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論文
- タイトル
- タイトル（英）
- Caffeine promotes glutamate and histamine release in the posterior hypothalamus
- 参照URL
- https://researchmap.jp/SleepLab/published_papers/28064181
- 著者
- 著者（英）
- Joshi John,Tohru Kodama,Jerome M. Siegel
- 担当区分
- 概要
- 概要（英）
- Histamine neurons are active during waking and largely inactive during sleep, with minimal activity during rapid-eye movement (REM) sleep. Caffeine, the most widely used stimulant, causes a significant increase of sleep onset latency in rats and humans. We hypothesized that caffeine increases glutamate release in the posterior hypothalamus (PH) and produces increased activity of wake-active histamine neurons. Using in vivo microdialysis, we collected samples from the PH after caffeine administration in freely behaving rats. HPLC analysis and biosensor measurements showed a significant increase in glutamate levels beginning 30 min after caffeine administration. Glutamate levels remained elevated for at least 140 min. GABA levels did not significantly change over the same time period. Histamine level significantly increased beginning 30 min after caffeine administration and remained elevated for at least 140 min. Immunostaining showed a significantly elevated number of c-Fos-labeled histamine neurons in caffeine-treated rats compared with saline-treated animals. We conclude that increased glutamate levels in the PH activate histamine neurons and contribute to caffeine-induced waking and alertness.
- 出版者・発行元
- 出版者・発行元（英）
- AMER PHYSIOLOGICAL SOC
- 誌名
- 誌名（英）
- AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
- 巻
- 307
- 号
- 6
- 開始ページ
- R704
- 終了ページ
- R710
- 出版年月
- 2014年9月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0363-6119
- DOI URL
- https://doi.org/10.1152/ajpregu.00114.2014
- 共同研究・競争的資金等の研究課題
研究者
児玉 亨
( )