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論文
- タイトル
- タイトル（英）
- Silver stains distinguish tau-positive structures in corticobasal degeneration/progressive supranuclear palsy and in Alzheimer's disease - Comparison between Gallyas and Campbell-Switzer methods
- 参照URL
- https://researchmap.jp/brain-pathology/published_papers/1663051
- 著者
- 著者（英）
- T Uchihara,K Shibuya,A Nakamura,S Yagishita
- 担当区分
- 概要
- 概要（英）
- Possible differences in silver-staining profiles and their relation to tau-like immunoreactivity were investigated on cortical sections from corticobasal degeneration (CBD), progressive supranuclear palsy (PSP), Down's syndrome (DS) and Alzheimer's disease (AD). Pairs of mirror sections were double-fluorolabeled with an anti-PHF tau (AT8) antibody and thiazin red (TR), a fluorochrome that labels fibrillary structures such as neurofibrillary tangles (NFTs). Subsequently, one of the pair was stained with Gallyas method (GAL), and the other with Campbell-Switzer method (CS). Identification of the same structure on the corresponding microscopic fields enabled a comparison of four different profiles of each structure: AT8 immunoreactivity, and affinity to TR, GAL and CS. NFTs of DS/AD, containing three- and four-repeat tau, were positive for TR, GAL and CS. AT8-immunoreactive structures of CBD/PSP, containing mainly four-repeat tau, were positive for GAL, but negative for CS and TR. This discrepancy is explainable if the argyrophilia with GAL is related to deposits containing four-repeat tau, while that with CS is linked to those containing three-repeat tau. The lack of CS labeling may also be related to poor TR staining, possibly representing scarcity of fibrillary structure in CBD/PSP. The absence of CS staining is characteristic of tau-positive structures of CBD/PSP, which is readily distinguishable from NFTs of DS/AD, hence is of potential pathological and diagnostic relevance.
- 出版者・発行元
- 出版者・発行元（英）
- SPRINGER
- 誌名
- 誌名（英）
- ACTA NEUROPATHOLOGICA
- 巻
- 109
- 号
- 3
- 開始ページ
- 299
- 終了ページ
- 305
- 出版年月
- 2005年4月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0001-6322
- DOI URL
- https://doi.org/10.1007/s00401-004-0947-7
- 共同研究・競争的資金等の研究課題
研究者
内原 俊記
(ウチハラ トシキ)