研究成果

Tomoyuki Miyashita, Kanako Murakami, Emi Kikuchi, Kyouko Ofusa, Kyohei Mikami, Kentaro Endo, Takaaki Miyaji, Sawako Moriyama, Kotaro Konno, Hinako Muratani, Yoshinori Moriyama, Masahiko Watanabe, Junjiro Horiuchi, Minoru Saitoe* (2023) Glia transmit negative valence information during aversive learning in Drosophila. Science (in press).

Mari Suzuki, Hiroshi Kuromi, Mayumi Shindo, Nozomi Sakata, Naoko Niimi, Koji Fukui, Minoru Saitoe, Kazunori Sango* (2023) A Drosophila model of diabetic neuropathy reveals a role of proteasome activity in the glia. iScience.

Shintaro Naganos*, Kohei Ueno, Junjiro Horiuchi, Minoru Saitoe (2022) Dopamine activity in projection neurons regulates short-lasting olfactory approach memory in Drosophila. Eur J Neurosci.

Minoru Saitoe*, Shintaro Naganos, Tomoyuki Miyashita, Motomi Matsuno, Kohei Ueno (2022). A non-canonical on-demand dopaminergic transmission underlying olfactory aversive learning. Neurosci Res.

Morstein J, Hofler D, Ueno K, Jurss JW, Walvoord RR, Bruemmer KJ, Rezgui SP, Brewer TF, Saitoe M, Michel BW, Chang CJ* (2020). Ligand-Directed Approach to Activity-Based Sensing: Developing Palladacycle Fluorescent Probes That Enable Endogenous Carbon Monoxide Detection. J. Am. Chem. Soc.

Ueno K*, Morstein J, Ofusa K, Naganos S, Suzuki-Sawano E, Minegishi S, Rezgui SP, Kitagishi H, Michel BW, Chang CJ, Horiuchi J, Saitoe M* (2020). Carbon Monoxide, a Retrograde Messenger Generated in Postsynaptic Mushroom Body Neurons, Evokes Noncanonical Dopamine Release. J. Neurosci.

Suzuki Y, Schenk JE, Tan H, Gaudry Q* (2020). A Population of Interneurons Signals Changes in the Basal Concentration of Serotonin and Mediates Gain Control in the Drosophila Antennal Lobe. Curr. Biol.

Suzuki T, Kohyama K, Moriyama K, Ozaki M, Hasegawa S, Ueno T, Saitoe M, Morio T, Hayashi M, Sakuma H* (2020). Extracellular ADP augments microglial inflammasome and NF-κB activation via the P2Y12 receptor. Eur. J. Immunol.

Akiba M, Sugimoto K, Aoki R, Murakami R, Miyashita T, Hashimoto R, Hiranuma A, Yamauchi J, Ueno T, Morimoto T* (2020). Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster. Eur. J. Neurosci.

Matsuno M, Horiuchi J, Ofusa K, Masuda T, Saitoe M* (2019). Inhibiting glutamate activity during consolidation suppresses age-related long-term memory impairment in Drosophila. iScience.

Ueno K, Morstein J, Ofusa K, Naganos S, Suzuki-Sawano E, Minegishi A, Kitagishi H, Chang C, Horiuchi J, Saitoe M* (2018). Carbon monoxide, a retrograde messenger generated in post-synaptic mushroom body neurons evokes local dopamine release. bioRxiv.

Miyashita T, Kikuchi E, Horiuchi J, Saitoe M* (2018) Long-Term Memory Engram Cells Are Established by c-Fos/CREB Transcriptional Cycling. Cell Rep. 25(10):2716-2728.e3

Sato S, Ueno K, Saitoe M, Sakai T* (2018) Synaptic depression induced by postsynaptic cAMP production in the Drosophila mushroom body calyx. J. Physiol. 596(12):2447-2461

Suzuki-Sawano E+, Ueno K+, Naganos S, Sawano Y, Horiuchi J, Saitoe M* (2017)　A Drosophila ex vivo model of olfactory appetitive learning. Sci. Rep. 18;7(1):17725 (+ equal contribution)

Ueno K*, Suzuki E, Naganos S, Ofusa K, Horiuchi J, Saitoe M*. (2017) Coincident postsynaptic activity gates presynaptic dopamine release to induce plasticity in Drosophila mushroom bodies. eLife, 6, e21076

Hirano Y*, Ihara K, Masuda T, Yamamoto T, Iwata I, Takahashi A, Awata H, Nakamura N, Takakura M, Suzuki Y, Horiuchi J, Okuno H, Saitoe M*. (2017) Shifting transcriptional machinery is required for long-term memory maintenance and modification in Drosophila mushroom bodies. Nat Commun. 7, 13471

Naganos S, Ueno K, Horiuchi J, and Saitoe M*. (2016) Learning defects in Drosophila growth restricted chico mutants are caused by attenuated adenylyl cyclase activity. Mol. Brain 9(1):37

Tomita J, Ueno T, Mitsuyoshi M, Kume S, Kume K*. (2015) The NMDA Receptor Promotes Sleep in the Fruit Fly, Drosophila melanogaster. PLoS one 10(5) e0128101

Matsuno M, Horiuchi J, Yuasa Y, Ofusa K, Miyashita T, Masuda T, Saitoe M*. (2015). Long-term memory formation in Drosophila requires training-dependent glial transcription. J Neurosci 35, 5557-5565. Picked up in “This week in the Journal”

Yamazaki D, Horiuchi J, Ueno K, Ueno T, Saeki S, Matsuno M, Naganos S, Miyashita T, Hirano Y, Nishikawa H, Taoka M, Yamauchi Y, Isobe T, Honda Y, Kodama T, Masuda T, Saitoe M*. (2014) Glial dysfunction causes age-related memory impairment in Drosophila. Neuron 84(4):753-63

Ueno T, Kume K*. (2014) Functional characterization of dopamine transporter in vivo using Drosophila melanogaster behavioral assays. Frontiers in Behavioral Neuroscience 8(303)

Hirano Y, Saitoe M*. (2014) Hunger-driven modulation in brain functions. Brain Nerve. 66, 41-48.

Hirano Y, Saitoe M*. (2013). Hunger and memory; CRTC coordinates long-term memory with the physiological state, hunger. Commun Integr Biol. 6, e25152.

Hirano Y, Masuda T, Naganos S, Matsuno M, Ueno K, Miyashita T, Horiuchi J, Saitoe M*. (2013) Fasting launches CRTC to facilitate long-term memory formation in Drosophila. Science 339(6118):443-6
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Kamimura K, Ueno K, Nakagawa J, Hamada R, Saitoe M, Maeda N*. (2013) Perlecan regulates bidirectional Wnt signaling at the Drosophila neuromuscular junction. J Cell Biol. 200(2):219-33

Ueno K*, Naganos S, Hirano Y, Horiuchi J, Saitoe M*.(2013) Long-term enhancement of synaptic transmission between antennal lobe and mushroom body in cultured Drosophila brain. J. Physiol. 591(Pt 1)287-302

Miyashita T, Oda Y, Horiuchi J, Yin JC, Morimoto T, Saitoe M*. (2012) Mg(2+) Block of Drosophila NMDA Receptors Is Required for Long-Term Memory Formation and CREB-Dependent Gene Expression. Neuron 74(5):887-98.
Naganos S, Horiuchi J, Saitoe M*. (2012) Mutations in the Drosophila insulin receptor substrate, CHICO, impair olfactory associative learning. Neurosci Res. 73(1):49-55
Hirano Y, Kuriyama Y, Miyashita T, Horiuchi J, Saitoe M*. (2012) Reactive oxygen species are not involved in the onset of age-related memory impairment in Drosophila. Genes Brain Behav. 11(1):79-86

Yamazaki D, Horiuchi J, Miyashita T, Saitoe M*. (2010) Acute Inhibition of PKA Activity at Old Ages Ameliorates Age-Related Memory Impairment in Drosophila. J Neurosci 30, 15573-15577

Tamura T, Horiuchi D, Chen YC, Sone M, Miyashita T, Saitoe M, Yoshimura N, Chiang AS, Okazawa H*. (2010) Drosophila PQBP1 regulates learning acquisition at projection neurons in aversive olfactory conditioning. J Neurosci 30, 14091-14101

Kuromi H*, Ueno K, Kidokoro Y.(2010) Two types of Ca2+ channel linked to two endocytic pathways coordinately maintain synaptic transmission at the Drosophila synapse.　Eur J Neurosci. 32, 335-46.

Matsuno M, Horiuchi J, Tully T, Saitoe M*. (2009). The Drosophila cell adhesion molecule klingon is required for long-term memory formation and is regulated by Notch. Proc Natl Acad Sci U S A. 106, 310-315

Horiuchi J, Yamazaki D, Naganos S, Aigaki T, Saitoe M*. (2008). Protein kinase A inhibits a consolidated form of memory in Drosophila. Proc Natl Acad Sci U S A. 105, 20976-20981

Ueno K*, Kidokoro Y. (2008). Adenylyl cyclase encoded by AC78C participates in sugar perception in Drosophila melanogaster. Eur J Neurosci. 28, 1956-1966
Qi ML, Tagawa K, Enokido Y, Yoshimura N, Wada YI, Watase K, Ishiura SI, Kanazawa I, Botas J, Saitoe M, Wanker EE, Okazawa H*. (2007). Proteome analysis of soluble nuclear proteins reveals that HMGB1/2 suppress genotoxic stress in polyglutamine diseases. Nat Cell Biol 9, 402-414

Yamazaki D, Horiuchi J, Nakagami Y, Nagano S, Tamura T, Saitoe M*. (2007). The Drosophila DC0 mutation suppresses age-related memory impairment without affecting lifespan. Nat Neurosci 10, 478-484

Hoshino M, Qi ML, Yoshimura N, Miyashita T, Tagawa K, Wada Y, Enokido Y, Marubuchi S, Harjes P, Arai N, Oyanagi K, Blandino G, Sudol M, Rich T, Kanazawa I, Wanker EE, Saitoe M, Okazawa H*. (2006). Transcriptional repression induces a slowly progressive atypical neuronal death associated with changes of YAP isoforms and p73. J Cell Biol 172, 589-604

Saitoe M, Tamura T, Ito N, Horiuchi J. (2005). Drosophila as a novel animal model for studying the genetics of age-related memory impairment. Rev Neurosci 16, 137-149

Xia S+, Miyashita T+, Fu T-G+, Lin W-Y, Wu C-L, Pyzocha L, Lin I-R, Saitoe M, Tully T, Chiang A-S*. (2005). NMDA receptors mediate olfactory learning and memory in Drosophila. Current Biology 15, 603-615 (+ equal contribution)

Tamura T, Chiang AS, Ito N, Liu HP, Horiuchi J, Tully T, Saitoe M. (2003). Aging specifically impairs amnesiac-dependent memory in Drosophila. Neuron 40, 1003-1011

Saitoe M*, Schwarz TL, Umbach JA, Gundersen CB, Kidokoro Y. (2001). Absence of junctional glutamate receptor clusters in Drosophila mutants lacking spontaneous transmitter release. Science 293, 514-517


和雑誌

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平野恭敬、齊藤　実　(2017)高次脳機能に関わる転写因子「基礎分子生物学�U：遺伝子発現制御機構―クロマチン，転写制御，エピジェネティクス―」18章1節東京化学同人

長野慎太郎、齊藤実（2016）ショウジョウバエだからわかる学習記憶のメカニズム 生体の科学 2016 Vol67. No.1. 13-16

齊藤実、堀内純二郎、山崎大介(2015) 加齢による記憶力の低下とグリア細胞　Clinical Neuroscience 月刊臨床神経科学 33(9), 1084-1085

上野太郎、粂和彦（2015）ショウジョウバエを用いた睡眠の基礎研究 日本薬理学雑誌 Vol. 145(3), 134-139

平野恭敬、齊藤　実　(2013)　記憶改善に向けた新たな進展：軽度の空腹状態は長期記憶形成を促進する　細胞工学32, 452-453.

平野恭敬、齊藤 実 (2013) ショウジョウバエにおいて空腹な状態はCRTCを活性化させ長期記憶の形成を促進する. ライフサイエンス 新着論文レビュー

宮下知之、齊藤 実　(2012) NMDA受容体におけるMg2+ブロックはCREBに依存性の長期記憶に関連する遺伝子の発現誘導に必須である. ライフサイエンス 新着論文レビュー

松野元美、齊藤　実（2010）学習・記憶と分子マーカー　Clinical Neuroscience Vol.28 No.12 1375-1379

平野恭敬、齊藤　実（2010）脳の老化：加齢性記憶障害とその分子機構　Medical Bio 「老化と加齢性疾患を科学する」号 24-29

長野慎太郎、齊藤　実（2010）加齢性記憶障害とヒストンのアセチル化　実験医学 28(16) 2628-2629

上野耕平、齊藤　実（2009）記憶学習の精緻な理解に向けてのモデル動物：ハエ　生体の科学 61(1) 17-23

山崎大介、齊藤 実 (2008). 加齢性記憶障害への分子メカニズムへのアプローチ BRAIN and NERVE 60, 717-724

上野耕平 (2008) 味を感じる分子メカニズム　昆虫ミメティックス 363-369

宮下知之、齊藤 実 (2006) ショウジョウバエによる記憶学習の分子遺伝学的研究　「脳機能研究の新展開」実験医学増刊号 24, 2347-2354

齊藤 実 (2005) 個体老化と脳の老化の分子遺伝学　医学のあゆみ 215, 651-654

齊藤 実 (2005) 脳の老化の分子遺伝学　基礎老化研究 29, 15-24

齊藤 実 (2005) 加齢性記憶障害のメカニズム　細胞工学 24, 847-853

齊藤 実、田村拓也、堀内純二郎、伊東直美　(2004) ハエから探る老人痴呆:ショウジョウバエによる加齢性記憶障害と老化の分子メカニズムの解析 日本精神神経薬理学雑誌 24, 231-237

齊藤 実 (2004) ショウジョウバエによる学習記憶関連遺伝子の同定とそのシナプス機能の解析 ブレインサイエンスレヴュー (2004). 109-123