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論文
- タイトル
- タイトル（英）
- Formation and Long-Term Culture of hiPSC-Derived Sensory Nerve Organoids Using Microfluidic Devices
- 参照URL
- https://researchmap.jp/kazutakaikeda/published_papers/47319476
- 著者
- 著者（英）
- Takuma Ogawa,Souichi Yamada,Shuetsu Fukushi,Yuya Imai,Jiro Kawada,Kazutaka Ikeda,Seii Ohka,Shohei Kaneda
- 担当区分
- 概要
- 概要（英）
- Although methods for generating human induced pluripotent stem cell (hiPSC)-derived motor nerve organoids are well established, those for sensory nerve organoids are not. Therefore, this study investigated the feasibility of generating sensory nerve organoids composed of hiPSC-derived sensory neurons using a microfluidic approach. Notably, sensory neuronal axons from neurospheres containing 100,000 cells were unidirectionally elongated to form sensory nerve organoids over 6 mm long axon bundles within 14 days using I-shaped microchannels in microfluidic devices composed of polydimethylsiloxane (PDMS) chips and glass substrates. Additionally, the organoids were successfully cultured for more than 60 days by exchanging the culture medium. The percentage of nuclei located in the distal part of the axon bundles (the region 3−6 mm from the entrance of the microchannel) compared to the total number of cells in the neurosphere was 0.005% for live cells and 0.008% for dead cells. Molecular characterization confirmed the presence of the sensory neuron marker ISL LIM homeobox 1 (ISL1) and the capsaicin receptor transient receptor potential vanilloid 1 (TRPV1). Moreover, capsaicin stimulation activated TRPV1 in organoids, as evidenced by significant calcium ion influx. Conclusively, this study demonstrated the feasibility of long-term organoid culture and the potential applications of sensory nerve organoids in bioengineered nociceptive sensors.
- 出版者・発行元
- 出版者・発行元（英）
- MDPI AG
- 誌名
- 誌名（英）
- Bioengineering
- 巻
- 11
- 号
- 8
- 開始ページ
- 794
- 終了ページ
- 794
- 出版年月
- 2024年8月5日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.3390/bioengineering11080794
- 共同研究・競争的資金等の研究課題