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論文
- タイトル
- タイトル（英）
- ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons.
- 参照URL
- https://researchmap.jp/hkawaji/published_papers/45603495
- 著者
- 著者（英）
- Takuya Kuroda,Satoshi Yasuda,Satoko Matsuyama,Takumi Miura,Rumi Sawada,Akifumi Matsuyama,Yumiko Yamamoto,Masaki Suimye Morioka,Hideya Kawaji,Takeya Kasukawa,Masayoshi Itoh,Hidenori Akutsu,Jun Kawai,Yoji Sato
- 担当区分
- 概要
- 概要（英）
- Despite the development of various in vitro differentiation protocols for the efficient derivation of specific cell types, human induced pluripotent stem cell (hiPSC) lines have varing ability to differentiate into specific lineages. Therefore, surrogate markers for accurately predicting the differentiation propensity of hiPSC lines may facilitate cell-based therapeutic product development and manufacture. We attempted to identify marker genes that could predict the differentiation propensity of hiPSCs into neural stem/progenitor cells (NS/PCs). Using Spearman's rank correlation coefficients, we investigated genes in the undifferentiated state, the expression levels of which were significantly correlated with the neuronal differentiation propensity of several hiPSC lines. Among genes significantly correlated with NS/PC differentiation (P < 0.01), we identified ROR2 as a novel predictive marker. ROR2 expression in hiPSCs was negatively correlated with NS/PC differentiation tendency, regardless of the differentiation method, whereas its knockdown enhanced differentiation. ROR2 regulates NS/PC differentiation, suggesting that ROR2 is functionally essential for NS/PC differentiation. Selecting cell lines with relatively low ROR2 expression facilitated identification of hiPSCs that can differentiate into NS/PCs. Cells with ROR2 knockdown showed increased efficiency of differentiation into forebrain GABAergic neurons compared to controls. These findings suggest that ROR2 is a surrogate marker for selecting hiPSC lines appropriate for NS/PC and GABAergic neuronal differentiations.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Scientific reports
- 巻
- 14
- 号
- 1
- 開始ページ
- 690
- 終了ページ
- 690
- 出版年月
- 2024年1月6日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1038/s41598-023-51082-4
- 共同研究・競争的資金等の研究課題
研究者
川路 英哉
( )