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論文
- タイトル
- タイトル（英）
- Genome Editing Is Induced in a Binary Manner in Single Human Cells
- 参照URL
- https://researchmap.jp/YuichiroMiyaoka/published_papers/36498903
- 著者
- 著者（英）
- Gou Takahashi,Daiki Kondo,Minato Maeda,Yuji Morishita,Yuichiro Miyaoka
- 担当区分
- 概要
- 概要（英）
Abstract Even when precise nucleotide manipulations are intended, the outcomes of genome editing can be diverse, often including random insertions and deletions. The combinations and frequencies of these different outcomes in single cells are critical not only in the generation of genetically modified cell lines but also in the evaluation of the clinical effects of genome editing therapies. However, current methods only analyze cell populations, not single cells. Here, we utilized the Single Particle isolation System (SPiS) for the efficient isolation of single cells to systematically analyze genome editing results in individual human cultured cells. As a result, we discovered that genome editing induction has a binary nature, that is, the target alleles of cells tend to be all edited or not edited at all. This study enhances our understanding of the induction mechanism of genome editing and provides a new strategy to analyze genome editing outcomes in single cells.- 出版者・発行元
- 出版者・発行元（英）
- Cold Spring Harbor Laboratory
- 誌名
- 誌名（英）
- 巻
- 号
- 開始ページ
- 終了ページ
- 出版年月
- 2022年3月5日
- 査読の有無
- 招待の有無
- 掲載種別
- ISSN
- DOI URL
- https://doi.org/10.1101/2022.03.04.482947
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