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タイトル: Sequence variation and structural conservation in the D-loop region and flanking genes of mitochondrial DNA from Japanese pond frogs
タイトル（英）: Sequence variation and structural conservation in the D-loop region and flanking genes of mitochondrial DNA from Japanese pond frogs
参照URL: https://researchmap.jp/Yonejii20030920/published_papers/37251820
著者: Sumida Masayuki,Kaneda Hideki,Kato Yoji,KANAMORI Yasushi,YONEKAWA Hiromichi,NISHIOKA Midori
著者（英）: SUMIDA Masayuki,KANEDA Hideki,KATO Yoji,KANAMORI Yasushi,YONEKAWA Hiromichi,NISHIOKA Midori
担当区分
概要: The nucleotide sequences of the D-loop region and its flanking genes of the mitochondrial DNA (mtDNA) from Japanese pond frogs were determined by the methods of PCR, cloning, and sequencing. The frogs belonged to two species, one subspecies, and one local race. The gene arrangements adjacent to the D-loop region were analyzed. The frogs shared a unique mitochondrial gene order that was found in Rana catesbeiana; i.e., cyt b - D-loop region -tRNALeu(CUN) - tRNAThr - tRNAPro -tRNAPhe - 12S rRNA. The arrangements of the three tRNA genes of these frogs were different from those of X. laevis, a species which has the same overall structure as in mammals. Highly repetitive sequences with repeat units (16-bp or 17-bp sequence specific for each taxon) were found in the D-loop region. The length of repetitive sequences varied from 0.6 kbp to 1.2 kbp, and caused the extensive size variation in mtDNA. Several short sequence elements such as putative TAS, Oh, CSB-1, and CSB-2 were found in the D-loop region of these frogs. The sequences of these short regulatory elements were conserved in R. catesbeiana, X. laevis, and also in human. The comparison of sequence divergences of the D-loop region and its adjacent genes among various taxa revealed that the rates of nucleotide substitutions depend on genes. The nucleotide sequences of the 3'-side segment of the D-loop region were the most variable among taxa, whereas those of the tRNA and 12S rRNA genes were the most conservative.
概要（英）: The nucleotide sequences of the D-loop region and its flanking genes of the mitochondrial DNA (mtDNA) from Japanese pond frogs were determined by the methods of PCR, cloning, and sequencing. The frogs belonged to two species, one subspecies, and one local race. The gene arrangements adjacent to the D-loop region were analyzed. The frogs shared a unique mitochondrial gene order that was found in Rana catesbeiana; i.e., cyt b - D-loop region -tRNALeu(CUN) - tRNAThr - tRNAPro -tRNAPhe - 12S rRNA. The arrangements of the three tRNA genes of these frogs were different from those of X. laevis, a species which has the same overall structure as in mammals. Highly repetitive sequences with repeat units (16-bp or 17-bp sequence specific for each taxon) were found in the D-loop region. The length of repetitive sequences varied from 0.6 kbp to 1.2 kbp, and caused the extensive size variation in mtDNA. Several short sequence elements such as putative TAS, Oh, CSB-1, and CSB-2 were found in the D-loop region of these frogs. The sequences of these short regulatory elements were conserved in R. catesbeiana, X. laevis, and also in human. The comparison of sequence divergences of the D-loop region and its adjacent genes among various taxa revealed that the rates of nucleotide substitutions depend on genes. The nucleotide sequences of the 3'-side segment of the D-loop region were the most variable among taxa, whereas those of the tRNA and 12S rRNA genes were the most conservative.
出版者・発行元: The Genetics Society of Japan
出版者・発行元（英）: The Genetics Society of Japan
誌名: Genes & genetic systems
誌名（英）: The Japanese Journal of Genetics
巻: 75
号: 2
開始ページ: 79
終了ページ: 92
出版年月: 2000年4月
査読の有無
招待の有無
掲載種別
ISSN: 1341-7568
DOI URL: https://doi.org/10.1266/ggs.75.79
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