{"id":2804,"date":"2014-03-17T00:00:23","date_gmt":"2014-03-16T15:00:23","guid":{"rendered":"https:\/\/www.igakuken.or.jp\/genome?page_id=2804"},"modified":"2020-08-18T15:09:42","modified_gmt":"2020-08-18T06:09:42","slug":"reserch-2","status":"publish","type":"page","link":"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804","title":{"rendered":"Our Research at a glance"},"content":{"rendered":"<div id=\"toc-np-container\"><p id=\"toc-np-title\">Contents<\/p><ul class=\"no-bullets\"><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#Research-Summary-of-the-Masai-Laboratory\">Research Summary of the Masai Laboratory<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#Current-Research-Activities\">Current Research Activities<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#Future-plans\">Future plans<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#1-Rif1-preferentially-binds-to-parallel-type-and-oligomerized-G-quadruplex-structures\">\u6700\u65b0\u7814\u7a76\u6210\u679c(1) Rif1 preferentially binds to parallel-type and oligomerized G-quadruplex structures<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#2-Regulation-of-chromatin-structure-by-Rif1-through-its-binding-to-G4-at-nuclear-periphery\">\u6700\u65b0\u7814\u7a76\u6210\u679c(2) Regulation of chromatin structure by Rif1 through its binding to G4 at nuclear periphery<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#3-Structures-of-DNA-containing-G4-forming-sequences-on-duplex-DNA\">\u6700\u65b0\u7814\u7a76\u6210\u679c(3) Structures of DNA containing G4-forming sequences on duplex DNA<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#4-Biochemical-characterization-of-mammalian-Rif1-protein\">\u6700\u65b0\u7814\u7a76\u6210\u679c(4) Biochemical characterization of mammalian Rif1 protein<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#5-DNA-strand-annealing-activities-of-Mcm-complexes\">\u6700\u65b0\u7814\u7a76\u6210\u679c(5) DNA strand annealing activities of Mcm complexes<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#6-Negative-regulation-of-origin-firing-by-Mrc1\">\u6700\u65b0\u7814\u7a76\u6210\u679c(6) Negative regulation of origin firing by Mrc1<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#7-Regulation-of-initiation-of-DNA-replication-by-Claspin\">\u6700\u65b0\u7814\u7a76\u6210\u679c(7) Regulation of initiation of DNA replication by Claspin<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#8-Rif1-binds-regulates-replication-timing-through-binding-to-G-quadruplex-structures-on-the-genome\">\u6700\u65b0\u7814\u7a76\u6210\u679c(8) Rif1 binds regulates replication timing through binding to G-quadruplex structures on the genome.<\/a><\/li><li class=\"header-level-1\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804#Graphic-representations-of-recent-results\">Graphic representations of recent results<\/a><\/li><\/ul><\/div><h2><span id=\"Research-Summary-of-the-Masai-Laboratory\">Research Summary of the Masai Laboratory<\/span><\/h2>\n<p>Precise duplication of genetic materials is central to the stable maintenance of genomes through generations. Defects in the genome copying processes would generate genomic instability which could ultimately result in various diseases including cancer. The goal of our studies is to understand the molecular basis of how the huge genomes are precisely replicated and the exact copies of the genetic materials are inherited to the next generation. Three billion base pairs of the human genome (2 meter long) are replicated with almost no errors during the\u00a06-8\u00a0hrs time span of the cell cycle. This requires an extreme level of coordination of temporal and spatial arrangements of chromatin organization and signaling events for initiation of DNA replication (Masai et al. Ann. Rev. Biochem.\u00a0[2010]).\u00a0Our major projects include<\/p>\n<p>1) Maintenance of genome integrity and its failure as a cause of diseases: Molecular dissection of cellular responses to replication stress, a major trigger for oncogenesis, and elucidation of mechanisms by which stalled forks are processed and the genome is protected from various insults, and of how the failure of this process leads to diseases and senescence (genetics and cell biology),<\/p>\n<p>2) Chromosome dynamics that determines cell fate and regulates cell proliferation: Elucidation of mechanisms regulating temporal and spatial regulation of genome duplication as well as coordination of replication, repair, recombination and transcription.\u00a0(genomics\u00a0and molecular genetics; Yamazaki et al. Trends in Genetics [2013]),<\/p>\n<p>3) Unraveling the universal mechanisms of origin firing and its regulation, and evolution of replication systems (molecular biology and enzymology), and<\/p>\n<p>4) DNA replication and development: Understanding the roles of replication factors or replication timing regulation during development\/ differentiation processes of various tissues and organs (mice engineering).<\/p>\n<p>To achieve these goals, we are using E. coli, fission yeast, various mammalian cell lines, embryonic stem cells and model animals. We would like ultimately to apply the basic knowledge on the mechanisms of stable genome maintenance to the diagnosis and therapy of the relevant diseases including cancer. We are recruiting highly motivated and interested individuals who are communicative and can share excitement with us in the laboratory. We have had students from many foreign countries including Korea, Malaysia, Taiwan, China, Canada, Italy, France and Germany and have been excited to have many different cultures in our laboratory. Please feel free to contact us at any time through e-mail or by telephone. (<a href=\"https:\/\/www.igakuken.or.jp\/genome\/\">https:\/\/www.igakuken.or.jp\/genome\/<\/a>)<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"Current-Research-Activities\">Current Research Activities<\/span><\/h2>\n<p>Our goal is to understand the molecular mechanisms underlying the faithful inheritance of genetic materials and stable maintenance of the genome. Toward this goal, we are studying various aspects of chromosome dynamics, with particular focus on regulation during S phase, using &lt;i&gt;E. coli&lt;\/i&gt;, fission yeast, and mammalian cells. We try to elucidate how chromosomes replicate and how the inheritance of the replicated chromosomes is regulated to enable stable maintenance of the genome through generations. Answers to these questions will shed light on how defects in these processes may contribute to the development of diseases, including cancers, and to senescence. It will also help to identify novel target proteins for cancer therapies.&lt;br&gt;Through these studies, we have come to realize the roles of non-B DNA, more specifically, G-quadruple structures. Recent numerous reports indicate the essential roles of various G4 structures in transcription, recombination, genome rearrangement, epigenome regulation and others.<\/p>\n<p>Through analyzing the roles of G4 structures in replication initiation and chromatin regulation, we would like to disclose more general biological functions of G4 structures. &lt;br&gt;DNA replication needs to continue until the entire genome is replicated, once it is initiated. The block to ongoing DNA replication is a threat to the genomic integrity, and needs to be removed swiftly. Claspin\/Mrc1 is a key protein that transmits the replication stress signal to the downstream effectors. We have discovered a novel function of Mrc1\/Claspin in regulation of initiation through interaction with Cdc7 kinase. Furthermore, we have shown that Claspin is required also for different forms of stress. It is needed for cells to resume growth after serum starvation and for their responses to other stresses including osmotic shock and high temperature. We propose that Claspin may act as a general stress response mediator by linking stress-specific PIKK (PI3 kinase related kinase) and downstream effectors. We are interested in the in vivo roles of cell cycle\/ checkpoint factors, and are making mutant mice in which these genes are conditionally knocked out in specific tissues\/ organs by using Cre-loxP system. We are also developing novel anti-cancer strategies utilizeing cell cycle regulators as a target.<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_1English.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-large wp-image-2546 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_1English-620x438.jpg\" alt=\"hisao_1English\" width=\"620\" height=\"438\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_1English-620x438.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_1English-300x212.jpg 300w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><br \/>\nFig. 1 Regulation of chromatin architecture for establishing replication timing domains. Rif1 facilitates chromatin loop formation through its binding to G4 structures present in the intergenic segments and its multimerization activitiy. This will generate chromatin architecture that may be related to the replication timing domain structures.<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/G4-biology.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-large wp-image-2552 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/G4-biology-620x379.jpg\" alt=\"G4 biology\" width=\"620\" height=\"379\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/G4-biology-620x379.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/G4-biology-300x183.jpg 300w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p>Fig. 2 Potential biological roles of G4. Increasing numbers of reports indicate the crucial roles of G4 in various chromosome transactions.<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_3English.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-large wp-image-2544 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_3English-620x378.jpg\" alt=\"hisao_3English\" width=\"620\" height=\"378\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_3English-620x378.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_3English-300x183.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/hisao_3English.jpg 1142w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p>Fig. 3 Roles of Claspin and Cdc7 in regulation of replication initiation and replication stress responses. Cdc7 is recruited to the acidic patch segment of Claspin and facilitates Mcm phosphorylation. Cdc7 also facilitates DNA stress-induced Chk1 activation by promoting Chk1 binding to CKBD.<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"Future-plans\">Future plans<\/span><\/h2>\n<p>In each cell cycle, three billion base pairs of the human genome are replicated within 6\u20138 hrs with amazing accuracy, while initiation takes place at tens of thousands of so called \u201creplication origins\u201d. Selection of replication origins for initiation is regulated by a number of factors, while a large fraction of origin selection may be determined stochastically. Yet, the initiation does take place at sequences that may be associated with some features. Our studies as well those from other groups suggest that G-quadruplex may be a candidate structure that may be associated with replication initiation. Our current goal is to reconstitute \u201chuman-like\u201d replication system in E.coli with purified proteins on G4 sequences. &lt;br&gt; We have already indicated the importance of G4 in establishment of replication timing domain structures by showing that Rif1, a factor crucial for replication timing regulation, binds specifically to G4 DNA. We would like to understand molecular basis on how Rif1 facilitates chromatin loop formation by binding to G4. G4 is associated with other chromosome transactions as well, and a number of proteins bind to G4. We would like to clarify the modes and nature of interaction between G4 and its binding partners, elucidate the mode of actions of these proteins and ultimately obtain global views on potential biological functions of G4. &lt;br&gt;Replication factors are involved not only in the process of DNA replication but also in other aspects of chromosome functions. In order to clarify the organismal roles of replication factors, we target Cdc7, Claspin and other replication factors and generate genetically modified mice. Tissue\/ organ-specific knock-out will reveal their novel functions, and will lead to understanding the pathology of human genetic disorders caused by mutations in these factors. &lt;br&gt;\u201cOncogenic stress\u201d are known to induce replication stress, which inhibits the progression of replication forks. Thus, replication stress triggers the tumorigenesis process. The maintenance of replication fork integrity is central to the stable inheritance of genetic information through generations and to its preservation during the lifespan of an individual. Using mouse model systems as stated above, we will unravel the cellular and developmental roles of replication factors. Ultimately, we aim to apply our expertise on the molecular mechanisms of DNA replication and its physiology to the development of strategies for preventing and treating diseases caused by the loss of genetic integrity.<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"1-Rif1-preferentially-binds-to-parallel-type-and-oligomerized-G-quadruplex-structures\">\u6700\u65b0\u7814\u7a76\u6210\u679c(1) Rif1 preferentially binds to parallel-type and oligomerized G-quadruplex structures<\/span><\/h2>\n<p><strong>Article in Scientific Reports in press<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and\u00a0oligomerization activities.<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Hisao Masai, Rino Fukatsu, Naoko Kakusho, Yutaka Kanoh, Kenji\u00a0Moriyama, Yue Ma, Keisuke Iida, and Kazuo Nagasawa<\/strong><\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/4-20-2019-SR-submitted.pdf\">\u8ad6\u6587\u306epdf<\/a><\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\u672c\u8ad6\u6587\u3067\u306f\u3001\u9032\u5316\u7684\u306b\u4fdd\u5b58\u3055\u308c\u305f\u6838\u56e0\u5b50Rif1\u3068\u3001\u305d\u306e\u6a19\u7684\u3067\u3042\u308b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396(G4)\u69cb\u9020\u306b\u3064\u3044\u3066\u8a73\u7d30\u306a\u89e3\u6790\u3092\u884c\u3063\u305f\u3002Rif1\u306f\u3001parallel \u3042\u308b\u3044\u306f hybrid\/mix\u30bf\u30a4\u30d7\u306eG4\u69cb\u9020\u306b\u3001\u9ad8\u3044\u89aa\u548c\u6027\u3067\u7d50\u5408\u3059\u308b\u3002\u3068\u304f\u306b\u3001\u30aa\u30ea\u30b4\u30de\u30fc\u5316\u3057\u305fG4\u306b\u9078\u629e\u7684\u306b\u7d50\u5408\u3059\u308b\u3002Rif1\u306f\u30018-16\u91cf\u4f53\u3092\u5f62\u6210\u3057\u3001\u8907\u6570\u306eG4\u306b\u540c\u6642\u306b\u7d50\u5408\u3059\u308b\u3002Rif1\u306f\u3001\u8907\u6570\u306e\u30b2\u30ce\u30e0\u4e0a\u306eG4\u69cb\u9020\u3068\u540c\u6642\u306b\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30af\u30ed\u30de\u30c1\u30f3\u3092\u675f\u306d\u3001\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u3000\u3068\u3044\u3046\u30e2\u30c7\u30eb\u3092\u63d0\u5531\u3057\u305f\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001Taz1\u3068DNA\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3064\u3044\u3066\u3082\u3001\u5831\u544a\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8ea7aff1004a2c4aff27a115a2af1338.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-full wp-image-2622 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8ea7aff1004a2c4aff27a115a2af1338.jpg\" alt=\"SR\u8ad6\u6587\u306e\u30e2\u30c7\u30eb\" width=\"578\" height=\"418\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8ea7aff1004a2c4aff27a115a2af1338.jpg 578w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8ea7aff1004a2c4aff27a115a2af1338-300x217.jpg 300w\" sizes=\"(max-width: 578px) 100vw, 578px\" \/><\/a><\/p>\n<p>\u56f3\u3000Rif1\u306f\u30b2\u30ce\u30e0\u4e0a\u306b\u5b58\u5728\u3059\u308b\u8907\u6570\u306eG4\u69cb\u9020\u3068\u540c\u6642\u306b\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u3092\u5f62\u6210\u3057\u3001\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u9020\u5f62\u6210\u306e\u57fa\u76e4\u3068\u306a\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"2-Regulation-of-chromatin-structure-by-Rif1-through-its-binding-to-G4-at-nuclear-periphery\">\u6700\u65b0\u7814\u7a76\u6210\u679c(2) Regulation of chromatin structure by Rif1 through its binding to G4 at nuclear periphery<\/span><\/h2>\n<p><strong>Article in Molecular and Cellular Biology\u00a039(4). pii: e00364-18 (2019) \u00a0Selected for cover figure<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Both a Unique Motif at the C Terminus and an N-Terminal HEAT Repeat Contribute to G-Quadruplex Binding and Origin Regulation by the Rif1 Protein. <\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Kobayashi S, Fukatsu R, Kanoh Y, Kakusho N, Matsumoto S, Chaen S, and Masai H.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>Rif1\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u9175\u6bcd\u304b\u3089\u30d2\u30c8\u307e\u3067\u9032\u5316\u7684\u306b\u4fdd\u5b58\u3055\u308c\u3001\u67d3\u8272\u4f53DNA\u8907\u88fd\u958b\u59cb\u306e\u6642\u9593\u7684\u30bf\u30a4\u30df\u30f3\u30b0\u3068\u3001\u6838\u5185\u3067\u306e\u7a7a\u9593\u7684\u914d\u7f6e\u3092\u5236\u5fa1\u3059\u308b\u3002\u5f53\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3067\u306f\u4ee5\u524d\u3001Rif1\u306f\u4ee3\u8868\u7684\u306a\u975eB\u578bDNA\u69cb\u9020\u3067\u3042\u308b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3092\u5831\u544a\u3057\u305f(Nature Structural and Molecular Biology 2015; J Biol Chem 2018)\u3002\u4eca\u56de\u3001\u5206\u88c2\u9175\u6bcd\u306eRif1\u306e\u8a73\u7d30\u306a\u5909\u7570\u4f53\u306e\u751f\u5316\u5b66\u7684\u3001\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u306b\u3088\u308a\u3001Rif1\u306eC\u7aef\u30e6\u30cb\u30fc\u30af\u30e2\u30c1\u30fc\u30d5\u304a\u3088\u3073N\u7aef\u306eHEAT\u30ea\u30d4\u30fc\u30c8\u69cb\u9020\u306e\u4e21\u8005\u304c\u72ec\u7acb\u306bG4\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3001Rif1\u306f12~16\u91cf\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3001C\u7aef91\u30a2\u30df\u30ce\u9178\u306e\u307f\u3067\u591a\u91cf\u4f53\u5f62\u6210\u3067\u304d\u308b\u3053\u3068\u3001\u4e21\u30e2\u30c1\u30fc\u30d5\u304c\u3001Rif1\u306b\u3088\u308b\u8907\u88fd\u8d77\u70b9\u6291\u5236\u6d3b\u6027\u306b\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u5909\u7570\u4f53\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u304b\u3089\u3001\u8907\u88fd\u8d77\u70b9\u6291\u5236\u80fd\u3092\u55aa\u5931\u3057\u305f\u65b0\u898f\u5909\u7570\u4f53\u3092\u5358\u96e2\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u4e2d\u306b\u306f\u30af\u30ed\u30de\u30c1\u30f3\u7d50\u5408\u80fd\u3092\u55aa\u5931\u3057\u3066\u3044\u308b\u3082\u306e\u3068\u3001\u4fdd\u6301\u3057\u3066\u3044\u308b\u3082\u306e\u304c\u5b58\u5728\u3057\u305f\u3002\u751f\u5316\u5b66\u7684\u89e3\u6790\u306e\u7d50\u679c\u306b\u3082\u3068\u3065\u304d\u3001Rif1\u306b\u3088\u308b\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u5f62\u6210\u3068\u3001\u305d\u308c\u306b\u3088\u308b\u8907\u88fd\u958b\u59cb\u3092\u6291\u5236\u3059\u308b\u30af\u30ed\u30de\u30c1\u30f3\u69cb\u9020\u306e\u69cb\u7bc9\u306e\u30e2\u30c7\u30eb\u3092\u63d0\u5531\u3057\u3001\u8868\u7d19\u306b\u63a1\u7528\u3055\u308c\u305f\uff08\u56f3\u53c2\u7167\uff09\u3002\u3053\u306e\u7814\u7a76\u306b\u306f\u3001\u4e3b\u306b\u3001\u5f53\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u306e\u4fee\u58eb\uff12\u5e74\u306e\u5c0f\u6797\u99ff\u4ecb\u6c0f\u3001\u7814\u7a76\u6280\u8853\u54e1\u306e\u6df1\u6d25\u7406\u4e43\u6c0f\u3001\u304a\u3088\u3073\u7814\u7a76\u54e1\u306e\u52a0\u7d0d\u8c4a\u535a\u58eb\u306b\u3088\u308a\u884c\u308f\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cover3_1.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-large wp-image-2549 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cover3_1-471x620.jpg\" alt=\"cover3_1\" width=\"471\" height=\"620\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cover3_1-471x620.jpg 471w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cover3_1-228x300.jpg 228w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cover3_1.jpg 1264w\" sizes=\"(max-width: 471px) 100vw, 471px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u56f3 Rif1\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u308b\u30af\u30ed\u30de\u30c1\u30f3\u9ad8\u6b21\u69cb\u9020\u5f62\u6210\u306e\u30e2\u30c7\u30eb<\/strong><\/p>\n<p>Rif1\u306fC\u7aefG4\u7d50\u5408\u30c9\u30e1\u30a4\u30f3(\u8d64)\u3068N\u7aefHEAT\u30ea\u30d4\u30fc\u30c8\u69cb\u9020(\u9ec4\u7dd1)\u304b\u3089\u5f62\u6210\u3055\u308c\u308b\u3002C\u7aef\u306f8~16\u91cf\u4f53\u3092\u5f62\u6210\u3059\u308b\u3068\u3068\u3082\u306bG4\u69cb\u9020\u306b\u7d50\u5408\u3059\u308b\u3002HEAT \u30ea\u30d4\u30fc\u30c8\u3082\u4f1a\u5408\u3059\u308b\u3068\u3068\u3082\u306b\u3001G4\u69cb\u9020\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u3002Rif1\u306f\u3001\u540c\u6642\u306b\u8907\u6570\u306eG4\u69cb\u9020\u3068\u76f8\u4e92\u4f5c\u7528\u3067\u304d\u308b\u3002\u307e\u305f\u96fb\u5b50\u9855\u5fae\u93e1\u3067\u306e\u89b3\u5bdf\u306a\u3069\u306b\u57fa\u3065\u304d\u3001Rif1\u306fN\u7aef\u3068C\u7aef\u306e\u4e21\u30c9\u30e1\u30a4\u30f3\u3092\u4ecb\u3057\u3066\u30b2\u30ce\u30e0\u4e0a\u306b\u6563\u5728\u3059\u308bG4\u69cb\u9020\u306b\u7d50\u5408\u3057\u3001\u3053\u308c\u3089\u3092\u675f\u306d\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u3092\u5f62\u6210\u3059\u308b\u3002Rif1\u306f\u6838\u819c\u3068\u76f8\u4e92\u4f5c\u7528\u3057\u3001\u6838\u819c\u8fd1\u508d\u306b\u5c40\u5728\u3057\u3066\u304a\u308a\u3001\u6838\u819c\u306e\u8fd1\u508d\u3067\u3053\u306e\u3088\u3046\u306a\u69cb\u9020\u3092\u4f5c\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002\u3053\u306e\u69cb\u9020\u306f\u3001TAD\u3084LAD\u3068\u3082\u95a2\u9023\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u8907\u88fd\u958b\u59cb\u306b\u6291\u5236\u7684\u306a\u30af\u30ed\u30de\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\uff08\u4e2d\u671f~\u5f8c\u671f\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30c9\u30e1\u30a4\u30f3\uff09\u3092\u5f62\u6210\u3059\u308b\u3068\u60f3\u50cf\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"3-Structures-of-DNA-containing-G4-forming-sequences-on-duplex-DNA\">\u6700\u65b0\u7814\u7a76\u6210\u679c(3) Structures of DNA containing G4-forming sequences on duplex DNA<\/span><\/h2>\n<p><strong>Article in\u00a0Journal of Biological Chemistry 293(44):17033-17049 (2018)<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a <strong>Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences.\u00a0<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Masai H, Kakusho N, Fukatsu R, Ma Y, Iida K, Kanoh Y, and Nagasawa K.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\u4e8c\u672c\u9396DNA\u4e0a\u306eRif1\u7d50\u5408\u90e8\u4f4d\u304c\u5f62\u6210\u3059\u308b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u3092\u542b\u3080DNA\u9ad8\u6b21\u69cb\u9020\u3092\u8a73\u7d30\u306b\u89e3\u6790\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001G-rich\u9396\u306bG4\u69cb\u9020\u304c\u5f62\u6210\u3055\u308c\u308b\u3068\u3068\u3082\u306b\u3001\u9006\u9396\u306b\u3082S1\u611f\u53d7\u6027\u306e\u69cb\u9020\u304c\u5f62\u6210\u3055\u308c\u308b\u3002\u3053\u306e\u69cb\u9020\u306f\u3001G-rich\u9396\u4e0a\u306eG4\u5f62\u6210\u306b\u4f9d\u5b58\u3057\u3066\u304a\u308a\u3001i-motif\u306e\u5f62\u6210\u3092\u793a\u3059\u8a3c\u62e0\u306f\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/0a661645f31d832e95a4e372ca27720d.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-full wp-image-2588 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/0a661645f31d832e95a4e372ca27720d.jpg\" alt=\"masai JBC\u306e\u56f3\" width=\"2023\" height=\"1440\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/0a661645f31d832e95a4e372ca27720d.jpg 2023w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/0a661645f31d832e95a4e372ca27720d-300x214.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/0a661645f31d832e95a4e372ca27720d-620x441.jpg 620w\" sizes=\"(max-width: 2023px) 100vw, 2023px\" \/><\/a>\u56f3\u3000\u4e00\u672c\u9396Rif1BS DNA\uff08\u91ce\u751f\u578b\u3068\u3001G4\u5f62\u6210\u306b\u5f71\u97ff\u3092\u3042\u305f\u3048\u308b\u5909\u7570\u4f53\uff09\u3092\u71b1\u51e6\u7406\u3057\u305f\u5f8c\u306b\u3001S1\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3084DMS\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u3092\u89e3\u6790\u3057\u305f\u3002G-tracts\u306e\u5909\u7570\u306b\u3088\u308a\u3001\u5225\u306eG-traact\u3092\u7528\u3044\u3066\u3053\u3068\u306a\u308b\u3001G4\u69cb\u9020\u304c\u5f62\u6210\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u793a\u3055\u308c\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"4-Biochemical-characterization-of-mammalian-Rif1-protein\">\u6700\u65b0\u7814\u7a76\u6210\u679c(4) Biochemical characterization of mammalian Rif1 protein<\/span><\/h2>\n<p><strong>Article in\u00a0Journal of Biological Chemistry 293(10):3607-3624<\/strong><strong>\u00a0(2018)<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a\u00a0<strong>Oligomer formation and G-quadruplex binding by purified murine Rif1 protein, a key organizer of higher-order chromatin architecture.<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Moriyama K, Yoshizawa-Sugata N, and Masai H.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\u30de\u30a6\u30b9Rif1\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7cbe\u88fd\u3057\u3001\u305d\u306eDNA\u7d50\u5408\u80fd\u306a\u3069\u306b\u3064\u3044\u3066\u8a73\u7d30\u306b\u89e3\u6790\u3057\u305f\u3002C\u7aef\u304a\u3088\u3073N\u7aefHEAT \u30ea\u30d4\u30fc\u30c8\u306e\u4e21\u8005\u306bG4\u7d50\u5408\u80fd\u529b\u3092\u6709\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3002\u307e\u305f\u3001Rif1\u306f\u591a\u91cf\u4f53\u3092\u5f62\u6210\u3057\u3001\u8907\u6570\u306eG4\u69cb\u9020\u306b\u540c\u6642\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3092\u3057\u3081\u3057\u305f\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/55a0ef6c2770aeeff22aaa0066db74b2.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-full wp-image-2586 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/55a0ef6c2770aeeff22aaa0066db74b2.jpg\" alt=\"muRif1\u30c9\u30e1\u30a4\u30f3\" width=\"587\" height=\"122\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/55a0ef6c2770aeeff22aaa0066db74b2.jpg 587w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/55a0ef6c2770aeeff22aaa0066db74b2-300x62.jpg 300w\" sizes=\"(max-width: 587px) 100vw, 587px\" \/><\/a><\/p>\n<p>\u56f3\uff11\u3000\u30de\u30a6\u30b9Rif1\u30bf\u30f3\u30d1\u30af\u8cea\u306eG4\u7d50\u5408\u30c9\u30e1\u30a4\u30f3<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/b233e2dc1060c94e14874c413d4b7c94.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2585 size-large alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/b233e2dc1060c94e14874c413d4b7c94-620x364.jpg\" alt=\"\u68ee\u5c71\u30e2\u30c7\u30eb\" width=\"620\" height=\"364\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/b233e2dc1060c94e14874c413d4b7c94-620x364.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/b233e2dc1060c94e14874c413d4b7c94-300x176.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/b233e2dc1060c94e14874c413d4b7c94.jpg 1147w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u56f3\uff12\u3000\u52d5\u7269\u7d30\u80deRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u591a\u91cf\u4f53\u5316\u306e\u30e2\u30c7\u30eb<\/p>\n<p>\u7dd1\u306fN\u7aefHEAT\u30c9\u30e1\u30a4\u30f3\u9818\u57df\u3001\u9752\u306fC\u7aef\u306eG4\u7d50\u5408\u30c9\u30e1\u30a4\u30f3\u3001\u30d4\u30f3\u30af\u306fIDP(\u5929\u7136\u30bf\u30f3\u30d1\u30af\u8cea\u9818\u57df)\u3092\u793a\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"5-DNA-strand-annealing-activities-of-Mcm-complexes\">\u6700\u65b0\u7814\u7a76\u6210\u679c(5) DNA strand annealing activities of Mcm complexes<\/span><\/h2>\n<p><strong>Article in\u00a0Nucleic Acids Research\u00a045(11):6494-6506 (2017)<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Potent DNA strand annealing activity associated with mouse Mcm2\u223c7 heterohexameric complex. <\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>You Z and Masai H.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\u771f\u6838\u751f\u7269DNA\u8907\u88fd\u3067\u3001\u4e8c\u672c\u9396DNA\u304c\u307b\u3069\u3051\u3066\u3086\u304f\u53cd\u5fdc\u306f\u3001\u3082\u3063\u3068\u3082\u91cd\u8981\u306a\u904e\u7a0b\u306e\u4e00\u3064\u3067\u3059\u3002\u3053\u306e\u53cd\u5fdc\u306b\u306f\u3001DNA\u30d8\u30ea\u30ab\u30fc\u30bc\u3068\u3044\u3046\u9175\u7d20\u304c\u91cd\u8981\u306a\u5f79\u5272\u3092\u306f\u305f\u3057\u307e\u3059\u3002Mcm2\u301c7\u516d\u91cf\u4f53\u8907\u5408\u4f53\u306f\u3001DNA\u8907\u88fd\u904e\u7a0b\u306b\u5fc5\u9808\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u751f\u7269\u7a2e\u306b\u4fdd\u5b58\u3055\u308c\u305f\u30d8\u30ea\u30ab\u30fc\u30bc\u306e\u4e3b\u8981\u306a\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306f\u3001MCM\u8907\u5408\u4f53\u306e\u4e00\u90e8\u3067\u3042\u308bMcm4\/6\/7\u8907\u5408\u4f53\u304cDNA\u30d8\u30ea\u30ab\u30fc\u30bc\u6d3b\u6027\u3092\u6301\u3064\u3053\u3068\u3092\u4e16\u754c\u306b\u5148\u99c6\u3051\u3066\u767a\u898b\u3057\u5831\u544a\u3057\u307e\u3057\u305f(You <em>et al<\/em>. 1999)\u3002\u3068\u3053\u308d\u304c\u3001\u7d30\u80de\u5185\u3067\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u679c\u305f\u3059\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u308bMcm2\u301c7\u516d\u91cf\u4f53\u8907\u5408\u4f53\u306f\u3001\u3053\u308c\u307e\u3067\u901a\u5e38\u306e\u6761\u4ef6\u4e0b\u3067\u306f\u30d8\u30ea\u30ab\u30fc\u30bc\u306e\u6d3b\u6027\u3092\u793a\u3055\u306a\u3044\u3053\u3068\u304b\u3089\u3001\u672a\u77e5\u306e\u5236\u5fa1\u6a5f\u69cb\u304c\u3042\u308b\u3068\u63a8\u6e2c\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u306e\u5f8c\u3001Mcm2~7\u516d\u91cf\u4f53\u8907\u5408\u4f53\u304c\u3001\u4ed6\u306e\u8907\u88fd\u56e0\u5b50\u3067\u3042\u308b\u3001Cdc45\u304a\u3088\u3073GINS\u3068\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u521d\u3081\u3066\u6d3b\u6027\u306e\u3042\u308bDNA\u30d8\u30ea\u30ab\u30fc\u30bc\u3068\u306a\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u4f9d\u7136\u3068\u3057\u3066\u3001\u306a\u305cMcm2\u301c7\u516d\u91cf\u4f53\u8907\u5408\u4f53\u304c\u30d8\u30ea\u30ab\u30fc\u30bc\u6d3b\u6027\u3092\u793a\u3055\u306a\u3044\u304b\u306f\u4e0d\u660e\u3067\u3057\u305f\u3002<\/p>\n<p>\u4eca\u56de\u3001\u79c1\u305f\u3061\u306f\u7cbe\u88fd\u3055\u308c\u305f\u30de\u30a6\u30b9Mcm2\u301c7\u304c\u3001DNA\u30d8\u30ea\u30ab\u30fc\u30bc\u306e\u9006\u53cd\u5fdc\u3067\u3042\u308b\u3001\u4e8c\u672c\u306e\u76f8\u88dc\u7684\u306a\uff11\u672c\u9396DNA\u3092\u4f1a\u5408\u3055\u305b\u3066\u3001\u4e8c\u672c\u9396DNA\u3092\u5f62\u6210\u3059\u308b\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0(\u76f8\u88dc\u5bfe\u5f62\u6210)\u6d3b\u6027\u3092\u6301\u3064\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u30d8\u30ea\u30ab\u30fc\u30bc\u6d3b\u6027\u306b\u3088\u308a\u306f\u304c\u3055\u308c\u305f \u4e00\u672c\u9396DNA\u304c\u3001\u76f8\u88dc\u9396\u5f62\u6210\u3059\u308b\u3053\u3068\u3092\u963b\u5bb3\u3059\u308b\u6761\u4ef6\u3067\u306f\u3001Mcm2\u301c7\u306f\u52b9\u7387\u306e\u3088\u3044\u30d8\u30ea\u30ab\u30fc\u30bc\u6d3b\u6027\u3092\u793a\u3059\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089Mcm2\u301c7\u306f\u5358\u72ec\u3067\u30d8\u30ea\u30ab\u30fc\u30bc\u6d3b\u6027\u3092\u6709\u3059\u308b\u304c\u3001\u9006\u53cd\u5fdc\u306e\u6d3b\u6027\u306e\u305f\u3081\u306b\u3053\u308c\u307e\u3067\u691c\u51fa\u3055\u308c\u3066\u3044\u306a\u304b\u3063\u305f\u53ef\u80fd\u6027\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u76f8\u88dc\u9396\u5f62\u6210\u53cd\u5fdc\u306f\u3001DNA\u640d\u50b7\u306a\u3069\u306b\u3088\u308a\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u304c\u505c\u6b62\u3057\u305f\u6642\u306b\u751f\u3058\u308b\u4e00\u672c\u9396DNA\u3092\u4e8c\u672c\u9396\u3078\u3068\u623b\u3059\u3053\u3068\u306b\u3088\u308a\u3001\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u304c\u58ca\u308c\u3066\u3057\u307e\u308f\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u91cd\u8981\u306a\u5f79\u5272\u304c\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u4eca\u56de\u306e\u767a\u898b\u306f\u3001Mcm\u304c\u8907\u88fd\u306e\u904e\u7a0b\u3067\u4e8c\u672c\u9396DNA\u3092\u307b\u3069\u3044\u3066\u3086\u304f\u306e\u307f\u3067\u306a\u304f\u3001\u304c\u3093\u306a\u3069\u306e\u75be\u60a3\u306e\u539f\u56e0\u3068\u306a\u308b\u3001\u30b2\u30ce\u30e0\u306e\u5d29\u58ca\u3092\u9632\u3050\u3068\u3044\u3046\u91cd\u8981\u306a\u50cd\u304d\u3092\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/c52c205879afa52da00a9524f3abb73c.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-full wp-image-2567 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/c52c205879afa52da00a9524f3abb73c.jpg\" alt=\"\u30c8\u30ea\u30df\u30f3\u30b0\u3000\u9ad8\u4e95\u3000\u533b\u5b66\u7814\u30cb\u30e5\u30fc\u30b9\u56f3\" width=\"613\" height=\"324\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/c52c205879afa52da00a9524f3abb73c.jpg 613w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/c52c205879afa52da00a9524f3abb73c-300x159.jpg 300w\" sizes=\"(max-width: 613px) 100vw, 613px\" \/><\/a><\/p>\n<p>\u56f3\u3000Mcm\u8907\u5408\u4f53\u306f\u3001\u5f37\u3044\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6d3b\u6027\u3092\u6709\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u3053\u306e\u305f\u3081\u306b\u3001\u901a\u5e38\u306e\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u5dfb\u304d\u623b\u3057\u6d3b\u6027\u304c\u691c\u51fa\u3055\u308c\u306b\u304f\u304b\u3063\u305f\u3002\u4e00\u65b9\u3001CMG\u30d8\u30ea\u30ab\u30fc\u30bc\u306f\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u6d3b\u6027\u304c\u6291\u5236\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u5dfb\u304d\u623b\u3057\u6d3b\u6027\u304c\u691c\u51fa\u3055\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"6-Negative-regulation-of-origin-firing-by-Mrc1\">\u6700\u65b0\u7814\u7a76\u6210\u679c(6) Negative regulation of origin firing by Mrc1<\/span><\/h2>\n<p><strong>Article in Molecular and Cellular Biology 37(7). pii: e00355-16. \u00a0(2017) \u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Checkpoint-Independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 Kinase. <\/strong><\/p>\n<p><span style=\"font-size: 14.079999923706055px;\">\u8457\u8005\uff1a<\/span><span style=\"font-size: 14.079999923706055px;\"><strong>Matsumoto S, Kanoh Y, Shimmoto M, Hayano M, Ueda K, Fukatsu R, Kakusho N, and Masai H.<\/strong>\u00a0<\/span><\/p>\n<p><strong style=\"font-size: 14.079999923706055px;\">\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>Mrc1\u306f\u8907\u88fd\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u306e\u30e1\u30c7\u30a3\u30a8\u30a4\u30bf\u30fc\u3068\u3057\u3066\u5358\u96e2\u3055\u308c\u3001\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u6a5f\u80fd\u306b\u4f9d\u5b58\u3057\u3066\u5f8c\u671f\u8907\u88fd\u8d77\u70b9\u304b\u3089\u306e\u8907\u88fd\u958b\u59cb\u3092\u5236\u5fa1(\u6291\u5236)\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u79c1\u305f\u3061\u306f\u3001Mrc1\u306f\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u975e\u4f9d\u5b58\u7684\u306b\u521d\u671f\u8907\u88fd\u8d77\u70b9\u304b\u3089\u306e\u8907\u88fd\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u4ee5\u524d\u306b\u5831\u544a\u3057\u305f(Hayano et al. [2011]; Matsumoto et al. [2011])\u304c\u3001\u305d\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u4e0d\u660e\u3067\u3042\u3063\u305f\u3002\u4eca\u56de\u79c1\u305f\u3061\u306fMrc1\u306f\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u3092\u4ecb\u3057\u3066\u305d\u306e\u5206\u5b50\u5f62\u614b\u3092\u5909\u5316\u3055\u305b\u3001\u8907\u88fd\u958b\u59cb\u306e\u30d6\u30ec\u30fc\u30ad\u306eon\/off\u3092\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u306b\u95a2\u4e0e\u3059\u308bHBS\u3068\u540d\u4ed8\u3051\u305f\u9818\u57df\u306b\u306f\u5206\u88c2\u9175\u6bcdCdc7\u30ad\u30ca\u30fc\u30bc\u30db\u30e2\u30ed\u30b0\u3067\u3042\u308bCdc7\u304c\u7d50\u5408\u3057\u3001Mrc1\u306eN\u7aef\u9818\u57df\u306e\u30ea\u30f3\u9178\u5316\u3092\u4ecb\u3057\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u9664\u3057Mrc1\u306e\u30d6\u30ec\u30fc\u30ad\u3092\u89e3\u9664\u3055\u305b\u308b\u3002\u3053\u306eMrc1\u306e\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u3068\u305d\u308c\u306b\u3088\u308b\u5206\u5b50\u5f62\u614b\u5909\u5316\u3092\u4ecb\u3057\u305f\u30d6\u30ec\u30fc\u30ad\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u3001\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u975e\u4f9d\u5b58\u7684\u306a\u521d\u671f\u8907\u88fd\u8d77\u70b9\u306e\u9078\u629e\u3068\u8907\u88fd\u958b\u59cb\u30bf\u30a4\u30df\u30f3\u30b0\u306e\u5236\u5fa1\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/MCB-Mrc1-model.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" size-large wp-image-2550 alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/MCB-Mrc1-model-620x395.jpg\" alt=\"MCB Mrc1 model\" width=\"620\" height=\"395\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/MCB-Mrc1-model-620x395.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/MCB-Mrc1-model-300x191.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/MCB-Mrc1-model.jpg 1254w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p><strong>\u56f3\u3000\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u308bconformaton\u5909\u63db\u306b\u3088\u308bMrc1\u6d3b\u6027\u306e\u5236\u5fa1<\/strong><\/p>\n<p>Mrc1\u306f\u3001\u901a\u5e38\u3001\u8907\u88fd\u958b\u59cb\u3092\u6291\u5236\u3059\u308b\u30d6\u30ec\u30fc\u30adon\u306e\u5f62\u614b\u3092\u53d6\u3063\u3066\u3044\u308b(\u8907\u88fd\u958b\u59cb\u306bhsk1\u6a5f\u80fd\u3092\u5fc5\u8981\u3068\u3059\u308b)\u3002\u3053\u306e\u6642\u3001Mrc1\u5206\u5b50\u4e0a\u306eHBS(Hsk1 binding sequence)\u306fN\u7aef\u8fd1\u508d\u306eNTHBS\u3068\u5206\u5b50\u5185\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u3044\u308b\u3002Cdc7\u30ad\u30ca\u30fc\u30bc(\u5206\u88c2\u9175\u6bcd\u3067\u306fHsk1\u30ad\u30ca\u30fc\u30bc\u3068\u547c\u3070\u308c\u308b)\u306fHBS\u3092\u4ecb\u3057\u3066Mrc1\u306b\u7d50\u5408\u3057\u3001NTHBS\u306e\u8fd1\u508d\u3092\u30ea\u30f3\u9178\u5316\u3059\u308b\u3002\u305d\u306e\u7d50\u679c\u3001HBS-NTHBS\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u89e3\u9664\u3055\u308c\u308b\u3002\u305d\u306e\u7d50\u679c\u3001Mrc1\u306econformation\u304c\u5909\u5316\u3057\u3001\u30d6\u30ec\u30fc\u30adoff\u306e\u5f62\u614b\u306b\u306a\u308b(\u53f3\u4e0b;\u8907\u88fd\u958b\u59cb)\u3002\u2206HBS\u306fHBS\u306e\u6b20\u640d\u306e\u305f\u3081\u306b\u3001\u6052\u5e38\u7684\u306b\u30d6\u30ec\u30fc\u30adoff\u306econformation\u3092\u3068\u308b\u305f\u3081\u306b\u3001hsk1\u306a\u3057\u3067\u3082\u8907\u88fd\u958b\u59cb\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\uff08hsk1\u5909\u7570\u306e\u30d0\u30a4\u30d1\u30b9\uff09\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"7-Regulation-of-initiation-of-DNA-replication-by-Claspin\">\u6700\u65b0\u7814\u7a76\u6210\u679c(7) Regulation of initiation of DNA replication by Claspin<\/span><\/h2>\n<p><strong>Article in Nature Communications 7:12135. (<\/strong><strong>2017)<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells.\u00a0<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Yang CC, Suzuki M, Yamakawa S, Uno S, Ishii A, Yamazaki S, Fukatsu R, Fujisawa R, Sakimura K, Tsurimoto T, and Masai H.<\/strong><\/p>\n<p><strong>Review article in Current Genetics\u00a063(5):813-818.\u00a0<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Mrc1\/Claspin: a new role for regulation of origin firing.<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Masai H, Yang CC, and Matsumoto S.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\uff11\u3000\u7814\u7a76\u306e\u80cc\u666f<\/p>\n<p>\u30b2\u30ce\u30e0\u8907\u88fd\u306f\u7d30\u80de\u306e\u5897\u6b96\u306b\u3068\u3063\u3066\u6700\u3082\u57fa\u672c\u7684\u306a\u4e8b\u8c61\u3067\u3059\u3002\u30d2\u30c8\u7d30\u80de\u306f\u5206\u88c2\u3059\u308b\u3054\u3068\u306b\u300130\u5104\u5869\u57fa\u5bfe\u306eDNA\u3092\u307b\u3068\u3093\u3069\u8aa4\u308a\u306a\u304f\u30b3\u30d4\u30fc\u3057\u3001\u5168\u304f\u540c\u3058\u30b2\u30ce\u30e0DNA\u3092\u3082\u3046\uff11\u30bb\u30c3\u30c8\u4f5c\u308a\u4e0a\u3052\u307e\u3059\u3002\u4e00\u65b9\u3001\u8907\u88fd\u306e\u904e\u7a0b\u306f6-8\u6642\u9593\u304b\u304b\u308b\u9577\u3044\u904e\u7a0b\u3067\u3059\u304c\u3001\u305d\u306e\u9593\u306b\u969c\u5bb3\u3092\u53d7\u3051\u3066\u8907\u88fd\u304c\u6b62\u307e\u3063\u305f\u308a\u3001\u3042\u308b\u3044\u306f\u30b3\u30d4\u30fc\u306e\u9593\u9055\u3044\u3092\u6ca2\u5c71\u8d77\u3053\u3059\u3068\u3001\u305d\u308c\u306f\u30b2\u30ce\u30e0\u306e\u5909\u5316\u3068\u3057\u3066\u84c4\u7a4d\u3057\u3001\u3084\u304c\u3066\u304c\u3093\u3084\u305d\u306e\u4ed6\u306e\u75be\u60a3\u306e\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u8907\u88fd\u306e\u958b\u59cb\u30fb\u9032\u884c\u306f\u3001\u7d30\u80de\u5468\u671f\u3068\u53b3\u5bc6\u306b\u9023\u52d5\u3057\u3066\u304a\u308a\u3001\u305d\u306e\u9023\u52d5\u304c\u7834\u7dbb\u3057\u3066\u3082\u3001\u30b2\u30ce\u30e0\u306e\u5909\u5316\u304c\u8a98\u5c0e\u3055\u308c\u307e\u3059\u3002\u6b63\u5e38\u7d30\u80de\u306b\u304a\u3044\u3066\u306f\u3001\u30b2\u30ce\u30e0\u5909\u5316\u304c\u6700\u5c0f\u306b\u306a\u308b\u3088\u3046\u306b\u3001\u8907\u88fd\u306e\u904e\u7a0b\u306f\u53b3\u5bc6\u306b\u5236\u5fa1\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u304c\u3093\u7d30\u80de\u3067\u306f\u8907\u88fd\u306e\u5236\u5fa1\u306b\u969c\u5bb3\u304c\u751f\u3058\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30b2\u30ce\u30e0\u304c\u201d\u4e0d\u5b89\u5b9a\u201d\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u8a73\u7d30\u306f\u4e0d\u660e\u3067\u3059\u3002<\/p>\n<p>\uff12\u3000\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/p>\n<p>\u79c1\u305f\u3061\u306f\u8907\u88fd\u958b\u59cb\u306e\u5f15\u304d\u91d1\u3092\u5f15\u304fCdc7\u30ad\u30ca\u30fc\u30bc\u306e\u7814\u7a76\u3092\u9577\u5e74\u884c\u3063\u3066\u304d\u307e\u3057\u305f\u3002Cdc7\u306fMcm\u3068\u3044\u3046DNA\u4e8c\u672c\u9396\u3092\u958b\u88c2\u3059\u308b\u9175\u7d20\u3092\u30ea\u30f3\u9178\u5316\u3057\u8907\u88fd\u958b\u59cb\u3092\u958b\u59cb\u3055\u305b\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u5831\u544a\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001Claspin\u306f\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u306e\u300c\u898b\u5f35\u308a\u5f79\u300d\u3067\u3001\u8907\u88fd\u304c\u6b62\u307e\u3063\u305f\u308a\u3001\u52d5\u304d\u304c\u60aa\u304f\u306a\u3063\u305f\u3068\u304d\u306b\u305d\u308c\u3092\u898b\u3064\u3051\u3066\u4fee\u6b63\u3059\u308b\u5f79\u5272\u3092\u306f\u305f\u3057\u307e\u3059\u3002\u4eca\u56de\u3001\u79c1\u305f\u3061\u306fClaspin\u306f\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u304c\u5f62\u6210\u3055\u308c\u308b\u524d\u306bCdc7\u3092\u547c\u3073\u8fbc\u3080\u5f79\u5272\u3092\u3059\u308b\u3053\u3068\u3001\u3055\u3089\u306bClaspin\u306fCdc7\u306b\u3088\u308a\u30ea\u30f3\u9178\u5316\u3055\u308c\u3001\u305d\u306e\u5f62\u614b\u3092\u5909\u5316\u3055\u305bDNA\u3068PCNA\u306b\u7d50\u5408\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u306b\u3068\u308a\u3053\u307e\u308c\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u3055\u3089\u306b\u8208\u5473\u6df1\u3044\u3053\u3068\u306b\u3001\u304c\u3093\u7d30\u80de\u3067\u306fCdc7\u306fClaspin\u304c\u306a\u304f\u3066\u3082\u8907\u88fd\u958b\u59cb\u306b\u5fc5\u8981\u306a\u30ea\u30f3\u9178\u5316\u3092\u884c\u3046\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u306e\u6210\u679c\u304b\u3089Claspin\u306f\u3001Cdc7\u3068\u9023\u643a\u3057\u3066\u3001\u7d30\u80de\u5468\u671f\u9032\u884c\u3068\u9023\u52d5\u3057\u305f\u3001\u79e9\u5e8f\u6b63\u3057\u3044\u8907\u88fd\u958b\u59cb\u3068\u9032\u884c\u3092\u4fdd\u8a3c\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\uff08\u56f3\uff09\u3002\u4e00\u65b9\u3001\u304c\u3093\u7d30\u80de\u3067\u306f\u3053\u306e\u5236\u5fa1\u3092\u5931\u3046\u3053\u3068\u306b\u3088\u308a\u8907\u88fd\u306e\u958b\u59cb\u3068\u9032\u884c\u306e\u8131\u5236\u5fa1\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u3001\u7d30\u80de\u5468\u671f\u9032\u884c\u306e\u7570\u5e38\u3001\u30b2\u30ce\u30e0\u4e0d\u5b89\u5b9a\u6027\u4ea2\u9032\u304c\u3082\u305f\u3089\u3055\u308c\u307e\u3057\u3002<\/p>\n<p>\uff13\u3000\u767a\u898b\u306e\u610f\u7fa9<\/p>\n<p>Cdc7\u30ad\u30ca\u30fc\u30bc\u306f\u8907\u88fd\u958b\u59cb\u306e\u5f15\u304d\u91d1\u3092\u3072\u304f\u304c\u3001\u4eca\u56de\u306e\u767a\u898b\u306f\u3001Claspin\u306f\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u306e\u9032\u884c\u306e\u307f\u306a\u3089\u305a\u3001Cdc7\u30ad\u30ca\u30fc\u30bc\u3092\u547c\u3073\u8fbc\u3080\u4e8b\u306b\u3088\u308a\u8907\u88fd\u958b\u59cb\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3068\u90e8\u4f4d\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u767a\u898b\u306f\u52d5\u7269\u7d30\u80de\u306e\u30b2\u30ce\u30e0\u8907\u88fd\u5236\u5fa1\u306b\u65b0\u3057\u3044\u8996\u70b9\u3092\u52a0\u3048\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001\u3053\u306e\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0\u306f\u304c\u3093\u7d30\u80de\u3067\u306f\u4f5c\u52d5\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u3082\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u672c\u7814\u7a76\u306e\u767a\u898b\u306f\u3001\u304c\u3093\u7d30\u80de\u306e\u30b2\u30ce\u30e0\u4e0d\u5b89\u5b9a\u6027\u306e\u539f\u56e0\u3068\u306a\u308b\u8907\u88fd\u5236\u5fa1\u7570\u5e38\u306e\u4e00\u5074\u9762\u3092\u521d\u3081\u3066\u660e\u3089\u304b\u306b\u3057\u305f\u3068\u3044\u3046\u70b9\u3067\u3082\u610f\u7fa9\u6df1\u3044\u3082\u306e\u3067\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/81d65bdebe1f290cf479cbfe89cfd038.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-2568 size-full alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/81d65bdebe1f290cf479cbfe89cfd038.jpg\" alt=\"\u30ce\u30d0\u56f3\u6539\u5b9a\" width=\"585\" height=\"312\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/81d65bdebe1f290cf479cbfe89cfd038.jpg 585w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/81d65bdebe1f290cf479cbfe89cfd038-300x160.jpg 300w\" sizes=\"(max-width: 585px) 100vw, 585px\" \/><\/a><\/p>\n<p>\u56f3\u3000Claspin\u306e\u8907\u88fd\u958b\u59cb\u306b\u304a\u3051\u308b\u5f79\u5272\u3068\u304c\u3093\u7d30\u80de\u3068\u6b63\u5e38\u7d30\u80de\u306e\u9055\u3044<\/p>\n<p>\u6b63\u5e38\u7d30\u80de\uff1aClaspin\u304c\u7d50\u5408\u3059\u308bori\u306b\u304a\u3044\u3066acidic patch\u306bCdc7\u304c\u547c\u3073\u8fbc\u307e\u308c\u3001Mcm\u306e\u30ea\u30f3\u9178\u5316\u3092\u3072\u304d\u304a\u3053\u3059\u3002\u304c\u3093\u7d30\u80de\uff1bClaspin\u304c\u306a\u304f\u3066\u3082\u904e\u5270\u306b\u5b58\u5728\u3059\u308bCdc7\u306b\u3088\u308aMcm\u306f\u30ea\u30f3\u9178\u5316\u3055\u308c\u8907\u88fd\u304c\u8131\u5236\u5fa1\u3055\u308c\u305f\u72b6\u614b\u3067\u958b\u59cb\u3059\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<h2><span id=\"8-Rif1-binds-regulates-replication-timing-through-binding-to-G-quadruplex-structures-on-the-genome\">\u6700\u65b0\u7814\u7a76\u6210\u679c(8) Rif1 binds regulates replication timing through binding to G-quadruplex structures on the genome.<\/span><\/h2>\n<p><strong>Article in Nature Structural &amp; Molecular Biology 2<\/strong><b>2: 889-897<\/b><strong>\u00a0(2015)<\/strong><\/p>\n<p>\u8ad6\u6587\u984c\u76ee\uff1a<strong>Rif1 binds to G-quadruplex and suppresses replication over a long distances.<\/strong><\/p>\n<p>\u8457\u8005\uff1a<strong>Kanoh Y, Matsumoto S, Fukatsu R, Kakusho N, Kono N, Renard-Guillet C, Masuda K, Iida K, Nagasawa K, Shirahige K, and Masai H.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7814\u7a76\u6210\u679c\u306e\u6982\u8981<\/strong><\/p>\n<p>\u30b2\u30ce\u30e0\u306e\u4e2d\u306b\u306f\u901a\u5e38\u306eB\u578b\u4e8c\u91cd\u3089\u305b\u3093DNA\u69cb\u9020\u4ee5\u5916\u306b\u591a\u304f\u306eDNA\u7279\u6b8a\u9ad8\u6b21\u69cb\u9020\u304c\u5b58\u5728\u3059\u308b\u3002\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u306f\u305d\u306e\u4e2d\u3067\u3082\u7279\u306b\u591a\u304f\u5b58\u5728\u3059\u308b\uff08\u5927\u8178\u83cc\u30b2\u30ce\u30e0\u306b6000\u7b87\u6240\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u306b35\u4e07\u7b87\u6240\u4ee5\u4e0a\uff09\u3002\u4eca\u56de\u3001\u30b2\u30ce\u30e0\u8907\u88fd\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u5236\u5fa1\u3059\u308b\u56e0\u5b50,Rif1\u304c\u3001\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3059\u308b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u30b2\u30ce\u30e0\u306e\u79e9\u5e8f\u6b63\u3057\u3044\u8907\u88fd\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u3053\u306e\u56e0\u5b50\u306f\u3001\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u3078\u306e\u7d50\u5408\u3092\u4ecb\u3057\u3066\u67d3\u8272\u4f53\u306e\u30c9\u30e1\u30a4\u30f3\u5f62\u6210\u306b\u95a2\u4e0e\u3057\u3001\u8907\u88fd\u3084\u8ee2\u5199\u306a\u3069\u67d3\u8272\u4f53\u306e\u591a\u304f\u306e\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002\u672c\u767a\u898b\u306f\u3053\u308c\u307e\u3067\u6ce8\u76ee\u3055\u308c\u3066\u3044\u306a\u304b\u3063\u305f\u30b2\u30ce\u30e0\u306eDNA\u7279\u6b8a\u69cb\u9020\u304c\u30b2\u30ce\u30e0\u7dad\u6301\u3001\u6a5f\u80fd\u767a\u73fe\u306b\u5927\u304d\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u4eca\u5f8c\u3001\u304c\u3093\u3084\u3001\u7a2e\u3005\u306e\u75be\u60a3\u306e\u539f\u56e0\u89e3\u660e\u306b\u3082\u65b0\u305f\u306a\u30d6\u30ec\u30fc\u30af\u30b9\u30eb\u30fc\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u308b\u3002<\/p>\n<p><strong>\u00a0<\/strong><strong>\u7814\u7a76\u306e\u80cc\u666f\uff1a<\/strong><\/p>\n<p>\u79c1\u305f\u3061\u306f\u3001\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u6c7a\u5b9a\u3059\u308b\u67d3\u8272\u4f53\u30c9\u30e1\u30a4\u30f3\u3092\u898f\u5b9a\u3059\u308b\u56e0\u5b50\u306e\u63a2\u7d22\u304b\u3089Rif1\u3092\u540c\u5b9a\u3057\u305f\u3002Rif1\u306f\u3001\u9175\u6bcd\u304b\u3089\u30d2\u30c8\u307e\u3067\u4fdd\u5b58\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u306e\u6b20\u640d\u306f\u3044\u305a\u308c\u306e\u7d30\u80de\u306b\u304a\u3044\u3066\u3082\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u306e\u5236\u5fa1\u306e\u55aa\u5931\u3092\u3082\u305f\u3089\u3059\u3002Rif1\u306f\u3001\u6838\u819c\u8fd1\u508d\u306e\u6838\u9aa8\u683c\u69cb\u9020\u306b\u304a\u3044\u3066\u67d3\u8272\u4f53\u306b\u7d50\u5408\u3057\u3001\u67d3\u8272\u4f53\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u5236\u5fa1\u3059\u308b\u53ef\u80fd\u6027\u3092\u6307\u6458\u3057\u305f\uff08\u56f31\uff09\u3002\u3055\u3089\u306bRif1\u6b20\u640d\u7d30\u80de\u306e\u89e3\u6790\u304b\u3089Rif1\u6b20\u640d\u306f\u3001\u8ee2\u5199\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u5909\u52d5\uff08\u8131\u6291\u5236\uff09\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u3092\u898b\u51fa\u3057\u305f\u3002\u307e\u305f\u3001Rif1\u306fDNA\u5207\u65ad\u4fee\u5fa9\u904e\u7a0b\u306b\u3082\u95a2\u4e0e\u3059\u308b\u3002\u3053\u306e\u3088\u3046\u306bRif1\u306f\u3001\u67d3\u8272\u4f53\u306b\u7d50\u5408\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u306e\u5f62\u6210\u3092\u4ecb\u3057\u3066\u3001\u67d3\u8272\u4f53\u306e\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u3092\u5236\u5fa1\u3059\u308b\u4e8b\u306b\u3088\u308a\u8907\u88fd\u30fb\u8ee2\u5199\u30fb\u4fee\u5fa9\u306a\u3069\u7a2e\u3005\u306e\u67d3\u8272\u4f53\u306e\u52d5\u614b\u3092\u5236\u5fa1\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002Rif1\u306f\u6838\u306b\u5b58\u5728\u3057\u3001\u67d3\u8272\u4f53\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u304c\u3001\u3069\u306e\u3088\u3046\u306b\u67d3\u8272\u4f53\u4e0a\u306b\u5c40\u5728\u3059\u308b\u304b\u306f\u77e5\u3089\u308c\u3066\u3044\u306a\u3044\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/3df738f6b6cea405ce2633acc6efb969.tif.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-1801\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/3df738f6b6cea405ce2633acc6efb969.tif-620x416.jpg\" alt=\"3df738f6b6cea405ce2633acc6efb969.tif\" width=\"620\" height=\"416\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/3df738f6b6cea405ce2633acc6efb969.tif-620x416.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/3df738f6b6cea405ce2633acc6efb969.tif-300x201.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/3df738f6b6cea405ce2633acc6efb969.tif.jpg 678w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p><strong>\u56f31 Rif1\u306f\u6838\u819c\u4e0a\u306e\u6838\u9aa8\u683c\u7d50\u5408\u9818\u57df\u306b\u7d50\u5408\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u69cb\u9020\u306e\u5f62\u6210\u3092\u4ecb\u3057\u3066\u4e2d\u671f\u30fb\u5f8c\u671f\u8907\u88fd\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3002<\/strong><\/p>\n<p>\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u306e\u7814\u7a76\u306b\u3064\u3044\u3066\u306f\u30d5\u30ed\u30ea\u30c0\u5dde\u7acb\u5927\u5b66\u306eDave Gilbert\u3089\u304c\u5148\u99c6\u7684\u306a\u7814\u7a76\u3092\u3057\u3066\u304a\u308a\u3001\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30c9\u30e1\u30a4\u30f3\u306f\u3001TAD(\u67d3\u8272\u4f53\u306e\u30c8\u30dd\u30ed\u30b8\u30fc\u306b\u95a2\u9023\u3057\u305f\u30c9\u30e1\u30a4\u30f3\u69cb\u9020)\u306b\u985e\u4f3c\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u308b(Nature 2015)\u3002\u53c8\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u306e\u6c7a\u5b9a\u306fG1\u671f\u306b\u306a\u3055\u308c\u308b\u3053\u3068\u3092Gilbert\u306f\u5831\u544a\u3057\u305f\u304c\u3001\u305d\u308c\u3092\u4f55\u304c\u5236\u5fa1\u3057\u3066\u3044\u308b\u304b\u306f\u660e\u3089\u304b\u3067\u306a\u304b\u3063\u305f\u3002\u6211\u3005\u306e\u767a\u898b\u304b\u3089Rif1\u304c\u305d\u306e\u4e3b\u8981\u306a\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002Rif1\u304c\u3001NHEJ(\u975e\u76f8\u540c\u672b\u7aef\u9023\u7d50)\u306b\u3088\u308bDSB(\u4e8c\u91cd\u9396DNA\u5207\u65ad)\u4fee\u5fa9\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u306f\u3001\u56fd\u5916\u306e\u8907\u6570\u306e\u30b0\u30eb\u30fc\u30d7\u304c\u767a\u898b\u3057\u5831\u544a\u3057\u305f(Cell, Mol Cell, Science\u306a\u3069;2013)\u3002\u307e\u305fRif1\u306e\u6b20\u640d\u306b\u3088\u308a\u8ee2\u5199\u304c\u5f71\u97ff\u3092\u53d7\u3051\u308b\u3053\u3068\u3082\u56fd\u5916\u306e\u30b0\u30eb\u30fc\u30d7\u306b\u3088\u308a\u5831\u544a\u3055\u308c\u305f\u3002Rif1\u306e\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u7d50\u5408\u90e8\u4f4d\u3068\u305d\u306e\u89e3\u6790\u306b\u3064\u3044\u3066\u306f\u4eca\u65e5\u307e\u3067\u7533\u8acb\u8005\u306e\u307f\u304c\u5831\u544a\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>G4\u69cb\u9020\u306b\u3064\u3044\u3066\u306f1980\u5e74\u4ee3\u5f8c\u534a\u306e\u767a\u898b\u4ee5\u6765\u3001\u591a\u304f\u306e\u305d\u306e\u5316\u5b66\u7684\u3001\u69cb\u9020\u5b66\u7684\u7814\u7a76\u304c\u3055\u308c\u3066\u3044\u308b\u3002\u30c6\u30ed\u30e1\u30a2\u914d\u5217\u304cG4\u69cb\u9020\u3092\u53d6\u308a\u3046\u308b\u3053\u3068\u304b\u3089\u3001G4\u69cb\u9020\u306b\u7d50\u5408\u3059\u308b\u591a\u304f\u306e\u5316\u5408\u7269\u304c\u540c\u5b9a\u3001\u3042\u308b\u3044\u306f\u5408\u6210\u3055\u308c\u3066\u3044\u308b\u3002\u6771\u4eac\u8fb2\u5de5\u5927\u306e\u9577\u6fa4\u6559\u6388\u306f\u3001G4\u30ea\u30ac\u30f3\u30c9\u306e\u5408\u6210\u3001\u89e3\u6790\u3067\u4e16\u754c\u3092\u30ea\u30fc\u30c9\u3057\u3066\u3044\u308b\u304c\u3001\u6211\u3005\u306f\u5f7c\u3089\u3068\u306e\u5171\u540c\u7814\u7a76\u306b\u3088\u308a\u3001Rif1\u7d50\u5408\u90e8\u4f4d\u304cG4\u69cb\u9020\u3092\u53d6\u308a\u3046\u308b\u4e8b\u3092\u8a3c\u660e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3002\u6700\u8fd1\u3001\u3053\u308c\u3089\u306eG4\u30ea\u30ac\u30f3\u30c9\u3042\u308b\u3044\u306fG4\u69cb\u9020\u7279\u7570\u7684\u306a\u6297\u4f53\u3092\u5229\u7528\u3057\u3066\u3001\u7d30\u80de\u5185\u306b\u304a\u3051\u308bG4\u69cb\u9020\u306e\u5b58\u5728\u304c\u793a\u3055\u308c\u3066\u3044\u308b(Nature Chemistry, 2013\u4ed6)\u3002\u751f\u7269\u5b66\u7684\u6a5f\u80fd\u306b\u3064\u3044\u3066\u306f\u3001\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u306e\u9032\u884c\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u3001\u307e\u305f\u305d\u306e\u963b\u5bb3\u3092\u4e00\u7fa4\u306e\u30d8\u30ea\u30ab\u30fc\u30bc\u304cG4\u69cb\u9020\u3092\u89e3\u96e2\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u89e3\u9664\u3059\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b(Zakian\u3089)\u3002\u307e\u305f\u907a\u4f1d\u5b50\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u4e0a\u306b\u5b58\u5728\u3057\u8ee2\u5199\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u3082\u77e5\u3089\u308c\u3066\u3044\u308b\u3002G4\u69cb\u9020\u3092\u53d6\u308a\u3046\u308b\u914d\u5217\u306f\u5927\u8178\u83cc\u30b2\u30ce\u30e0\u4e0a\u306b6000\u30ab\u6240\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u306b\u306f40\u4e07\u30ab\u6240\u8fd1\u304f\u3082\u3042\u308b\u3068\u63a8\u5b9a\u3055\u308c\u3066\u3044\u308b\u304c\u3001\u305d\u306e\u30b2\u30ce\u30e0\u6a5f\u80fd\u306b\u304a\u3051\u308b\u6b63\u306e\u5f79\u5272\u306f\u3053\u308c\u307e\u3067\u4e0d\u660e\u3067\u3042\u3063\u305f\u3002\u6700\u8fd1\u3001\u30d2\u30c8\u30b2\u30ce\u30e0\u306e\u8907\u88fd\u8d77\u70b9\u8fd1\u508d\u306bG4 \u69cb\u9020\u3092\u3068\u308a\u3046\u308b\u914d\u5217\u304c\u6fc3\u7e2e\u3055\u308c\u3066\u3044\u308b\u3068\u3044\u3046\u5831\u544a\u3055\u308c\u305f\u304c\u3001G4\u69cb\u9020\u306e\u8907\u88fd\u958b\u59cb\u306b\u304a\u3051\u308b\u5f79\u5272\u306f\u307e\u3060\u4e0d\u660e\u3067\u3042\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u7d50\u679c\u306e\u8a73\u7d30\uff1a<\/strong><\/p>\n<p>\u672c\u7814\u7a76\u3067\u306f\u3001\u307e\u305a\u5206\u88c2\u9175\u6bcd\u30b2\u30ce\u30e0\u4e0a\u306b\u304a\u3051\u308bRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u90e8\u4f4d\u3092ChIP-Seq\u6cd5<sup>*8<\/sup>\u306b\u3088\u308a\u6c7a\u5b9a\u3057\u305f(\u56f32)\u3002\u305d\u306e\u7d50\u679c\u300112.5Mb\u306e\u30b2\u30ce\u30e0\u4e0a\u306b35\u500b\u306e\u5f37\u3044\u7d50\u5408\u90e8\u4f4d\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306f\u3044\u305a\u308c\u3082\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3057\u3001\u60c5\u5831\u89e3\u6790\u304b\u3089\u3001S\u671f\u5f8c\u671f\u306b\u958b\u59cb\u3059\u308b\u8907\u88fd\u8d77\u70b9\u306e\u8fd1\u508d\u306b\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u7d50\u5408\u90e8\u4f4d\u306e\u914d\u5217\u89e3\u6790\u304b\u3089\u3001G\u306e\u9023\u7d9a\u914d\u5217\u3092\u542b\u3080\u4fdd\u5b58\u3055\u308c\u305f\u914d\u5217\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u4fdd\u5b58\u914d\u5217\u306e\u307b\u304b\u306b\u3082Rif1\u7d50\u5408\u90e8\u4f4d\u306fG\u306e\u9023\u7d9a\u914d\u5217\u3092\u8907\u6570\u500b\u542b\u3080\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u30b2\u30ce\u30e0\u4e0a\u306eRif1\u7d50\u5408\u914d\u5217\u4e0a\u306e\u4fdd\u5b58\u914d\u5217\u306b\u5909\u7570\u3092\u5c0e\u5165\u3057\u305f\u7d50\u679c\u3001Rif1\u306e\u30af\u30ed\u30de\u30c1\u30f3\u7d50\u5408\u304c\u5c40\u6240\u7684\u306b\u6d88\u5931\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u305d\u306e\u8fd1\u508d\u306e\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u304c\u8131\u5236\u5fa1\u3055\u308c\u3001\u8907\u88fd\u8d77\u70b9\u304cS\u671f\u521d\u671f\u306b\u6d3b\u6027\u5316\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u3002\u3053\u308c\u306f\u3001\u540c\u5b9a\u3057\u305f\u4fdd\u5b58\u914d\u5217\u304cRif1\u306e\u30af\u30ed\u30de\u30c1\u30f3\u7d50\u5408\u3068Rif1\u306e\u6a5f\u80fd\u767a\u73fe\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3059\u3002\u307e\u305f\u3001Rif1\u306f\u7d50\u5408\u90e8\u4f4d\u306e\u8fd1\u508d100kb\u306e\u9818\u57df\u306eDNA\u8907\u88fd\u306e\u958b\u59cb\u306b\u6291\u5236\u7684\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/08987757e6b49a230512da72e792ef9e.tif.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-1803\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/08987757e6b49a230512da72e792ef9e.tif-620x469.jpg\" alt=\"08987757e6b49a230512da72e792ef9e.tif\" width=\"620\" height=\"469\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/08987757e6b49a230512da72e792ef9e.tif-620x469.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/08987757e6b49a230512da72e792ef9e.tif-300x227.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/08987757e6b49a230512da72e792ef9e.tif.jpg 641w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p><strong>\u56f32\u3000\u7814\u7a76\u306e\u6d41\u308c<\/strong><\/p>\n<p>\u6b21\u306b\u3001Rif1\u304c\u3069\u306e\u3088\u3046\u306e\u4fdd\u5b58\u914d\u5217\u306b\u4f9d\u5b58\u3057\u3066Rif1\u7d50\u5408\u9818\u57df\u306b\u7d50\u5408\u3059\u308b\u304b\u3092\u751f\u5316\u5b66\u7684\u306b\u89e3\u6790\u3057\u305f\u3002\u7cbe\u88fd\u3057\u305fRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u4e8c\u672c\u9396DNA\u306b\u7d50\u5408\u3059\u308b\u304c\u3001Rif1\u4fdd\u5b58\u914d\u5217\u3078\u306e\u4f9d\u5b58\u6027\u306f\u89b3\u5bdf\u3055\u308c\u306a\u304b\u3063\u305f\u3002G\u306e\u9023\u7d9a\u914d\u5217\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304b\u3089\u3001G4\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u304b\u3069\u3046\u304b\u3092\u8abf\u3079\u305f\u3002\u305d\u306e\u7d50\u679c\u3001Rif1\u7d50\u5408\u914d\u5217\u3092\u71b1\u5909\u6027\u3057\u3066\u3001\u518d\u30a2\u30cb\u30fc\u30eb\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u4fdd\u5b58\u914d\u5217\u306b\u4f9d\u5b58\u3057\u3066G4\u69d8\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u7cbe\u88fd\u3057\u305fRif1\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001G4\u69cb\u9020\u3092\u5f62\u6210\u3057\u305f\u6642\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3092\u8a3c\u660e\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u308c\u3089\u306e\u767a\u898b\u306f\u3001\u7d30\u80de\u5185\u3067G4\u69cb\u9020\u304c\u5b9f\u969b\u306b\u5f62\u6210\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u308c\u3092\u7279\u7570\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d50\u5408\u3057\u3001\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u907a\u4f1d\u5b66\u7684\u306b\u8a3c\u660e\u3059\u308b(\u56f33)\u3002G4\u69cb\u9020\u306e\u67d3\u8272\u4f53\u5236\u5fa1\u306b\u304a\u3051\u308b\u666e\u904d\u7684\u306a\u5f79\u5272\u306f\u3053\u308c\u307e\u3067\u5168\u304f\u4e0d\u660e\u3060\u3063\u305f\u304c\u3001\u4eca\u56de\u306e\u767a\u898b\u306f\u3001G4\u69cb\u9020\u304c\u67d3\u8272\u4f53\u4e0a\u306e\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3057\u3001\u305d\u308c\u3092\u7279\u7570\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u8a8d\u8b58\u3001\u7d50\u5408\u3057\u3001\u67d3\u8272\u4f53\u306e\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u53ef\u80fd\u6027\u3092\u793a\u3057(\u56f34)\u3001\u3053\u308c\u307e\u3067\u672a\u77e5\u3067\u3042\u3063\u305fG4\u69cb\u9020\u306e\u666e\u904d\u7684\u306a\u751f\u7269\u5b66\u7684\u610f\u7fa9\u306e\u4e00\u7aef\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3082\u306e\u3067\u3042\u308b\u3002<\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/2bc617e017b48bf56f26dcca1601f34a.tif.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-1800\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/2bc617e017b48bf56f26dcca1601f34a.tif-620x461.jpg\" alt=\"2bc617e017b48bf56f26dcca1601f34a.tif\" width=\"620\" height=\"461\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/2bc617e017b48bf56f26dcca1601f34a.tif-620x461.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/2bc617e017b48bf56f26dcca1601f34a.tif-300x223.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/2bc617e017b48bf56f26dcca1601f34a.tif.jpg 629w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p><strong>\u56f3\uff13\u3000Rif1\u306f\u907a\u4f1d\u5b50\u9593\u9818\u57df\u306b\u5b58\u5728\u3059\u308bG4\u69cb\u9020\u306b\u7d50\u5408\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u3092\u675f\u306d\u308b\u3068\u3068\u3082\u306b\u6838\u819c\u8fd1\u508d\u306b\u8907\u88fd\u6291\u5236\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u3002<\/strong><\/p>\n<p><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/14b2b0c77d49aca44c4c9ddff03d91a0.tif.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-1802\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/14b2b0c77d49aca44c4c9ddff03d91a0.tif-620x396.jpg\" alt=\"14b2b0c77d49aca44c4c9ddff03d91a0.tif\" width=\"620\" height=\"396\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/14b2b0c77d49aca44c4c9ddff03d91a0.tif-620x396.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/14b2b0c77d49aca44c4c9ddff03d91a0.tif-300x192.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2015\/09\/14b2b0c77d49aca44c4c9ddff03d91a0.tif.jpg 681w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p><strong>\u56f3\uff14\u3000\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u69cb\u9020\u306e\u751f\u7269\u5b66\u7684\u610f\u7fa9\uff1a\u4eca\u56de\u3001\u67d3\u8272\u4f53\u306e\u9ad8\u6b21\u69cb\u9020\u306e\u5f62\u6210\u5236\u5fa1\u3000\u3068\u3044\u3046\u666e\u904d\u7684\u306a\u6a5f\u80fd\u306e\u4e00\u7aef\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002<\/strong><\/p>\n<p style=\"padding-left: 30px;\">\n<h2><span id=\"Graphic-representations-of-recent-results\">Graphic representations of recent results<\/span><\/h2>\n<p>\u751f\u7269\u306e\u751f\u5b58\u3068\u907a\u4f1d\u306b\u304a\u3044\u3066\u3001\u907a\u4f1d\u60c5\u5831\u306e\u6b63\u78ba\u306a\u500d\u52a0(DNA\u8907\u2028\u88fd)\u3068\u5a18\u7d30\u80de\u3078\u306e\u4f1d\u9054\u306f\u6700\u3082\u57fa\u672c\u7684\u306a\u751f\u547d\u73fe\u8c61\u306e\u4e00\u3064\u3067\u3042\u2028\u308b\u3002\u3053\u308c\u304c\u4fdd\u8a3c\u3055\u308c\u306a\u3044\u3068\u3001\u751f\u547d\u306f\u5b58\u7d9a\u3067\u304d\u306a\u3044\u3053\u3068\u306f\u8a00\u3046\u307e\u2028\u3067\u3082\u306a\u3044\u304c\u3001\u305d\u306e\u904e\u7a0b\u306b\u7570\u5e38\u304c\u751f\u3058\u308b\u3068\u3001\u907a\u4f1d\u60c5\u5831\u306e\u8aa4\u308a\u306e\u84c4\u2028\u7a4d\u3001\u30b2\u30ce\u30e0\u306e\u4e0d\u5b89\u5b9a\u5316\u304c\u3082\u305f\u3089\u3055\u308c\u3001\u7a2e\u3005\u306e\u75be\u60a3\u306e\u539f\u56e0\u3068\u306a\u308b\u3002<\/p>\n<p>\u79c1\u9054\u306f\u3001\u3053\u306eDNA\u8907\u88fd\u306e\u5206\u5b50\u6a5f\u69cb\u306b\u3064\u3044\u3066\u4e3b\u306b3\u3064\u306e\u5074\u9762\u304b\u3089\u30a2\u30d7\u30ed\u30fc\u30c1\u3057\u3066\u3044\u308b\u3002<\/p>\n<p>(1)\u30b2\u30ce\u30e0\u306e\u5b89\u5b9a\u6027\u7dad\u6301\u6a5f\u69cb\u3068\u305d\u306e\u7834\u7dbb\uff1a\u5897\u6b96\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3001\u7d30\u80de\u5468\u671f\u306f\u53b3\u5bc6\u306b\u5236\u5fa1\u3055\u308c\u308b\u3002\u305d\u306e\u7834\u7dbb\u306f\u305f\u3060\u3061\u306b\u3001\u7d30\u80de\u306e\u7570\u5e38\u5897\u6b96\u3001\u7a2e\u3005\u306e\u67d3\u8272\u4f53\u7570\u5e38\u7b49\u3092\u5f15\u304d\u8d77\u3053\u3057\u3001\u304c\u3093\u306a\u3069\u306e\u75be\u60a3\u3001\u6216\u306f\u8001\u5316\u306e\u539f\u56e0\u3068\u306a\u308b\u3002\u79c1\u9054\u306f\u3001\u5927\u8178\u83cc\u30fb\u9175\u6bcd\u30fb\u52d5\u7269\u7d30\u80de\uff08\u6b63\u5e38\u7d30\u80de\u3001\u304c\u3093\u7d30\u80de\u3001\u80da\u6027\u5e79\u7d30\u80de\uff09\u30fb\u500b\u4f53\u3092\u7528\u3044\u3001\u67d3\u8272\u4f53DNA\u8907\u88fd\u5236\u5fa1\u6a5f\u69cb\u53ca\u3073\u67d3\u8272\u4f53\u306e\u5b89\u5b9a\u6027\u7dad\u6301\u6a5f\u69cb\u3092\u89e3\u6790\u3057\u3066\u3044\u308b\u3002\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u5206\u5b50\u6a5f\u69cb\u306b\u57fa\u3065\u304d\u3001\u3053\u308c\u3089\u306e\u904e\u7a0b\u304c\u767a\u751f\u3084\u5206\u5316\u306e\u904e\u7a0b\u3068\u3069\u306e\u3088\u3046\u306b\u95a2\u9023\u3059\u308b\u304b\u3001\u53c8\u305d\u306e\u7570\u5e38\u304c\u3069\u306e\u3088\u3046\u306b\u7a2e\u3005\u306e\u75be\u60a3\u3092\u5f15\u304d\u8d77\u3053\u3059\u304b\u3001\u66f4\u306b\u3001\u75be\u60a3\u306e\u6cbb\u7642\u306e\u65b0\u898f\u5206\u5b50\u6a19\u7684\u306e\u767a\u898b\u3001\u5236\u304c\u3093\u6226\u7565\u30fb\u5275\u85ac\u306e\u958b\u767a\u3092\u76ee\u6307\u3059\u3002<\/p>\n<p>(2)\u00a0\u7d30\u80de\u306e\u5897\u6b96\u3001\u904b\u547d\u3092\u6c7a\u5b9a\u3059\u308b\u67d3\u8272\u4f53\u30c0\u30a4\u30ca\u30df\u30af\u30b9\u306e\u5236\u5fa1\u6a5f\u69cb(\u6838\u5185\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u9020\u306e\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0)\uff1a\u9577\u5927\u306a\u67d3\u8272\u4f53\u306e\u8907\u88fd\u306f\u3001\u53b3\u5bc6\u306a\u6642\u9593\u7684\u7a7a\u9593\u7684\u30d7\u30ed\u30b0\u30e9\u30e0\u306b\u5f93\u3063\u3066\u79e9\u5e8f\u6b63\u3057\u304f\u9032\u884c\u3059\u308b\u3002\u3053\u306e\u904e\u7a0b\u306f\u3001\u6838\u5185\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u9020\u306e\u5236\u5fa1\u3068\u5bc6\u63a5\u306b\u95a2\u9023\u3057\u3001\u8ee2\u5199\u30fb\u7d44\u63db\u3048\u30fb\u4fee\u5fa9\u7b49\u306e\u67d3\u8272\u4f53\u30c0\u30a4\u30ca\u30df\u30af\u30b9\u3068\u3082\u9023\u52d5\u3059\u308b\u3002\u79c1\u9054\u306f\u3001\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u306e\u67d3\u8272\u4f53\u8907\u88fd\u958b\u59cb\u30fb\u9032\u884c\u306e\u6642\u7a7a\u9593\u5236\u5fa1\u6a5f\u69cb\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e\u3057\u3001\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u9020\u3092\u5236\u5fa1\u3059\u308b\u65b0\u898f\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3002<\/p>\n<p>(3)\u67d3\u8272\u4f53\u8907\u88fd\u306e\u666e\u904d\u7684\u30e1\u30ab\u30cb\u30ba\u30e0\u30fb\u305d\u306e\u8d77\u6e90\u3068\u9032\u5316\uff1a\u5927\u8178\u83cc\u3001\u5206\u88c2\u9175\u6bcd\u3001\u52d5\u7269\u7d30\u80de\u66f4\u306b\u5909\u7570\u30de\u30a6\u30b9\u306a\u3069\u591a\u69d8\u306a\u30e2\u30c7\u30eb\u751f\u7269\u3092\u7528\u3044\u3001\u539f\u6838\u7d30\u80de\u304b\u3089\u30d2\u30c8\u307e\u3067\u4fdd\u5b58\u3055\u308c\u305f\u67d3\u8272\u4f53\u8907\u88fd\u306e\u666e\u904d\u7684\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u89e3\u660e\u3092\u76ee\u6307\u3059\u3002\u7279\u306b\u30b2\u30ce\u30e0\u306e\u30ad\u30e3\u30ea\u30a2\u3067\u3042\u308b\u67d3\u8272\u4f53\u306eB\u578b\u3089\u305b\u3093\u69cb\u9020\u4ee5\u5916\u306e\u7279\u6b8aDNA\u69cb\u9020\u30fb\u6838\u5185\u3067\u306e\u67d3\u8272\u4f53\u9ad8\u6b21\u69cb\u7bc9\u30fb\u914d\u7f6e\u304c\u30b2\u30ce\u30e0\u8907\u88fd\u3084\u30b2\u30ce\u30e0\u6a5f\u80fd\u767a\u73fe\u3092\u3069\u306e\u3088\u3046\u306b\u5236\u5fa1\u3059\u308b\u304b\u306b\u6ce8\u76ee\u3057\u3066\u3044\u308b\u3002\u66f4\u306b\u3001\u5f97\u3089\u308c\u305f\u77e5\u898b\u304b\u3089DNA\u8907\u88fd\u306e\u8d77\u6e90\u3068\u9032\u5316\u3092\u8003\u5bdf\u3059\u308b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1535 size-large\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/9e3045539b7c88aebdad52023fef129c-620x465.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"465\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/9e3045539b7c88aebdad52023fef129c-620x465.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/9e3045539b7c88aebdad52023fef129c-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/9e3045539b7c88aebdad52023fef129c.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-1534 size-large alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/86edb6e3df1d2a2624433b6952971b89-620x464.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"464\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/86edb6e3df1d2a2624433b6952971b89-620x464.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/86edb6e3df1d2a2624433b6952971b89-300x224.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/86edb6e3df1d2a2624433b6952971b89.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><a href=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1536 size-large\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b-620x464.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"464\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b-620x464.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b-300x224.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/ee7aab076d6cd2916b433540fb2fe85b.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/a><\/p>\n<p>Cdc7\u30ad\u30ca\u30fc\u30bc\u306f\u8907\u88fd\u958b\u59cb\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u306f\u305f\u3059\u30ea\u30f3\u9178\u5316\u9175\u7d20\u3067\u3042\u308b\u3002\u5206\u88c2\u9175\u6bcd\u3092\u7528\u3044\u3066Cdc7\u30ad\u30ca\u30fc\u30bc\u306e\u8907\u88fd\u3078\u306e\u8981\u6c42\u6027\u3092\u30d0\u30a4\u30d1\u30b9\u3059\u308b\u56e0\u5b50\u3092\u63a2\u7d22\u3057\u305f\u7d50\u679c\u3001\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u5236\u5fa1\u3059\u308bRif1\u3092\u540c\u5b9a\u3057\u305f\u3002Rif1\u306f\u5206\u88c2\u9175\u6bcd\u306e\u307f\u306a\u3089\u305a\u52d5\u7269\u7d30\u80de\u306b\u304a\u3044\u3066\u3082\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u304c\u305d\u306e\u5f8c\u306e\u7814\u7a76\u304b\u3089\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002Rif1\u306f\u3001\u6838\u819c\u8fd1\u508d\u306b\u5c40\u5728\u3057\u3001\u30af\u30ed\u30de\u30c1\u30f3\u30eb\u30fc\u30d7\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001S\u671f\u306e\u4e2d\u671f\u30fb\u5f8c\u671f\u307e\u3067\u8907\u88fd\u3055\u308c\u306a\u3044\u8907\u88fd\u30bf\u30a4\u30df\u30f3\u30b0\u30c9\u30e1\u30a4\u30f3\u3092\u5f62\u6210\u3059\u308b\u53ef\u80fd\u6027\u3092\u6307\u6458\u3057\u305f\u3002<\/p>\n<p style=\"text-align: left;\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1532 size-large\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8d3a363f31f6a13e6fb8f8e83fd03073-620x464.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"464\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8d3a363f31f6a13e6fb8f8e83fd03073-620x464.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8d3a363f31f6a13e6fb8f8e83fd03073-300x224.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/8d3a363f31f6a13e6fb8f8e83fd03073.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p style=\"text-align: left;\">\u5927\u8178\u83cc\u306a\u3069\u539f\u6838\u7d30\u80de\u3067\u306f\u3001\u901a\u5e38\u67d3\u8272\u4f53\u4e0a\u306eoriC\u90e8\u4f4d\u304b\u3089DnaA\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u4f9d\u5b58\u3057\u3066\u958b\u59cb\u3059\u308b\u3002\u3053\u306e\u958b\u59cb\u306f\u5927\u5909\u52b9\u7387\u304c\u3088\u304f\u3001\u5869\u57fa\u914d\u5217\u7279\u7570\u6027\u3082\u6975\u3081\u3066\u9ad8\u3044\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-1531 size-large alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cSDR-620x464.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"464\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cSDR-620x464.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cSDR-300x224.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/cSDR.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p style=\"text-align: left;\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-1533 size-large\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/50cfb7cf251ddd4664d2575e9c9a9386-620x464.jpg\" alt=\"\u0095]\u0089\u00bf\u0088\u00cf\u0088\u00f5\u0089\u00ef\u0094\u00ad\u0095\u008e\u0091\u0097\u00bf1-6-2014\u0089\u00fc\u0092\u00e8.pptx\" width=\"620\" height=\"464\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/50cfb7cf251ddd4664d2575e9c9a9386-620x464.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/50cfb7cf251ddd4664d2575e9c9a9386-300x224.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/50cfb7cf251ddd4664d2575e9c9a9386.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p style=\"text-align: left;\">\u6b64\u308c\u306b\u5bfe\u3057\u3066\u3001\u5927\u8178\u83cc\u3067\u306fDnaA-oriC\u975e\u4f9d\u5b58\u7684\u306a\u8907\u88fd\u958b\u59cb\u69d8\u5f0f\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u3053\u306e\u8907\u88fd\u306fRNA-DNA\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u7279\u7570\u7684\u306bRNA\u3092\u5206\u89e3\u3059\u308bRNaseH\u907a\u4f1d\u5b50\u306e\u6b20\u640d\u682a\u3067\u89b3\u5bdf\u3055\u308c\u3001\u8ee2\u5199\u3068RecA\u7d44\u63db\u3048\u9175\u7d20\u304a\u3088\u3073PriA\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u4f9d\u5b58\u3059\u308b\u3002PriA\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u8907\u88fd\u30d5\u30a9\u30fc\u30af\u304c\u505c\u6b62\u3057\u305f\u6642\u306b\u305d\u308c\u3092\u8a8d\u8b58\u3057\u3001\u8907\u88fd\u306e\u518d\u4f1a\u3092\u4fc3\u9032\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002DnaA-oriC\u975e\u4f9d\u5b58\u7684\u306a\u8907\u88fd\u306f\u3001\u8907\u88fd\u7d42\u7d50\u9818\u57df\u3092\u4e2d\u5fc3\u306b\u5927\u8178\u83cc\u67d3\u8272\u4f53\u306e\u591a\u304f\u306e\u90e8\u4f4d\u304b\u3089\u958b\u59cb\u3059\u308b\u53ef\u80fd\u6027\u304c\u6307\u6458\u3055\u308c\u3066\u3044\u308b\u3002<\/p>\n<p style=\"padding-left: 30px;\"><img decoding=\"async\" loading=\"lazy\" class=\" wp-image-1538 size-large alignnone\" src=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/1ef683c2087f75ffb453644803f23584-620x465.jpg\" alt=\"\u0094\u00f1B\u008c^DNA.pptx\" width=\"620\" height=\"465\" srcset=\"https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/1ef683c2087f75ffb453644803f23584-620x465.jpg 620w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/1ef683c2087f75ffb453644803f23584-300x225.jpg 300w, https:\/\/www.igakuken.or.jp\/genome\/wp-content\/uploads\/2014\/03\/1ef683c2087f75ffb453644803f23584.jpg 750w\" sizes=\"(max-width: 620px) 100vw, 620px\" \/><\/p>\n<p>\u30b2\u30ce\u30e0\u3092\u69cb\u6210\u3059\u308bDNA\u306f\u304a\u3082\u306bB\u578b\u3089\u305b\u3093\u69cb\u9020\u304b\u3089\u306a\u308b\u304c\u3001\u30b2\u30ce\u30e0\u4e0a\u306b\u306f\u3044\u308f\u3086\u308b\u975eB\u578bDNA\u304c\u591a\u304f\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u306f\u305d\u306e\u4e2d\u3067\u3082\u4ee3\u8868\u7684\u306a\u3082\u306e\u3067\u3042\u308b\u3002\u30d2\u30c8\u30b2\u30ce\u30e0\u4e0a\u306b\u3082\uff14\uff10\u4e07\u30ab\u6240\u8fd1\u304f\u5b58\u5728\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u30b0\u30a2\u30cb\u30f3\uff14\u91cd\u9396\u306e\u30b2\u30ce\u30e0\u7dad\u6301\u306b\u304a\u3051\u308b\u666e\u904d\u7684\u306a\u610f\u7fa9\u306f\u307e\u3060\u4e0d\u660e\u306a\u70b9\u304c\u591a\u3044\u304c\u3001\u8907\u88fd\u958b\u59cb\u306a\u3069\u3078\u306e\u95a2\u4e0e\u304c\u6307\u6458\u3055\u308c\u3066\u3044\u308b\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"ContentsResearch Summary of the Masai LaboratoryCurrent Research <a href=\"https:\/\/www.igakuken.or.jp\/genome\/?page_id=2804\">&nbsp;&nbsp;[&#8230;\u7d9a\u304d\u3092\u8aad\u3080]<\/a>","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2804"}],"collection":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2804"}],"version-history":[{"count":7,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2804\/revisions"}],"predecessor-version":[{"id":3003,"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=\/wp\/v2\/pages\/2804\/revisions\/3003"}],"wp:attachment":[{"href":"https:\/\/www.igakuken.or.jp\/genome\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2804"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}