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MISC
- タイトル
- タイトル（英）
- MOLECULAR-CLONING AND CHARACTERIZATION OF A 3RD TYPE OF N-GLYCAN ALPHA-2,8-SIALYLTRANSFERASE FROM MOUSE LUNG
- 参照URL
- https://researchmap.jp/read0000903/misc/42297799
- 著者
- 著者（英）
- Y YOSHIDA,N KOJIMA,S TSUJI
- 担当区分
- 概要
- 概要（英）
- A cDNA encoding a new alpha 2,8-sialyltransferase (ST8Sia IV), which exhibits activity toward the alpha 2,3-linked sialic acids of N-linked oligosaccharides, was cloned from a mouse lung cDNA library by means of the PCR-based approach, The predicted amino acid sequence of ST8Sia IV showed 15.2, 56.0, and 26.2% identity with those of so far cloned mouse alpha 2,8-sialyltransferases, i.e., GD3 synthase (ST8Sia I), STX (ST8Sia II), and Sia alpha 2,3Gal beta 1-4GlcNAc alpha 2,8-sialyltransferase (ST8Sia III). ST8Sia IV exhibits high amino acid sequence identity (99.2%) with recently cloned hamster polysialyltransferase-1 gene, which is necessary to polysialic acid expression, but no enzymatic activity of the gene product was reported [Eckhardt, M. et al, (1995) Nature 373, 715-7183]. The ST8Sia IV gene was strongly expressed in lung, heart, and spleen, but only weak expression of the gene was observed in brain, without remarkable developmental regulation, The activity of mouse ST8Sia IV was specific toward sialylated glycoproteins, The linkage-specific sialidase treatment of glycoproteins as well as N-linked oligosaccharides from the glycoproteins revealed that STSSia IV exhibits an alpha 2,8-sialyltransferase activity toward alpha 2,3-linked sialic acids of N-linked oligosaccharides. In addition, STSSia IV can synthesize polysialic acid chain in vitro without any initiator sialyltransferase.
- 出版者・発行元
- 出版者・発行元（英）
- JAPAN BIOCHEMICAL SOC
- 誌名
- 誌名（英）
- JOURNAL OF BIOCHEMISTRY
- 巻
- 118
- 号
- 3
- 開始ページ
- 658
- 終了ページ
- 664
- 出版年月
- 1995年9月
- 査読の有無
- 招待の有無
- 掲載種別
- ISSN
- 0021-924X
- DOI URL
- 共同研究・競争的資金等の研究課題
研究者
吉田 雪子
(ヨシダ ユキコ)