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タイトル: Ca2+イメージセンサーを用いた海馬の細胞外Ca2+の可視化
タイトル（英）: Applying Ca2+ Image Sensor to visualize extracellular Ca2+ dynamics in hippocampus.
参照URL: https://researchmap.jp/read0091842/published_papers/37812663
著者: パラジュリビージェイ,土井英生,繁冨英治,篠崎陽一,野田俊彦,高橋一浩,澤田和明,小泉修一
著者（英）: Bijay Parajuli,Doi Hideo,Shigetomi Eiji,Shinozaki Youichi,Noda Toshihiko,Takahashi Kazuhiro,Sawada Kazuaki,Koizumi Schuichi
担当区分
概要: Unlike intracellular Ca2+, extracellular Ca2+ ([Ca2+]o) has received only limited attention. [Ca2+]o has profound effects on neuronal membrane potential and synaptic transmission. Physiological conditions like sensory stimulation, sleep-wake transitions and pathological conditions like seizures, ischemia are reported to decrease [Ca2+]o. However, these data are obtained from a single point analysis using a Ca2+ electrode and lack spatiotemporal information of [Ca2+]o changes. To understand processes shaping local and global [Ca2+]o change involved in regulating physiological and pathological neuronal activity, we need an imaging technique that provides higher spatiotemporal resolution. Thus, we developed a label-free [Ca2+]o image sensor (CIS) with a time resolution of a few tens of milliseconds at 23.55 mm pitch of 128 x 128 pixel. The CIS is highly selective to Ca2+ but not to other cations within wide dynamic range (from 100 mM to 100 mM). We used CIS for the imaging of [Ca2+]o in acute hippocampal slices. Stimulation with neurotransmitter glutamate (Glu), decreased [Ca2+]o to around 800 mM within 10 s, which gradually returned to the baseline level (2 mM) with slow kinetics (5 min). The Glu-evoked [Ca2+]o decrease was higher in CA1 and DG compared to CA3, and was inhibited by a NMDA receptor antagonist D-APV but not AMPA receptor antagonist CNQX. NMDA mimicked the Glu-evoked [Ca2+]o decrease and, again was inhibited by D-APV. These findings suggest that Glu acts on neuronal NDMA receptors to reduce [Ca2+]o with different spatiotemporal patterns, reflecting differences in NMDA receptors distribution. Our study demonstrates that a CIS can detect [Ca2+]o changes associated with biological events and have broad applicability in future biological studies.
概要（英）: Unlike intracellular Ca2+, extracellular Ca2+ ([Ca2+]o) has received only limited attention. [Ca2+]o has profound effects on neuronal membrane potential and synaptic transmission. Physiological conditions like sensory stimulation, sleep-wake transitions and pathological conditions like seizures, ischemia are reported to decrease [Ca2+]o. However, these data are obtained from a single point analysis using a Ca2+ electrode and lack spatiotemporal information of [Ca2+]o changes. To understand processes shaping local and global [Ca2+]o change involved in regulating physiological and pathological neuronal activity, we need an imaging technique that provides higher spatiotemporal resolution. Thus, we developed a label-free [Ca2+]o image sensor (CIS) with a time resolution of a few tens of milliseconds at 23.55 mm pitch of 128 x 128 pixel. The CIS is highly selective to Ca2+ but not to other cations within wide dynamic range (from 100 mM to 100 mM). We used CIS for the imaging of [Ca2+]o in acute hippocampal slices. Stimulation with neurotransmitter glutamate (Glu), decreased [Ca2+]o to around 800 mM within 10 s, which gradually returned to the baseline level (2 mM) with slow kinetics (5 min). The Glu-evoked [Ca2+]o decrease was higher in CA1 and DG compared to CA3, and was inhibited by a NMDA receptor antagonist D-APV but not AMPA receptor antagonist CNQX. NMDA mimicked the Glu-evoked [Ca2+]o decrease and, again was inhibited by D-APV. These findings suggest that Glu acts on neuronal NDMA receptors to reduce [Ca2+]o with different spatiotemporal patterns, reflecting differences in NMDA receptors distribution. Our study demonstrates that a CIS can detect [Ca2+]o changes associated with biological events and have broad applicability in future biological studies.
出版者・発行元: 公益社団法人日本薬理学会
出版者・発行元（英）: Japanese Pharmacological Society
誌名: 日本薬理学会年会要旨集
誌名（英）: Proceedings for Annual Meeting of The Japanese Pharmacological Society
巻: 94
号
開始ページ: 1-P1-10
終了ページ
出版年月: 2021年
査読の有無
招待の有無
掲載種別
ISSN
DOI URL: https://doi.org/10.1254/jpssuppl.94.0_1-p1-10
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研究者

篠﨑陽一 (シノザキヨウイチ)