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論文
- タイトル
- タイトル（英）
- Production of recombinant beta-hexosaminidase A, a potential enzyme for replacement therapy for Tay-Sachs and Sandhoff diseases, in the methylotrophic yeast Ogataea minuta.
- 参照URL
- https://researchmap.jp/youichi01/published_papers/46293623
- 著者
- 著者（英）
- Hiromi Akeboshi,Yasunori Chiba,Yoshiko Kasahara,Minako Takashiba,Yuki Takaoka,Mai Ohsawa,Youichi Tajima,Ikuo Kawashima,Daisuke Tsuji,Kohji Itoh,Hitoshi Sakuraba,Yoshifumi Jigami
- 担当区分
- 概要
- 概要（英）
- Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount of HexA for a treatment based on enzyme replacement therapy (ERT), recombinant HexA was produced in the methylotrophic yeast Ogataea minuta instead of in mammalian cells, which are commonly used to produce recombinant enzymes for ERT. The problem of antigenicity due to differences in N-glycan structures between mammalian and yeast glycoproteins was potentially resolved by using alpha-1,6-mannosyltransferase-deficient (och1Delta) yeast as the host. Genes encoding the alpha- and beta-subunits of HexA were integrated into the yeast cell, and the heterodimer was expressed together with its isozymes HexS (alphaalpha) and HexB (betabeta). A total of 57 mg of beta-hexosaminidase isozymes, of which 13 mg was HexA (alphabeta), was produced per liter of medium. HexA was purified with immobilized metal affinity column for the His tag attached to the beta-subunit. The purified HexA was treated with alpha-mannosidase to expose mannose-6-phosphate (M6P) residues on the N-glycans. The specific activities of HexA and M6P-exposed HexA (M6PHexA) for the artificial substrate 4MU-GlcNAc were 1.2 +/- 0.1 and 1.7 +/- 0.3 mmol/h/mg, respectively. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern suggested a C-terminal truncation in the beta-subunit of the recombinant protein. M6PHexA was incorporated dose dependently into GM2 gangliosidosis patient-derived fibroblasts via M6P receptors on the cell surface, and degradation of accumulated GM2 ganglioside was observed.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Applied and environmental microbiology
- 巻
- 73
- 号
- 15
- 開始ページ
- 4805
- 終了ページ
- 12
- 出版年月
- 2007年8月
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0099-2240
- DOI URL
- 共同研究・競争的資金等の研究課題
研究者
田島 陽一
(タジマ ヨウイチ)