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論文
- タイトル
- タイトル（英）
- Flavones Inhibit LPS-Induced Atrogin-1/MAFbx Expression in Mouse C2C12 Skeletal Myotubes
- 参照URL
- https://researchmap.jp/read0160429/published_papers/44327870
- 著者
- 著者（英）
- Chieko Shiota,Tomoki Abe,Nobuhiko Kawai,Ayako Ohno,Shigetada Teshima-Kondo,Hiroyo Mori,Junji Terao,Eiji Tanaka,Takeshi Nikawa
- 担当区分
- 概要
- 概要（英）
- Muscle atrophy is a complex process that occurs as a consequence of various stress events. Muscle atrophy-associated genes (atrogenes) such as atrogin-1/IVIAFbx and MuRF-1 are induced early in the atrophy process, and the increase in their expression precedes the loss of muscle weight. Although antioxidative nutrients suppress atrogene expression in skeletal muscle cells, the inhibitory effects of flavonoids on inflammation-induced atrogin-1/MAFbx expression have not been clarified. Here, we investigated the inhibitory effects of flavonoids on lipopolysaccharide (LPS)-induced atrogin-1/MAFbx expression. We examined whether nine flavonoids belonging to six flavonoid categories inhibited atrogin-1/MAFbx expression in mouse C2C12 myotubes. Two major flavones, apigenin and luteolin, displayed potent inhibitory effects on atrogin-1/MAFbx expression. The pretreatment with apigenin and luteolin significantly prevented the decrease in C2C12 myotube diameter caused by LPS stimulation. Importantly, the pretreatment of LPS-stimulated myoblasts with these flavones significantly inhibited LPS-induced JNK phosphorylation in C2C12 myotubes, resulting in the significant suppression of atrogin-1/MAFbx promoter activity These results suggest that apigenin and luteolin, prevent LPS-mediated atrogin-1/MAFbx expression through the inhibition of the JNK signaling pathway in C2C12 myotubes. Thus, these flavones, apigenin and luteolin, may be promising agents to prevent LPS-induced muscle atrophy.
- 出版者・発行元
- 出版者・発行元（英）
- CENTER ACADEMIC PUBL JAPAN
- 誌名
- 誌名（英）
- JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
- 巻
- 61
- 号
- 2
- 開始ページ
- 188
- 終了ページ
- 194
- 出版年月
- 2015年4月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0301-4800
- DOI URL
- https://doi.org/10.3177/jnsv.61.188
- 共同研究・競争的資金等の研究課題
研究者
田中 啓二
(タナカ ケイジ)