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論文
- タイトル
- タイトル（英）
- Segment-specific promoter activity for RNA synthesis in the genome of Oz virus, genus Thogotovirus.
- 参照URL
- https://researchmap.jp/matsumotyu/published_papers/48974362
- 著者
- 著者（英）
- Lipi Akter,Ryo Matsumura,Daisuke Kobayashi,Hiromichi Matsugo,Haruhiko Isawa,Yusuke Matsumoto
- 担当区分
- 責任著者
- 概要
- 概要（英）
- Oz virus (OZV), a tick-borne, six-segmented negative-strand RNA virus in the genus Thogotovirus, caused a fatal human infection in Japan in 2023. To study viral RNA synthesis, we developed an OZV minigenome assay using mammalian cells. This revealed variations in promoter activities among the six genome segments. The "distal duplex," a double-stranded RNA structure beginning at the 11th nucleotide on the 5' end and the 10th on the 3' end, was found in all segments. A factor affecting promoter activity was the base pairing between the 12th nucleotide at the 5' end and the 11th at the 3' end, forming either G:C or A:U pairs. Disruption of this pairing caused a significant loss of promoter activity, emphasizing the importance of the distal duplex with at least six consecutive base pairs. Comparative analysis of genome terminal sequences suggests similar structural variations in the promoters of other species in Thogotovirus.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Virology
- 巻
- 603
- 号
- 開始ページ
- 110410
- 終了ページ
- 110410
- 出版年月
- 2025年1月15日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.virol.2025.110410
- 共同研究・競争的資金等の研究課題
研究者
松本 祐介
( )