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論文
- タイトル
- タイトル（英）
- Silver diagnosis in neuropathology: principles, practice and revised interpretation
- 参照URL
- https://researchmap.jp/brain-pathology/published_papers/1663031
- 著者
- 著者（英）
- Toshiki Uchihara
- 担当区分
- 概要
- 概要（英）
- Silver-staining methods are helpful for histological identification of pathological deposits. In spite of some ambiguities regarding their mechanism and interpretation, they are widely used for histopathological diagnosis. In this review, four major silver-staining methods, modified Bielschowsky, Bodian, Gallyas (GAL) and Campbell-Switzer (CS) methods, are outlined with respect to their principles, basic protocols and interpretations, thereby providing neuropathologists, technicians and neuroscientists with a common basis for comparing findings and identifying the issues that still need to be clarified. Some consider "argyrophilia" to be a homogeneous phenomenon irrespective of the lesion and the method. Thus, they seek to explain the differences among the methods by pointing to their different sensitivities in detecting lesions (quantitative difference). Comparative studies, however, have demonstrated that argyrophilia is heterogeneous and dependent not only on the method but also on the lesion (qualitative difference). Each staining method has its own lesion-dependent specificity and, within this specificity, its own sensitivity. This "method- and lesion-dependent" nature of argyrophilia enables operational sorting of disease-specific lesions based on their silver-staining profiles, which may potentially represent some disease-specific aspects. Furthermore, comparisons between immunohistochemical and biochemical data have revealed an empirical correlation between GAL+/CS-deposits and 4-repeat (4R) tau (corticobasal degeneration, progressive supranuclear palsy and argyrophilic grains) and its complementary reversal between GAL-/CS+deposits and 3-repeat (3R) tau (Pick bodies). Deposits containing both 3R and 4R tau (neurofibrillary tangles of Alzheimer type) are GAL+/CS+. Although no molecular explanations, other than these empiric correlations, are currently available, these distinctive features, especially when combined with immunohistochemistry, are useful because silver-staining methods and immunoreactions are complementary to each other.
- 出版者・発行元
- 出版者・発行元（英）
- SPRINGER
- 誌名
- 誌名（英）
- ACTA NEUROPATHOLOGICA
- 巻
- 113
- 号
- 5
- 開始ページ
- 483
- 終了ページ
- 499
- 出版年月
- 2007年5月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- ISSN
- 0001-6322
- DOI URL
- https://doi.org/10.1007/s00401-007-0200-2
- 共同研究・競争的資金等の研究課題
研究者
内原 俊記
(ウチハラ トシキ)