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論文
- タイトル
- タイトル（英）
- Homology recognition without double-stranded DNA-strand separation in D-loop formation by RecA
- 参照URL
- https://researchmap.jp/7000009079/published_papers/49496969
- 著者
- 著者（英）
- Takehiko Shibata,Shukuko Ikawa,Wakana Iwasaki,Hiroyuki Sasanuma,Hisao Masai,Kouji Hirota
- 担当区分
- 概要
- 概要（英）
- Abstract RecA protein and RecA/Rad51 orthologues are required for homologous recombination and DNA repair in all living creatures. RecA/Rad51 catalyzes formation of the D-loop, an obligatory recombination intermediate, through an ATP-dependent reaction consisting of two phases: homology recognition between double-stranded (ds)DNA and single-stranded (ss)DNA to form a hybrid-duplex core of 6–8 base pairs and subsequent hybrid-duplex/D-loop processing. How dsDNA recognizes homologous ssDNA is controversial. The aromatic residue at the tip of the β-hairpin loop (L2) was shown to stabilize dsDNA-strand separation. We tested a model in which dsDNA strands were separated by the aromatic residue before homology recognition and found that the aromatic residue was not essential to homology recognition, but was required for D-loop processing. Contrary to the model, we found that the double helix was not unwound even a single turn during search for sequence homology, but rather was unwound only after the homologous sequence was recognized. These results suggest that dsDNA recognizes its homologous ssDNA before strand separation. The search for homologous sequence with homologous ssDNA without dsDNA-strand separation does not generate stress within the dsDNA; this would be an advantage for dsDNA to express homology-dependent functions in vivo and also in vitro.
- 出版者・発行元
- 出版者・発行元（英）
- Oxford University Press (OUP)
- 誌名
- 誌名（英）
- Nucleic Acids Research
- 巻
- 52
- 号
- 5
- 開始ページ
- 2565
- 終了ページ
- 2577
- 出版年月
- 2024年1月12日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0305-1048
- DOI URL
- https://doi.org/10.1093/nar/gkad1260
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