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論文
タイトル
タイトル(英)
Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer
参照URL
https://researchmap.jp/obokikeisuke/published_papers/37227459
著者
著者(英)
Ryo Negishi,Hitomi Yamakawa,Takeru Kobayashi,Mayuko Horikawa,Tatsu Shimoyama,Fumiaki Koizumi,Takeshi Sawada,Keisuke Oboki,Yasushi Omuro,Chikako Funasaka,Akihiko Kageyama,Yusuke Kanemasa,Tsuyoshi Tanaka,Tadashi Matsunaga,Tomoko Yoshino
概要
概要(英)
Abstract Transcriptome analysis of circulating tumor cells (CTCs), which migrate into blood vessels from primary tumor tissues, at the single-cell level offers critical insights into the biology of metastasis and contributes to drug discovery. However, transcriptome analysis of single CTCs has only been reported for a limited number of cancer types, such as multiple myeloma, breast, hepatocellular, and prostate cancer. Herein, we report the transcriptome analysis of gastric cancer single-CTCs. We utilized an antigen-independent strategy for CTC isolation from metastatic gastric cancer patients involving a size-dependent recovery of CTCs and a single cell isolation technique. The transcriptomic profile of single-CTCs revealed that a majority of gastric CTCs had undergone epithelial-mesenchymal transition (EMT), and indicated the contribution of platelet adhesion toward EMT progression and acquisition of chemoresistance. Taken together, this study serves to employ CTC characterization to elucidate the mechanisms of chemoresistance and metastasis in gastric cancer.
出版者・発行元
出版者・発行元(英)
Springer Science and Business Media LLC
誌名
誌名(英)
Communications Biology
5
1
開始ページ
終了ページ
出版年月
2022年12月
査読の有無
招待の有無
掲載種別
研究論文(学術雑誌)
ISSN
DOI URL
https://doi.org/10.1038/s42003-021-02937-x
共同研究・競争的資金等の研究課題
研究者
大保木 啓介 (オオボキ ケイスケ)