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論文
- タイトル
- タイトル（英）
- TRAF7 determines circadian period through ubiquitination and degradation of DBP.
- 参照URL
- https://researchmap.jp/n_kurabayashi/published_papers/48153973
- 著者
- 著者（英）
- Shusaku Masuda,Nobuhiro Kurabayashi,Rina Nunokawa,Yuta Otobe,Hiroko Kozuka-Hata,Masaaki Oyama,Yuri Shibata,Jun-Ichiro Inoue,Michinori Koebis,Atsu Aiba,Hikari Yoshitane,Yoshitaka Fukada
- 担当区分
- 筆頭著者
- 概要
- 概要（英）
- D-site binding protein, DBP, is a clock-controlled transcription factor and drives daily rhythms of physiological processes through the regulation of an array of genes harboring a DNA binding motif, D-box. DBP protein levels show a circadian oscillation with an extremely robust peak/trough ratio, but it is elusive how the temporal pattern is regulated by post-translational regulation. In this study, we show that DBP protein levels are down-regulated by the ubiquitin-proteasome pathway. Analysis using 19 dominant-negative forms of E2 enzymes have revealed that UBE2G1 and UBE2T mediate the degradation of DBP. A proteomic analysis of DBP-interacting proteins and database screening have identified Tumor necrosis factor Receptor-Associated Factor 7 (TRAF7), a RING-type E3 ligase, that forms a complex with UBE2G1 and/or UBE2T. Ubiquitination analysis have revealed that TRAF7 enhances K48-linked polyubiquitination of DBP in cultured cells. Overexpression of TRAF7 down-regulates DBP protein level, while knockdown of TRAF7 up-regulates DBP in cultured cells. Knockout of TRAF7 in NIH3T3 cells have revealed that TRAF7 mediates the time-of-the-day-dependent regulation of DBP levels. Furthermore, TRAF7 has a period-shortening effect on the cellular clock. Together, TRAF7 plays an important role in circadian clock oscillation through destabilization of DBP.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Communications biology
- 巻
- 7
- 号
- 1
- 開始ページ
- 1280
- 終了ページ
- 1280
- 出版年月
- 2024年10月8日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1038/s42003-024-07002-x
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