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論文
- タイトル
- タイトル（英）
- Glycoconjugate microarray based on an evanescent-field fluorescence-assisted detection principle for investigation of glycan-binding proteins
- 参照URL
- https://researchmap.jp/read0134472/published_papers/7251879
- 著者
- 著者（英）
- Hiroaki Tateno,Atsushi Mori,Noboru Uchiyama,Rikio Yabe,Jun Iwaki,Toshihide Shikanai,Takashi Angata,Hisashi Narimatsu,Jun Hirabayashi
- 担当区分
- 概要
- 概要（英）
- The extensive involvement of glycan-binding proteins (GBPs) as regulators in diverse biological phenomena provides a fundamental reason to investigate their glycan-binding specificities. Here, we developed a glycoconjugate microarray based on an evanescent-field fluorescence-assisted detection principle for investigation of GBPs. Eighty-nine selected multivalent glycoconjugates comprising natural glycoproteins, neo-glycoproteins, and polyacrylamide (PAA)-conjugated glycan epitopes were immobilized on an epoxy-activated glass slide. The GBP binding was monitored by an evanescent-field fluorescence-assisted scanner at equilibrium without washing steps. The detection principle also allows direct application of unpurified GBPs with the aid of specific antibodies. Model experiments using plant lectins (RCA120, ConA, and SNA), galectins (3 and 8), a C-type lectin (DC-SIGN) and a siglec (CD22) provided data consistent with previous work within 4 h using less than 40 ng of GBPs per analysis. As an application, serum profiling of antiglycan antibodies (IgG and IgM) was performed with Cy3-labeled secondary antibodies. Moreover, novel carbohydrate-binding ability was demonstrated for a human IL-18 binding protein. Thus, the developed glycan array is useful for investigation of various types of GBPs, with the added advantage of wash-free analysis.
- 出版者・発行元
- 出版者・発行元（英）
- OXFORD UNIV PRESS INC
- 誌名
- 誌名（英）
- GLYCOBIOLOGY
- 巻
- 18
- 号
- 10
- 開始ページ
- 789
- 終了ページ
- 798
- 出版年月
- 2008年10月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 0959-6658
- DOI URL
- https://doi.org/10.1093/glycob/cwn068
- 共同研究・競争的資金等の研究課題
研究者
矢部 力朗
(ヤベ リキオ)