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論文
- タイトル
- Electron Microscopic Analysis of α-Synuclein Fibrils.
- タイトル（英）
- Electron Microscopic Analysis of α-Synuclein Fibrils.
- 参照URL
- https://researchmap.jp/masatohasegawa/published_papers/33626241
- 著者
- 著者（英）
- Airi Tarutani,Masato Hasegawa
- 担当区分
- 概要
- 概要（英）
- α-Synuclein (α-syn) is a major component of abnormal protein deposits observed in the brains of patients with synucleinopathies, including Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy (MSA). The synaptic protein α-syn is water-soluble under normal physiological conditions, but in these patients' brains, we see accumulation of insoluble amyloid-like α-syn fibrils with prion-like properties. Intracerebral accumulation of these fibrils is correlated with disease onset and progression. Recombinant α-syn protein also forms amyloid-like fibrils that are structurally akin to those extracted from patients' brains. Recent cryo-electron microscopic studies have identified the core structures of synthetic α-syn fibrils and α-syn fibrils extracted from the brains of patients with MSA at the atomic level. In this chapter, we describe negative staining and immunoelectron microscopy protocols for ultrastructural characterization of synthetic α-syn fibrils and pathological α-syn fibrils.
- 出版者・発行元
- 出版者・発行元（英）
- Springer US
- 誌名
- 誌名（英）
- Methods in Molecular Biology
- 巻
- 2322
- 号
- 開始ページ
- 17
- 終了ページ
- 25
- 出版年月
- 2021年
- 査読の有無
- 査読有り
- 招待の有無
- 招待有り
- 掲載種別
- 論文集(書籍)内論文
- ISSN
- 1064-3745
- DOI URL
- https://doi.org/10.1007/978-1-0716-1495-2_2
- 共同研究・競争的資金等の研究課題
研究者
Hasegawa Masato
( )