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論文
- タイトル
- タイトル（英）
- Astrocytes functionally integrate multiple synapses via specialized leaflet domains.
- 参照URL
- https://researchmap.jp/Chihi/published_papers/51980270
- 著者
- 著者（英）
- Lucas Benoit,Ines Hristovska,Nicolas Liaudet,Pierre-Henri Jouneau,Arnold Fertin,Roberta de Ceglia,David G Litvin,Maria Amalia Di Castro,Milica Jevtic,Ioannis Zalachoras,Toko Kikuchi,Ludovic Telley,Matteo Bergami,Yves Usson,Chihiro Hisatsune,Katsuhiko Mikoshiba,Karin Pernet-Gallay,Andrea Volterra
- 担当区分
- 概要
- 概要（英）
- Astrocyte Ca2+ dynamics control synaptic circuits and behavior, yet the underlying biology remains poorly understood. By combining volumetric high-resolution electron microscopy and two-photon Ca2+ imaging, we characterize astrocyte leaflets that interface with synapses. These convoluted structures with ≤250 nm diameter originate from astrocytic shafts or cell bodies, contain minuscule endoplasmic reticulum saccules expressing IP3 receptors but not mitochondria, and are often interconnected via gap junctions forming domains with cytosolic continuity. Leaflets enwrap 90% of synapses in clusters and only 10% individually. By fast imaging of astrocyte peripheral microvolumes, we identify leaflet-specific Ca2+ events that were synaptically induced, IP3R1-mediated, and often displayed separate originations merging into large, long-lasting Ca2+ elevations. Using combined axon-leaflet Ca2+ imaging, we show that these complex events reflect integration of incoming inputs from different neurons. The astrocyte leaflet organization may thus coordinate, via Ca2+ signals, multiple synapses and circuits active at different spatiotemporal scales, executing computations distinct from neurons.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Cell
- 巻
- 188
- 号
- 23
- 開始ページ
- 6453
- 終了ページ
- 6472
- 出版年月
- 2025年11月13日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.cell.2025.08.036
- 共同研究・競争的資金等の研究課題
研究者
久恒 智博
( )