K. Miyashita*, T. Yagi*, N. Kagaya, A. Takechi, C. Nakata, R. Kanda, H. Nuriya, K. Tanegashima, S. Hoyano, F. Seki, C. Yoshida, Y. Hachiro, T. Higashi, N. Kitada, T. Toya, T. Kobayashi, Y. Najima, S. Goyama, S. A. Maki, T. Kitamura, N. Doki, K. Shin-ya, and T. Hara. Identification of compounds that preferentially suppress the growth of T cell acute lymphoblastic leukemia-derived cells. Cancer Sci., 114: 4032-4040, 2023. *First two authors equally contributed to the work.
T. Higashi, C. Yoshida, Y. Hachiro, C. Nakata, A. Takechi, T. Yagi, K. Miyashita, N. Kitada, R. Obata, T. Hirano, T. Hara, and S. A Maki. Synthesis and anti-tumor activities in human leukemia-derived cells of polyenylpyrroles with a methyl group at the conjugated polyene terminus. Bioorg. Med. Chem. Lett., 95: 129471, 2023.
K. Kitajima*, M. Shingai, H. Ando, M. Hamasaki, and T. Hara*. An interferon-γ/FLT3 axis positively regulates hemopoietic progenitor cell expansion from human pluripotent stem cells. Stem Cells, 40: 906-918, 2022. *Co-corresponding authors.
K. Tsujihana*, K. Tanegashima*, Y. Santo, H. Yamada, S. Akazawa, R. Nakao, K. Tominaga, R. Saito, Y. Nishito, R. Hata, T. Nakamura, I. Murai, Y. Kono, M. Sugawa, M. Tanioka, G. Egawa, M. Doi, T. Isa, K. Kabashima, T. Hara**, and H. Okamura**. Circadian protection against bacterial skin infection by epidermal CXCL14-mediated innate immunity. Proc. Natl. Acad. Sci. U.S.A., 119: e2116027119, 2022. *Equally contributed authors, **Co-corresponding authors.
T. Suzuki, S. Takagi, and T. Hara. Multiple gene transfer and all-in-one conditional knockout systems in mouse embryonic stem cells for analysis of gene function. Front. Cell Dev. Biol., 10: 870629, 2022.
2021年
R. Iwase, N. Naruse, M. Nakagawa, R. Saito, A. Shigenaga, A. Otaka, T. Hara, and K. Tanegashima. Identification of functional domains of CXCL14 involved in high-affinity binding and intracellular transport of CpG DNA. J. Immunol., 207: 459-469, 2021.
T. Suzuki, E. Katada, Y. Mizuoka, S. Takagi, Y. Kazuki, M. Oshimura, M. Shindo, and T. Hara. A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing. Nucl. Acid Res, 49: e40, 2021.
C. Yoshida, T. Higashi, Y. Hachiro, Y. Fujita, T. Yagi, A. Takechi, C. Nakata, K. Miyashita, N. Kitada, R. Saito, R. Obata, T. Hirano, T. Hara, and S. A. Maki. Synthesis of polyenylpyrrole derivatives with selective growth inhibitory activity against T-cell acute lymphoblastic leukemia cells. Bioorg. Med. Chem. Lett., 30: 127837, 2021.
2020年
K. Sato, M. Maeda, E. Kamata, S. Ishii, K. Yanagisawa, K. Kitajima, and T. Hara. Nitric oxide and a conditioned medium affect the hematopoietic development in a microfluidic mouse embryonic stem cell/OP9 co-cultivation system. Micromachines, 11: 305, 2020.
M. Paulis, L. Susani, A. Castelli, T. Suzuki, T. Hara, L. Straniero, S. Duga, D. Strina, S. Mantero, E. Caldana, L. S. Sergi, A. Villa, and P. Vezzoni. Chromosome transplantation: a possible approach to treat human X-linked disorders. Mol. Ther. Methods Clin. Dev., 17: 369-377, 2020.
T. Suzuki, Y. Kazuki, T. Hara, and M. Oshimura. Current advances in microcell-mediated chromosome transfer technology and its applications. Exp. Cell Res., 390: 111915, 2020.
鈴木輝彦、原 孝彦:染色体導入法の原理と技術改良. 細胞, 52: 408-411, 2020.
2019年
M. Nakajima, T. Suzuki, T. Hara, and K. Kitajima. In vitro differentiation of mouse T cell-derived hybrid cells obtained through cell fusion with embryonic stem cells. Biochem. Biophys. Res. Commun., 513: 701-707, 2019.
H. Tabata, T. Hara, and K. Kitajima. Inhibitory action of an ERK1/2 inhibitor on primitive endoderm cell differentiation from mouse embryonic stem cells. Biochem. Biophys. Res. Commun., 512: 399-404, 2019.
N. Yokote, M. Y. Suzuki-Kosaka, T. Michiue, T. Hara, and K. Tanegashima. Latrophilin2 is involved in neural crest cell migration and placode patterning in Xenopus laevis. Int. J. Dev. Biol., 63: 29-35, 2019.
2018年
K. Kitajima, M. Kanokoda, M. Nakajima, and T. Hara. Domain-specific biological functions of the transcription factor Gata2 on hematopoietic differentiation of mouse embryonic stem cells. Genes Cells, 23: 753-766, 2018.
K. Tanaka, N. Ikeda, K. Miyashita, H. Nuriya, and T. Hara. DEAD box protein DDX1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene. Cancer Sci., 109: 2479-2489, 2018.
K. Miyashita, K. Kitajima, S. Goyama, T. Kitamura, and T. Hara. Overexpression of Lhx2 suppresses proliferation of human T cell acute lymphoblastic leukemia-derived cells, partly by reducing LMO2 protein levels. Biochem. Biophys. Res. Commun., 495: 2310-2316, 2018.
2017年
K. Tanegashima, R. Takahashi, H. Nuriya, R. Iwase, N. Naruse, K. Tsuji, A. Shigenaga, A. Otaka, and T. Hara. CXCL14 acts as a specific carrier of CpG DNA into dendritic cells and activates Toll-like receptor 9-mediated adaptive immunity. EBioMedicine, 24: 247-256, 2017.
T. Shinohara, K.Kazuki, N. Ogonuki, H. Morimoto, S. Matoba, K. Hiramatsu, K. Honma, T. Suzuki, T. Hara, A. Ogura, M. Oshimura, M. Kanatsu-Shinohara, and Y. Kazuki. Transfer of mouse artificial chromosome into spermatogonial stem cells generates transchromosomic mice. Stem Cell Rep., 9: 1180-1191, 2017.
K. Tanegashima, Y. Sato-Miyata, M. Funakoshi, Y. Nishito, T. Aigaki, and T. Hara. Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice. Genes to Cells, 22: 71-83, 2017.
H. Ohtsuka, T. Iguchi, M. Hayashi, M. Kaneda, K. Iida, M. Shimonaka, T. Hara, M. Arai, Y. Koike, N. Yamamoto, and K. Kasahara. SDF-1α/CXCR4 signaling in lipid rafts induces platelet aggregation via PI3 kinase-dependent Akt phosphorylation. PLoS ONE, 12: e0169609, 2017.
2016年
T. Suzuki, Y. Kazuki, M. Oshimura, and T. Hara. Highly efficient transfer of chromosomes to a broad range of target cells using Chinese hamster ovary cells expressing murine leukemia virus-derived envelope proteins. PLoS ONE, 11: e0157187, 2016.
K. Tamura, H. Naraba, T. Hara, K. Nakamura, M. Yoshie, H. Kogo, and E. Tachikawa. A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells. Prostaglandins Other Lipid Mediat., 123: 56-62, 2016.
M. Kawaguchi, K. Kitajima, M. Kanokoda, H. Suzuki, K. Miyashita, M. Nakajima, H. Nuriya, K. Kasahara, and T. Hara. Efficient production of platelets from mouse embryonic stem cells by enforced expression of Gata2 in late hemogenic endothelial cells. Biochem. Biophys. Res. Commun., 474: 462-468, 2016.
K. Kitajima, M. Nakajima, M. Kanokoda, M. Kyba, A. Dandapat, J. Tolar, M. K. Saito, M. Toyoda, A. Umezawa, and T. Hara. GSK3β inhibition activates the CDX/HOX pathway and promotes hemogenic endothelial progenitor differentiation from human pluripotent stem cells. Exp. Hematol., 44: 68-74, 2016.
2015年
K. Tsuji, K. Tanegashima, K. Sato, K. Sakamoto, A. Shigenaga, T. Inokuma, T. Hara*, and A. Otaka*. Efficient one-pot synthesis of CXCL14 and its derivative using an N-sulfanylethylanilide peptide as a peptide thioester equivalent and thier biological evaluation. Bioorg. Med. Chem., 23: 5909-5914, 2015. *Co-corresponding author.
K. Kitajima, M. Kawaguchi, K. Miyashita, M. Nakajima, M. Kanokoda, and T. Hara. Efficient production of T cells from mouse pluripotent stem cells by controlled expression of Lhx2. Genes to Cells, 20: 720-738, 2015.
Y. Kodaka, K. Tanaka, K. Kitajima, K. Tanegashima, R. Matsuda, and T Hara. LIM homeobox transcription factor Lhx2 inhibits skeletal muscle differentiation in part via transcriptional activation of Msx1 and Msx2. Exp. Cell Res., 331: 309-319, 2015.
2014年
T. Suzuki, Y. Kazuki, M. Oshimura, and T. Hara. A novel system for simultaneous or sequential integration of multiple gene-loading vectors into a defined site of a human artificial chromosome. PLoS ONE, 9: e110404, 2014.
T. Hara and K. Tanegashima. CXCL14 antagonizes the CXCL12-CXCR4 signaling axis. BioMol. Concepts 5: 167-173, 2014.
K. Kitajima, M. Kawaguch, M. Iacovino, M. Kyba, and T. Hara. Molecular functions of the LIM-homeobox transcription factor Lhx2 in hematopoietic progenitor cells derived from mouse embryonic stem cells. Stem Cells, 31: 2680-2689, 2013.
K. Tanegashima, K. Tsuji, K Suzuki, A. Shigenaga, A. Otaka, and T. Hara. Dimeric peptides of the C-terminal region of CXCL14 function as CXCL12 inhibitors. FEBS Letters, 587: 3770-3775, 2013.
K. Tanaka, K. Kondo, K. Kitajima, M. Muraoka, A. Nozawa, and T. Hara. Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA. J. Biol. Chem., 288: 23990-23999, 2013.
K. Tanegashima, K Suzuki, Y. Nakayama, K. Tsuji, A. Shigenaga, A. Otaka, and T. Hara. CXCL14 is a natural inhibitor of the CXCL12-CXCR4 signaling axis. FEBS Letters, 587: 1731-1735, 2013.
2012年
T. Hara and K. Tanegashima. Pleiotropic functions of the CXC-type chemokine CXCL14 in mammals. J. Biochem., 151: 469-476, 2012.
A. Ishimura, K. Minehata, M. Terashima, G. Kondoh, T. Hara, and T. Suzuki. Jmjd5, an H3K36me2 histone demethylase, modulates embryonic cell proliferation through the regulation of Cdkn1a expression. Development, 139: 749-759, 2012.
Z. Zhao, L. Qin, B. Reid, J. Pu, T. Hara, and M. Zhao. Directing migration of endothelial progenitor cells with applied DC electric fields. Stem Cell Res., 8: 38-48, 2012.
K. Kitajima, K. Minehata, K. Sakimura, T. Nakano, and T. Hara. In vitro generation of HSC-like cells from murine ESCs/iPSCs by enforced expression of LIM-homeobox transcription factor Lhx2. Blood, 117: 3748-3758, 2011.
T. Hara. Thymosins and muscle regeneration. Vitamins and Hormones, 87: 277-290, 2011.
K. Tsuji, A. Shigenaga, Y. Sumikawa, K. Tanegashima, K. Sato, K. Aihara, T. Hara, and A. Otaka. Application of N to C- or C to N-directed sequential native chemical ligation to the preparation of CXCL14 analogs and their biological evaluation. Bioorg. Med. Chem., 19: 4014-4020, 2011.
K. Tatsumi, H. Yamamoto-Mukai, R. Shimizu, S. Waguri, Y.-S. Sou, A. Sakamoto, C. Taya, H. Shitara, T. Hara, C. H. Chung, K. Tanaka, M. Yamamoto, and M. Komatsu. The Ufm1-activating enzyme Uba5 is indispensable for erythroid differentiation in mice. Nat. Commun., 2: 181, 2011. (doi:10.1038/ncomms1182)
Y. Tokura, Y. Nakayama, S. Fukada, N. Nara, H. Yamamoto, R. Matsuda, and T. Hara. Muscle injury-induced thymosin beta4 acts as a chemoattractant for myoblasts. J. Biochem., 149: 43-48, 2011.
T. Hara and K. Tanaka. DDX1 [DEAD (Asp-Glu-Ala-Asp) box polypeptide 1]. Atlas Genet. Cytogenet. Oncol. Haematol., URL: http://AtlasGeneticsOncology.org/Genes/DDX1ID40283ch2p24.html, June 2010.
K. Ohbayashi, K. Tanaka, K. Kitajima, K. Tamura, and T. Hara. Novel role for the intraflagellar transport protein CMG-1 in regulating the transcription of cyclin-D2, E-cadherin, and integrin-alpha family genes in mouse spermatocyte-derived cells. Genes to Cells.,15: 699-710, 2010.
K. Tanegashima, S. Okamoto, Y. Nakayama, C. Taya, H. Shitara, R. Ishii, H. Yonekawa, Y. Minokoshi, and T. Hara. CXCL14 deficiency in mice attenuates obesity and inhibits feeding behavior in a novel environment. PLoS ONE, 5: e10321, 2010.
K. Tanegashima, K. Suzuki, Y. Nakayama, and T. Hara. Antibody-assisted enhancement of biological activities of CXCL14 in human monocytic leukemia-derived THP-1 cells and high fat diet-induced obese mice. Exp. Cell Res., 316: 1263-1270, 2010.
K. Tanaka, S. Okamoto, Y. Ishikawa, H. Tamura, and T. Hara. DDX1 is required for testicular tumorigenesis, partially through the transcriptional activation of 12p stem cell genes. Oncogene, 28: 2142-2151, 2009.
T. Hara and Y. Nakayama. CXCL14 and insulin action. Vitamins and Hormones 80: 107-123, 2009.
S. Ohata, M. Nawa, T. Kasama, T. Yamasaki, K. Sawanoboria, S. Hata, T. Nakamura, Y. Asaoka, T. Watanabe, H. Okamoto, T. Hara, S. Terai, I. Sakaida, T. Katada, and H. Nishina. Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. Biochem. Biophys. Res. Commun., 379: 817-823, 2009.
2008年
M. Kutsukake, R. Ishihara, K. Momose, K. Isaka, O. Itokazu, C. Higuma, T. Matsutani, A. Matsuda , A. Sasajima, T. Hara, and K. Tamura. Circulating IGF-binding protein 7 (IGFBP7) levels are elevated in patients with endometriosis or undergoing diabetic hemodialysis. Reprod. Biol. Endocrinol., 6: 54, 2008.
T. Hara*, M. Schwieger, R. Kazama, S. Okamoto, K. Minehata, M. Ziegler, J. Lohler, and C. Stocking. Acceleration of chronic myeloproliferation by enforced expression of Meis1 or Meis3 in Icsbp-deficient bone marrow cells. Oncogene, 27: 3865-3869, 2008. *Corresponding author.
N. Nara, Y. Nakayama, S. Okamoto, H. Tamura, M. Kiyono, M. Muraoka, K. Tanaka, C. Taya, H. Shitara, R. Ishii, H. Yonekawa, Y. Minokoshi, and T. Hara. Disruption of CXC motif chemokine ligand-14 in mice ameliorates obesity-induced insulin resistance. J. Biol. Chem., 282: 30794-30803, 2007.
M. Muraoka*, T. Miki, N. Ishida, T. Hara, and M. Kawakita. Variety of nucleotide sugar transporters with respect to the interaction wtih nucleoside mono- and diphosphates. J. Biol. Chem., 282: 24615-24622, 2007. *Corresponding author.
K. Tamura, M. Yoshie, T. Hara, K. Isaka, and H. Kogo. Involvement of stathmin in the proliferation and differentiation of immortalized human endometrial stromal cells. J. Reprod. Dev. , 53: 525-533, 2007.
E. Itakura, K. Odaira, K. Yokoyama, M. Osuna, T. Hara, and K. Inoue. Generation of transgenic rats expressing green fluorescent protein in S-100b-producing pituitary folliculo-stellate cells and brain astrocytes. Endocrinol., 148: 1518-1523, 2007.
L. Cao, H. Shitara, T. Horii, Y. Nagao, H. Imai, K. Abe, T. Hara, J. Hayashi, and H. Yonekawa. Mitochondrial bottleneck occurs without reduction of mtDNA content in mouse female germ cells. Nat. Genet., 39: 386-390, 2007.
2006年
K. Tamura, M. Yoshie, H. Nishi, Y. Osakabe, K. Isaka, T. Hara, and H. Kogo. Expression of stathmin in human uterus and decidualizing endometrial stromal cells. Reproduction, 132: 625-635, 2006.
T. Suzuki, Y. Kanai, T. Hara, J. Sasaki, T. Sasaki, M. Kohara, T. Maehama, C. Taya, H. Shitara, H. Yonekawa, M. A. Frohman, T. Yokozeki, and Y. Kanaho. Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development. Mol. Cell Biol., 26: 6149-6156, 2006.
R. Fujino, Y. Ishikawa, K. Tanaka, M. Kanatsu-Shinohara, K. Tamura, H. Kogo, T. Shinohara and T. Hara. Capillary morphogenesis gene (CMG)-1 is among the genes differentially expressed in mouse male germ line stem cells and embryonic stem cells. Mol. Reprod. Dev., 73: 955-966, 2006.
R. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, H. Kogo, and T. Hara. Spermatogonial cell-mediated activation of an IkappaBzeta-independent NF-kappaB pathway in Sertoli cells induces transcription of the lipocalin-2 gene. Mol. Endocrinol., 20: 904-915, 2006.
M. Yoshie, K. Tamura, T. Hara, and H. Kogo. Expression of stathmin family genes in the murine uterus during early pregnancy. Mol. Reprod. Dev.,73: 164-172, 2006.
2005年
R. Doyonnas, J. S. Nielsen, S. Chelliah, E. Drew, T. Hara, A. Miyajima, and K. M. McNagny. Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells. Blood, 105: 4170-4178, 2005.
H. Eda, Y. Ishii, M. Obayashi, S. Harada, S. Ito, T. Fujita, M. Ikeda, S. Kusano, R. Kitamura, C. Suzuki, T. Hara, M. Watanabe, H. Satoh, K. Sugihara, and K. Yanagi. Monoclonal antibodies against regions topologically surrounding the homodimeric beta-barrel interface of Epstein-Bar virus nuclear antigen-1. Virus Research, 109: 87-94, 2005.
K. Iwatsuki, K. Tanaka, T. Kaneko, R. Kazama, S. Okamoto, Y. Nakayama, Y. Ito, M. Satake, S. Takahashi, A. Miyajima, T. Watanabe, and T. Hara. Runx1 promotes angiogenesis by downregulation of insulin-like growth factor binding protein-3. Oncogene, 24: 1129-1137, 2005.