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論文
- タイトル
- タイトル（英）
- Impacts of an age-related hearing loss allele of cadherin 23 on severity of hearing loss in ICR and NOD/Shi mice.
- 参照URL
- https://researchmap.jp/glirulus/published_papers/43209217
- 著者
- 著者（英）
- Xuehan Hou,Shumpei P Yasuda,Midori Yamaguchi,Sari Suzuki,Yuta Seki,Takafumi Ouchi,Ting Mao,Ornjira Prakhongcheep,Hiroshi Shitara,Yoshiaki Kikkawa
- 担当区分
- 概要
- 概要（英）
- The age-related hearing loss allele (Cdh23ahl) of the cadherin 23 gene leads to a more severe hearing loss phenotype through additive effects with risk alleles for hearing loss. In this study, we genome edited the Cdh23ahl allele to the wild-type Cdh23+ allele in outbred ICR mice and inbred NOD/Shi mice established from ICR mice and investigated their effects on hearing phenotypes. Several hearing tests confirmed that ICR mice developed early onset high-frequency hearing loss and exhibited individual differences in hearing loss onset times. Severe loss of cochlear hair cells was also detected in the high-frequency areas in ICR mice. These phenotypes were rescued by genome editing the Cdh23ahl allele to Cdh23+, suggesting that abnormal hearing phenotypes develop because of the interaction of the Cdh23ahl and risk alleles in the genetic background of ICR mice. NOD/Shi mice developed more severe hearing loss and hair cell degeneration than ICR mice. Hearing loss was detected at 1 month old. Hair cell loss, including degeneration of cell bodies and stereocilia, was observed in all regions of the cochlea in NOD/Shi mice. Although these phenotypes were partially rescued by genome editing to the Cdh23+ allele, the phenotypes associated with high-frequency hearing were mostly unrecovered in NOD/Shi mice. These results strongly suggest that the genetic background of NOD/Shi mice contain a potential risk allele for the acceleration of early onset high-frequency hearing loss.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Biochemical and biophysical research communications
- 巻
- 674
- 号
- 開始ページ
- 147
- 終了ページ
- 153
- 出版年月
- 2023年9月24日
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1016/j.bbrc.2023.07.003
- 共同研究・競争的資金等の研究課題