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論文
- タイトル
- タイトル(英)
- Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor
- 参照URL
- https://researchmap.jp/tabe/published_papers/1003929
- 著者
- 著者(英)
- Takashi Abe,Tsuyoshi Mikekado,Satoru Haga,Yuya Kisara,Kohei Watanabe,Tadahide Kurokawa,Tohru Suzuki
- 担当区分
- 概要
- 概要(英)
- Leukemia inhibitory factor (LIF) maintains embryonic stem cells in an undifferentiated state. To establish stable cultures of zebrafish embryonic stem cells, recombinant zebrafish LIF (zLIF) is needed because the LIF sequence varies greatly between species. In the current study, we identified the zebrafish (Danio rerio) and pufferfish (Tetraodon nigroviridis) orthologs of lif from genomic databases, and we isolated a cDNA encoding zLIF. Synteny analysis and comparison of sequences identified zebrafish and Tetraodon orthologs of human LIF. The cDNA for zLIF encoded a predicted 215-amino acid protein with a putative 32-amino acid signal peptide, two disulfide bonds, and two N-linked glycosylation sites. We found that transcription of zlif starts at the hatching period during embryogenesis and is present in the brain, visceral organs, bone, and skin. (c) 2007 Elsevier Inc. All rights reserved.
- 出版者・発行元
- 出版者・発行元(英)
- ELSEVIER SCIENCE INC
- 誌名
- 誌名(英)
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
- 巻
- 147
- 号
- 1
- 開始ページ
- 38
- 終了ページ
- 44
- 出版年月
- 2007年5月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文(学術雑誌)
- ISSN
- 1096-4959
- DOI URL
- https://doi.org/10.1016/j.cbpb.2006.12.019
- 共同研究・競争的資金等の研究課題
研究者
阿部 崇志
(アベ タカシ)