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論文
- タイトル
- タイトル（英）
- Label-Free Real-Time Imaging of Extracellular Lactate from a Hippocampal Slice Based on Charge-Transfer-Type Potentiometric Redox Sensor Arrays
- 参照URL
- https://researchmap.jp/read0091842/published_papers/27771128
- 著者
- 著者（英）
- Hideo Doi,Tomoko Horio,Eiji Shigetomi,Youichi Shinozaki,You Na Lee,Tatsuya Yoshimi,Tatsuya Iwata,Toshihiko Noda,Kazuhiro Takahashi,Toshiaki Hattori,Schuichi Koizumi,Kazuaki Sawada
- 担当区分
- 概要
- 概要（英）
- © 2019 IEEE. Medically important real-time imaging of extracellular lactate and its spatiotemporal analysis has not yet been achieved. If it could be successfully realized, it would help clarify the function of physiological neurotransmission and drug discovery based on glial cell neuron lactate transport. To this end, we fabricated a 40 μm pitch, 128 × 128 pixel redox-type label-free lactate image sensor based on a potentiometric hydrogen peroxide (H2O2) sensor array and successfully obtained a diffusion image of lactate. Further, the fabricated sensor was applied to extracellular imaging of tissue from a mouse hippocampus. A 300-μm-thick slice of the hippocampus was placed on a lactate image sensor, and when stimulated by glutamate, the output image of the sensor changed dynamically. The output voltage change (ΔVOut) of the sensor was approximately 140 mV. Conversely, verification by a comparative experiment using an enzyme-free sensor confirmed a significant difference in the value of ΔVOut. It can thus be concluded that the observation of lactate in solution was demonstrated successfully by label-free real-time imaging of lactate from mouse hippocampus tissue with glutamate stimulation on a 40 μm-pitch sensor array.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
- 巻
- 号
- 開始ページ
- 617
- 終了ページ
- 620
- 出版年月
- 2019年6月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（国際会議プロシーディングス）
- ISSN
- DOI URL
- https://doi.org/10.1109/TRANSDUCERS.2019.8808447
- 共同研究・競争的資金等の研究課題
研究者
篠﨑 陽一
(シノザキ ヨウイチ)