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論文
- タイトル
- タイトル（英）
- Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.
- 参照URL
- https://researchmap.jp/toyonatsume/published_papers/47482313
- 著者
- 著者（英）
- Kayo Hibino,Yuji Sakai,Sachiko Tamura,Masatoshi Takagi,Katsuhiko Minami,Toyoaki Natsume,Masa A Shimazoe,Masato T Kanemaki,Naoko Imamoto,Kazuhiro Maeshima
- 担当区分
- 概要
- 概要（英）
- For accurate mitotic cell division, replicated chromatin must be assembled into chromosomes and faithfully segregated into daughter cells. While protein factors like condensin play key roles in this process, it is unclear how chromosome assembly proceeds as molecular events of nucleosomes in living cells and how condensins act on nucleosomes to organize chromosomes. To approach these questions, we investigate nucleosome behavior during mitosis of living human cells using single-nucleosome tracking, combined with rapid-protein depletion technology and computational modeling. Our results show that local nucleosome motion becomes increasingly constrained during mitotic chromosome assembly, which is functionally distinct from condensed apoptotic chromatin. Condensins act as molecular crosslinkers, locally constraining nucleosomes to organize chromosomes. Additionally, nucleosome-nucleosome interactions via histone tails constrain and compact whole chromosomes. Our findings elucidate the physical nature of the chromosome assembly process during mitosis.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Nature communications
- 巻
- 15
- 号
- 1
- 開始ページ
- 7152
- 終了ページ
- 7152
- 出版年月
- 2024年8月21日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1038/s41467-024-51454-y
- 共同研究・競争的資金等の研究課題
研究者
夏目 豊彰
(ナツメ トヨアキ)