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論文
- タイトル
- タイトル（英）
- Liver target delivery of small interfering RNA to the HCV gene by lactosylated cationic liposome.
- 参照URL
- https://researchmap.jp/read0088287/published_papers/46131426
- 著者
- 著者（英）
- Tsunamasa Watanabe,Takuya Umehara,Fumihiko Yasui,Shin-Ichiro Nakagawa,Junichi Yano,Tadaaki Ohgi,Satoru Sonoke,Kenichi Satoh,Kazuaki Inoue,Makoto Yoshiba,Michinori Kohara
- 担当区分
- 概要
- 概要（英）
- BACKGROUND/AIMS: RNA interference has considerable therapeutic potential, particularly for anti-viral therapy. We previously reported that hepatitis C virus (HCV)-directed small interfering RNA (siRNA; siE) efficiently inhibits HCV replication, using HCV replicon cells. To employ the siRNA as a therapeutic strategy, we attempted in vivo silencing of intrahepatic HCV gene expression by siE using a novel cationic liposome. METHODS: The liposomes consisted of conjugated lactose residues, based on the speculation that lactose residues would effectively deliver siRNA to the liver via a liver specific receptor. The lactosylated cationic liposome 5 (CL-LA5) that contained the most lactose residues introduced the most siRNA into a human hepatoma cell line, which then inhibited replication of HCV replicons. RESULTS: In mice, the siRNA/CL-LA5 complexes accumulated primarily in the liver and were widespread throughout the hepatic parenchymal cells. Moreover, siE/CL-LA5 specifically and dose-dependently suppressed intrahepatic HCV expression in transgenic mice without an interferon response. CONCLUSIONS: The present results indicate that the CL-LA5 we developed is a good vehicle to lead siRNA to the liver. Hence, CL-LA5 will be helpful for siRNA therapy targeting liver diseases, especially hepatitis C.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Journal of hepatology
- 巻
- 47
- 号
- 6
- 開始ページ
- 744
- 終了ページ
- 50
- 出版年月
- 2007年12月
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0168-8278
- DOI URL
- 共同研究・競争的資金等の研究課題
研究者
安井 文彦
(ヤスイ フミヒコ)