b20f0e2e5c30a26556a0-300x192.jpg 300w\" sizes=\"(max-width: 584px) 100vw, 584px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u56f3\uff14\u3000\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u306e\u751f\u7269\u5b66\u7684\u610f\u7fa9\uff1a\u4eca\u56de\u3001\u67d3\u8272\u4f53\u306e\u9ad8\u6b21\u69cb\u9020\u306e\u5f62\u6210\u5236\u5fa1\u3000\u3068\u3044\u3046\u666e\u904d\u7684\u306a\u6a5f\u80fd\u306e\u4e00\u7aef\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002<\/p>\n<h2><span id=\"A-personal-reflection-on-the-Replicon-Theory5032DNA\">A personal reflection on the Replicon Theory\u30ec\u30d7\u30ea\u30b3\u30f3\u30e2\u30c7\u30eb\u3068\u3068\u3082\u306b50\u5e74\uff08\u79c1\u306e32\u5e74\u9593\u306eDNA\u8907\u88fd\u7814\u7a76\u3092\u632f\u308a\u8fd4\u3063\u3066\uff09<\/span><\/h2>\n<p>Celebrating<b> the 50<sup>th<\/sup>\u00a0anniversary of the Replicon Theory<\/b><br \/>\nMarch 25-28, 2013 at the Institut Pasteur,\u00a0Paris,\u00a0France<\/p>\n<p>2013\u5e74\u306fJacob, Brenner, Cousin\u304c\u30ec\u30d7\u30ea\u30b3\u30f3\u4eee\u8aac\u3092\u63d0\u5531\u3057\u306650\u5e74\u306e\u7bc0\u76ee\u306e\u5e74\u3067\u3042\u3063\u305f\u3002\u3053\u308c\u3092\u8a18\u5ff5\u3057\u3066\u30013\/25-28\u307e\u3067\u4e0a\u8a18\u306e\u30b7\u30f3\u30dd\u30b8\u30a6\u30e0\u304c\u3001\u30d1\u30b9\u30c4\u30fc\u30eb\u7814\u7a76\u6240\u3067\u958b\u50ac\u3055\u308c\u305f(\u5199\u771f1,2)\u3002Cuzin\u535a\u58eb\u306f\u307e\u3060\u304a\u5143\u6c17\u3067\u3001\u53c2\u52a0\u3055\u308c\uff08\u5199\u771f3\uff09\u3054\u8b1b\u6f14\u3082\u3055\u308c\u307e\u3057\u305f\u3002DNA\u306e\u534a\u4fdd\u5b58\u7684\u8907\u88fd\u3092density label\u306e\u65b9\u6cd5\u3067\u521d\u3081\u3066\u8a3c\u660e\u3057\u305fMatthew Meselson\u535a\u58eb\u3082\u5927\u5909\u304a\u5143\u6c17\u3067\u4eca\u56de\u306e\u4f1a\u8b70\u306b\u53c2\u52a0\u3055\u308c\u3066\u304a\u308a\u3001\u4eca\u3082\u73fe\u5f79\u3067\u6d3b\u8e8d\u3055\u308c\u3066\u304a\u308a\u307e\u3059\uff08\u5199\u771f4\uff09\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090162.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-723\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090162-300x225.jpg\" alt=\"P1090162\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090162-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090162-1024x768.jpg 1024w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090162.jpg 2048w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090166.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-724\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090166-300x225.jpg\" alt=\"P1090166\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090166-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090166-1024x768.jpg 1024w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090166.jpg 2048w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>\u5199\u771f\uff11\u3000\u30d1\u30b9\u30c4\u30fc\u30eb\u7814\u7a76\u6240\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u3000\u5199\u771f\uff12<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090189.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-726\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090189-300x225.jpg\" alt=\"P1090189\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090189-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090189-1024x768.jpg 1024w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090189.jpg 2048w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>\u5199\u771f\uff13\u3000Cuzin\u535a\u58eb<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090201.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-725\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090201-300x225.jpg\" alt=\"P1090201\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090201-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090201-1024x768.jpg 1024w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/P1090201.jpg 2048w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><span style=\"font-size: 14px;\">\u5199\u771f\uff14\u3000Meselson\u535a\u58eb(\u5de6\u304b\u30892\u756a\u76ee)<\/span><\/p>\n<p>\u3053\u306e\u30b7\u30f3\u30dd\u30b8\u30a6\u30e0\u3092\u8a18\u5ff5\u3057\u3066J.Mol.Biol.\u8a8c\u306b\u304a\u3044\u3066\u7279\u96c6\u53f7\u304c\u7d44\u307e\u308c\u3001\u6b63\u4e95\u30ea\u30fc\u30c0\u30fc\u3082\u5bc4\u7a3f\u3044\u305f\u3057\u307e\u3057\u305f\u3002\u8ad6\u6587\u306f<a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/YJMBI64036.pdf\">\u3053\u3061\u3089<\/a>\u304b\u3089\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"ContentsDiscovery of G-quadruplex as a major determinant for repl<a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2637\">&nbsp;&nbsp;[&#8230;\u7d9a\u304d\u3092\u8aad\u3080]<\/a>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2637"}],"collection":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2637"}],"version-history":[{"count":23,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2637\/revisions"}],"predecessor-version":[{"id":2663,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2637\/revisions\/2663"}],"wp:attachment":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2637"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}