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論文
- タイトル
- タイトル（英）
- Co-Administration of Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein Increases Masseter Muscle Mass and Fiber Size
- 参照URL
- https://researchmap.jp/read0160429/published_papers/44327867
- 著者
- 著者（英）
- Od Bayarsaikhan,Nobuhiko Kawai,Hiroyo Mori,Nao Kinouchi,Takeshi Nikawa,Eiji Tanaka
- 担当区分
- 概要
- 概要（英）
- Myostatin, a member of the TGF-beta superfamily, is a negative regulator of skeletal muscle cell growth and differentiation, and binds with high affinity to the activin type IIB receptor (ActRIIB). The soluble ligand-binding domain of ActRIIB fused to the Fc domain of IgG (ActRIIB-Fc) potently binds and inhibits TGF-beta family members in muscle, leading to rapid and marked muscle growth. The present study was designed to assess the effectiveness of the co-delivery of myostatin-targeting siRNA (Mstn-siRNA) and ActRIIB-Fc into skeletal muscle as a potential treatment of atrophic myopathies. Eleven-week-old, male C57BL/6 mice were injected with atelocollagen (ATCOL)-mediated Mstn-siRNA with/without ActRIIB-Fc locally into the masseter muscle twice a week. Inhibition of myostatin function by the combination of Mstn-siRNA and ActRIIB-Fc increased muscle weight and myofibril size in murine masseter muscle. Real-time RT-PCR analysis revealed significant downregulation of myostatin mRNA expression in both the Mstn-siRNA-treated and the combination treatment group. Furthermore, myogenin mRNA expression was upregulated in the combination treatment group, while MuRF-1 and Atrogin-1 mRNA expression was downregulated compared to administration of each compound alone. These findings suggest that double inhibition of myostatin is a potentially useful treatment strategy to increase muscle mass and fiber size and could be a useful treatment of patients with various muscle atrophies, including muscular dystrophy.
- 出版者・発行元
- 出版者・発行元（英）
- CENTER ACADEMIC PUBL JAPAN
- 誌名
- 誌名（英）
- JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
- 巻
- 63
- 号
- 4
- 開始ページ
- 244
- 終了ページ
- 248
- 出版年月
- 2017年8月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0301-4800
- DOI URL
- https://doi.org/10.3177/jnsv.63.244
- 共同研究・競争的資金等の研究課題
研究者
田中 啓二
(タナカ ケイジ)