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論文
- タイトル
- タイトル（英）
- Effect of Ca2+ on vesicle fusion on solid surface: An in vitro model of protein-accelerated vesicle fusion
- 参照URL
- https://researchmap.jp/read0091842/published_papers/29793661
- 著者
- 著者（英）
- Youichi Shinozaki,Ari M. Sittonen,Koji Sumitomo,Kazuaki Furukawa,Keiichi Torimitsu
- 担当区分
- 概要
- 概要（英）
- Lipid vesicle fusion is an important reaction in the cell. Calcium ions (Ca2+) participate in various important biological events including the fusion of vesicles with cell membranes in cells. We studied the effect of Ca2+ on the fusion of egg yolk phosphatidylcholine/brain phosphatidylserine (eggPC/brainPS) lipid vesicles on a mica substrate with fast scanning atomic force microscopy (AFM). When unattached and unfused lipid vesicles on mica were rinsed away, discrete patches of fused vesicles were observed under high Ca2+ concentrations. At 0 mM Ca2+, lipid vesicles were fused on mica and formed continuous supported lipid bilayers (SLBs) covering almost the entire mica surface. The effect of Ca2+ on SLB formation was offset by a Ca2+ chelating agent. When lipid vesicles were added during AFM observation, vesicles fused on mica and covered almost all areas even under high Ca2+ concentrations. These results indicate that force between AFM tip and vesicles overcomes the Ca 2+-reduced fusion of lipid vesicles. © 2008 The Japan Society of Applied Physics.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Japanese Journal of Applied Physics
- 巻
- 47
- 号
- 7
- 開始ページ
- 6164
- 終了ページ
- 6167
- 出版年月
- 2008年7月18日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0021-4922,1347-4065
- DOI URL
- https://doi.org/10.1143/JJAP.47.6164
- 共同研究・競争的資金等の研究課題
研究者
篠﨑 陽一
(シノザキ ヨウイチ)