感染制御プロジェクト｜インフルエンザ・デング熱及び肝疾患に対する予防と治療
  • 医学研ロゴ

Achievements

Achievements

2014年

  • Kyoko Tsukiyama-Kohara and MichinoriKohara. Tupaiabelangeri as an experimental animal model for viral infection.Experimental Animals. 2014 Oct 30;63(4):367-74. Epub 2014 Jul 22.
  • Chao-Kuen Lai, Vikas Saxena, Chung-Hsin Tseng, King-Song Jeng, Michinori Kohara, and Michael M. C. Lai. Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport.PLoS One. 2014; 9(6): e99022. doi: 10.1371/journal.pone.0099022
  • Tsubasa Munakata, Makoto Inada, Yuko Tokunaga, Takaji Wakita, Michinori Kohara, and Akio Nomoto. Suppression of hepatitis C virus replication by cyclin-dependent kinase inhibitors.Antiviral Research.2014 Aug;108:79-87. doi: 10.1016/j.antiviral.2014.05.011.
  • Masaaki Arai, Kyoko Tsukiyama-Kohara, Asako Takagi, Yoshimi Tobita, Kazuaki Inoue and MichinoriKohara. Resistance to cyclosporin A derives from mutations in Hepatitis C virus nonstructural proteins.Biochem Biophys Res Commun. 2014 May 23;448(1):56-62. doi: 10.1016/j.bbrc.2014.04.053.
  • Tsunamasa Watanabe, HirotoHatakeyama, Chiho Matsuda-Yasui, Yusuke Sato, Masayuki Sudoh, Asako Takagi, Yuichi Hirata, Takahiro Ohtsuki, Masaaki Arai, Kazuaki Inoue, Hideyoshi Harashima and Michinori Kohara. In vivo therapeutic potential of Dicer-hunting siRNAs targeting infectious hepatitis C virus.Scientific Reports. 2014 Apr 23;4:4750. doi: 10.1038/srep04750.
  • Yuri Kasama, Takuo Mizukami, Hideki Kusunoki, Jan Peveling-Oberhag, Yasumasa Nishito, Makoto Ozawa, Michinori Kohara Toshiaki Mizuochi, and Kyoko Tsukiyama-Kohara. B-cell-intrinsic Hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-kB signaling.PLoSONE. 9(3):e91373. doi: 10.1371/journal.pone.0091373. eCollection 2014. (2014).
  • FumihikoYasui, Michinori Kohara, Masahiro Kitabatake, Toru Nishiwaki, Hideki Fujii, Chise Tateno, Misako Yoneda, Kouichi Morita, Kouji Matsushima, Shigeo Koyasu, Chieko Kai. Phagocytic cells contribute to the antibody-mediated elimination of pulmonary-infected SARS coronavirus.Virology. 454-455:157-68. doi: 10.1016/j.virol.2014.02.005. Epub 2014 Mar 4.(2014).
  • Haru Ogiwara, FumihikoYasui, Keisuke Munekata, Asako Takagi-Kamiya, Tsubasa Munakata, Namiko Nomura, Futoshi Shibasaki, Kazuhiko Kuwahara, Nobuo Sakaguchi, Yoshihiro Sakoda, Hiroshi Kida and MichinoriKohara. Histopathological evaluation of the diversity of cells susceptible to H5N1 virulent avian influenza virus.The American Journal of Pathology. 184(1):171-83 (2014).

2013年

  • Tomoko Fujiyuki, Misako Yoneda, FumihikoYasui, Takeshi Kuraishi, Shosaku Hattori, Hyun-jeong Kwon, KeisuikeMunekata, Yuri Kiso, Hiroshi Kida, Michinori Kohara, Chieko Kai. Infection of a Japanese isolate of highly pathogenic avian influenza virus (H5N1) to macaques by aerosol exposure.PLoSONE. 8(12):e83551(2013).
  • Masaaki Arai, Yuko Tokunaga, AsakoTakagia, Yoshimi Tobita, Yuichi Hirata, Yuji Ishida, Chise Tateno, Michinori Kohara. Isolation and characterization of highly replicable hepatitis C virus genotype 1a strain HCV-RMT.PLoSONE. 8(12):e82527. (2013).
  • Takeshi Wada, Michinori Kohara and Yasuhiro Yasutomi. : DNA vaccine expressing the non-structural proteins of hepatitis C virus diminishes the expression of HCV proteins in a mouse model.Vaccine31(50):5968-74 (2013)
  • Akira Sakurai, Katuyoshi Takayama, Namiko Nomura, Tsubasa Munakata, Naoki Yamamoto, Tsuruki Tamura, Jitsuho Yamada, Masako Hashimoto, Kazuhiko Kuwahara, Masatoshi Okamatsu, Yoshihiro Sakoda, Yoshihiko Suda, Yukuharu Kobayashi, Nobuo Sakaguchi, Hiroshi Kida, Michinori Kohara and Futoshi Shibasaki. : Broad-spectrum detection of H5 subtype influenza A viruses with a new fluorescent immunochromatography system.PLoSONE. 8(11):e76753 (2013)
  • Asao Katsume, Yuko Tokunaga, Yuichi Hirata, Tsubasa Munakata, Makoto Saito, Hitohisa Hayashi, Koichi Okamoto, Yusuke Ohmori, Isamu Kusanagi, Shinya Fujiwara, Takuo Tsukuda, Yuko Aoki, Klaus Klumpp, Kyoko Tsukiyama-Kohara, Ahmed El-Gohary, Masayuki Sudoh, Michinori Kohara. A Serine Palmitoyltransferase Inhibitor Inhibits Hepatitis C Virus Replication in Human Hepatocytes.Gastroenterology.145(4):865-73 (2013).
  • Watanabe T., Sugauchi F, Tanaka Y., Matsuura M., Yatsuhashi H., Murakami S., Iijima S.,Iio E., Sugiyama M., Shimada T., Kakuni M., Kohara M., Mizokami M. Hepatitis C virus kinetics by administration of pegylated interferon-α in human and chimeric mice carrying human hepatocytes with variants of the IL28B gene.Gut62(9):1340-6 (2013)
  • Aoki J., Kowazaki Y., Ohtsuki T., Okamoto R., Ohashi K., Hayashi S., Sakamaki S., Kohara M., Kimura K. Kinetics of peripheral hepatitis B virus-specific CD8+ T cells in patients with onset of viral reactivation.J. Gastroenterology48(6):728-37 (2013)
  • Kazuya Shiogama,Ken-ichi Inada, Michinori Kohara, Hidemi Teramoto, Yasuyoshi Mizutani, Takanori Onouchi, and Yutaka Tsutsumi. Demonstration of hepatitis C virus RNA with high sensitivity in situ hybridization employing a locked nucleic acid probe in humanized liver of infected chimeric mice and in needle biopsied human liver.International Journal of Hepatology.ID249535, 7pages doi.org/10.1155/2013/249535 (2013).
  • Kyoko Tsukiyama-Kohara, Asao Katsume, Kazuhiro Kimura, Masayuki Saito, and Michinori Kohara. 4E-BP1 plays a suppressive role in differentiation of brown adipocytes from white adipose tissue.Gene Cell.18(7):602-607 (2013) DOI: 10.1111/gtc.12059
  • Shin-ichiro Nakagawa, Yuichi Hirata, Takeshi Kameyama, Yuko Tokunaga, Yasumasa Nishito, Kazuko Hirabayashi, Junichi Yano, Takahiro Ochiya, Chise Tateno, Yasuhito Tanaka, Masashi Mizokami, Kyoko Tsukiyama-Kohara, Kazuaki Inoue, Makoto Yoshiba, Akinori Takaoka and Michinori Kohara. Targeted induction of interferon-λ in humanized chimeric mouse liver abrogates hepatotropic virus infection.PLoS One.8(3):e59611 (2013).
  • Fumihiko Yasui, Masayuki Sudoh, Masaaki Arai,Michinori Kohara. Synthetic lipophilic antioxidant BO-653 suppresses HCV replication.J Med Virol.85(2):241-249 (2013).

2012年

  • Tsunamasa Watanabe, Fuminaka Sugauchi, Yasuhito Tanaka, Kentaro Matsuura,Hiroshi Yatsuhashi, Shuko Murakami, Sayuki Iijima, Etsuko Iio, Masaya Sugiyama, Takashi Shimada, Masakazu Kakuni, Michinori Kohara, Masashi Mizokami. Hepatitis C virus kinetics by administrationof pegylated interferon-α in human and chimericmice carrying human hepatocytes with variantsof the IL28B gene.Gut.(2012) in press.
  • Satoshi Sekiguchi, Kiminori Kimura, Tomoko Chiyo, Takahiro Ohtsuki, Yoshimi Tobita, Yuko Tokunaga, Fumihiko Yasui, Kyoko Tsukiyama-Kohara, Takaji Wakita, Toshiyuki Tanaka, Masayuki Miyasaka, Kyosuke Mizuno, Yukiko Hayashi, Tsunekazu Hishima, Kouji Matsushima and Michinori Kohara. Immunization with a recombinant vaccinia virus that encodes nonstructural proteins of the hepatitis C virus suppresses viral protein levels in mouse liver.PLoS One.7(12):e51656 (2012).
  • Kazuaki Inoue, Kyoko Tsukiyama-Kohara, Chiho Matsuda1, Mitsutoshi Yoneyama, Takashi Fujita, Shusuke Kuge, Makoto Yoshiba and Michinori Kohara. Impairment of interferon regulatory factor-1 3 activation by hepatitis C virus core protein basic region 1.Biochem Biophys Res Commun.428(4):494-499 (2012).
  • Leiyun Weng, Xiao Tian, Yayi Gao, Koichi Watashi, Kunitada Shimotohno, Takaji Wakita, Michinori Kohara, Tetsuya Toyoda. Different mechanisms of hepatitis C virus RNA polymerase activation by cyclophilin A and B in vitro.Biochim Biophys Acta. 1820(12):1886-1892 (2012).
  • Jun Aoki, Yuka Kowazaki, Takahiro Ohtsuki, Rumiko Okamoto, Kazuteru Ohashi, Seishu Hayashi, Hisashi Sakamaki, Michinori Kohara and Kiminori Kimura. Kinetics of Peripheral Hepatitis B Virus-specific CD8+ T Cells in Patients with Onset of Viral Reactivation.J Gastroenterology. (2012) in press.
  • Yuichi Hirata, Kazutaka Ikeda, Masayuki Sudoh, Akemi Suzuki,Yuko Tokunaga, Leiyun Weng, Masatoshi Ohta, Yoshimi Tobita, Ken Okano, Kazuhisa Ozeki, Kenichi Kawasaki, Takuo Tsukuda, Asao Katsume, Yuko Aoki, Takuya Umehara, Satoshi Sekiguchi, Tetsuya Toyoda,Kunitada Shimotohno, Tomoyoshi Soga,Masahiro Nishijima, Ryo Taguchi, and Michinori Kohara. Self-enhancement of Hepatitis C Virus Replication by Promotion of Specific Sphingolipid Biosynthesis.PLoS Pathog. 2012 Aug;8(8):e1002860. Epub 2012 Aug 16. (2012).
  • Qiang Wang, Shijian Zhang, Hongbing Jiang, Jinalan Wang, Leiyun, Weng, Yingying Mao, Satoshi Sekiguchi, Fumihiko Yasui, Michinori Kohara,Philippe Buchy, Vincent Deubel, Ke Xu, Bing Sun and Tetsuya Toyoda. PA from an H5N1 highly pathogenic avian influenza virus activatesviral transcription and replication, and induces apoptosis and interferonexpression.Virol J. 8;9:106-118 (2012).
  • Naoko Kubota, Yasutaka Inayoshi, Naoko Satoh, Takashi Fukuda, Kenta Iwai, Hiroshi Tomoda, Michinori Kohara, Kazuhiro Kataoka, Akira Shimamoto, Yasuhiro Furuichi, Akio Nomoto, Akira Naganuma and Shusuke Kuge. HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells.FEBS letter. 30;586(16):2318-2325 (2012).
  • Hideyuki Konishi, Koichi Okamoto, Yusuke Ohmori, Hitoshi Yoshino, Hiroshi Ohmori, Motooki Ashiara, Yuichi Hirata, Atsunori Ohta, Hiroshi Sakamoto, Natsuko Hada, Asao Katsume, Michinori Kohara, Kazumi Morikawa, Takuo Tsukuda, Nobuo Shimma, Graham Foster, William Alazawi, Yuko Aoki, Mikio Arisawa, and Masayuki Sudoh. An orally available, small-molecule interferon inhibits hepatitis C virus replication.Sci Comm. 2:259:1-9 (2012).
  • Leiyun Weng; Michinori Kohara; Takaji Wakita; Kunitada Shimotohno; Tetsuya Toyoda. Detergent-induced activation of the hepatitis C virus genotype 1b RNA polymerase.Gene. 496:79-87 (2012).
  • Makoto Saito, Michinori Kohara, Yuri Kasama and Kyoko Tsukiyama-Kohara. Hepatitis C virus induces overexpression of 3ß-hydroxysterol ∆24-reductase through Sp1.J Med Virol.84:733-746 (2012).
  • Yuri Kasama, Makoto Saito, Takashi Takano, Tomohiro Nishimura, Masaaki Satoh, Zhonzhi Wang, Nagla Elwy, Shinji Harada, Michinori Kohara, Kyoko Tsukiyama-Kohara. Translocase of outer mitochondrial membrane 70 induces interferonresponse and is impaired by hepatitis C virus NS3.Virus Res. 163:405-409 (2012).

2011年

  • Masaaki Satoh, Makoto Saito, Takashi Takano, Yuri Kasama, Tomohiro Nishimura, Yasumasa Nishito, Yuichi Hirata, Masaaki Arai, Masayuki Sudo, Chieko Kai, Michinori Kohara, and Kyoko Tsukiyama-Kohara. Monoclonal antibody 2-152a suppresses hepatitis C virus infection through betaine/GABA transporter-1.J Infect Dis. 204(8):1172-1180 (2011).
  • Tomoko Chiyo, Satoshi Sekiguchi, Masahiro Hayashi, Yoshimi Tobita, Yumi Kanegae, Izumu Saito, and Michinori Kohara. Conditional hepatitis C virus gene expression without induction of severe inflammatory responses through the use of a Cre-expressing recombinant adenovirus in mice.Virus Res. 160(1-2):89-97 (2011).
  • Takashi Takano, Kyoko Tsukiyama-Kohara, Masahiro Hayashi, Yuichi Hirata, Masaaki Satoh, Chise Tateno, Yukiko Hayashi, Tsunekazu Hishima, Nobuaki Funata, Masayuki Sudo, and Michinori Kohara. Augmentation of DHCR24 expressionby hepatitis C virus infection facilitates viral replication in hepatocytes.J Hepatology. 55(3):512-521 (2011)
  • Kiminori Kimura, Michinori Kohara.Frontiers of Model Animals for Human Diseases.Exp Anim. 60(2):93-100 (2011).
  • Takashi Takano, Michinori Kohara, Yuri Kasama, Tomohiro Nishimura, Makoto Saito, Chieko Kai, and Kyoko Tsukiyama-Kohara. Translocase of outer mitochondrial membrane 70 is induced by hepatitis C virus and is related to the apoptotic response.J Med Virol. 83:801-809 (2011).
  • Kiminori kimura, Satoshi Sekiguchi, Seishu Hayashi, Yukiko Hayashi, Tsunekazu Hishima, Masahito Nagaki, and Michinori Kohara. Role of interleukin-18 in intrahepatic inflammatory cell recruitment in acute liver injury.J Leukoc Biol. 89:433-442 (2011).
  • Masaaki Arai, Hidenori Suzuki, Yoshimi Tobita, Asako Takagi, Koichi Okamoto, Atsunori Ohta, Masayuki Sudoh, Kunitada Shimotohno, Michinori Kohara. Establishment of infectious HCV virion-producing cells with newly designed full-genome replicon RNA.Arch Virol. 156:295-304 (2011).
  • Kayo Yoshikawa, Aya Ogata, Chiho Matsuda, Michinori Kohara, Hideo Iba, Yukio Kitade, Yoshihito Ueno. Incorporation of biaryl units into the 5′ and 3′ ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance.Bioconjugate Chem. 22:42-49 (2011).

2010年

  • Leiyun Weng, Yuichi Hirata, Masaaki Arai, Michinori Kohara, Takaji Wakita, Koichi Watashi, Kunitada Shimotohno, Ying He, Jin Zhong, Tetsuya Toyoda. Sphingomyelin activates hepatitis C virus RNA polymerase in a genotype specific manner.J Virol.84(22):11761-11770 (2010).
  • Hideko Nuriya, Kazuaki Inoue, Takeshi Tanaka, Yukiko Hayashi, Tsunekazu Hishima, Nobuaki Funata, Kyosuke Kaji, Seishu Hayashi, Shuichi Kaneko and Michinori Kohara. Detection of hepatitis B and C viruses in almost all hepatocytes by modified PCR-based in situ hybridization.J Clin Microviol.48(11):3843-3851 (2010).
  • Yuri Kasama, Satoshi Sekiguchi, Makoto Saito, Kohsuke Tanaka, Masaaki Satoh, Kazuhiko Kuwahara, Nobuo Sakaguchi, Motohiro Takeya, Yoichi Hiasa, Michinori Kohara, and Kyoko Tsukiyama-Kohara. Persistent expression of the full genome of hepatitis C virus in B cells induces spontaneous development of B-cell lymphomasin vivo.Blood. 116(23):4926-4933 (2010).
  • Kenichi Satoh, Hiroki Takahashi, Chiho Matsuda, Takuya Umehara, Toshiyuki Tanaka, Masayuki Miyasaka, Mikio Zeniya and Michinori Kohara. Natural killer cells target HCV core proteins during the innate immune response in HCV transgenic mice.J Med Virol. 82(9):1545-1553 (2010).
  • Masaaki Satoh, Makoto Saito, Kohsuke Tanaka, Sumako Iwanaga, Salem Nagla Elwy Salem Ali, Takahiro Seki, Seiji Okada, Michinori Kohara, Shinji Harada, Chieko Kai, Kyoko Tsukiyama-Kohara. Evaluation of a recombinant measles virus expressing hepatitis C virus envelope proteins by infection of human PBL-NOD/Scid/Jak3null mouse.Comp Immunol Microbiol Infect Dis. Dec;33(6):e81-88 (2010).
  • Chen Y-Z, Liu G, Senju S, Wang Q, Irie A, Haruta M, Matsui M, Yasui F, Kohara M, and Nishimura Y. Identification of SARS-COV spike protein-derived and HLA-A2-restricted human CTL epitopes by using a new muramyl dipeptidederivative adjuvant.Int J Immunopathol Pharmacol. 23(1):165-177 (2010).
  • Yutaka Amako, Kyoko Tsukiyama-Kohara, Asao Katsume, Yuichi Hirata,Satoshi Sekiguchi, Yoshimi Tobita, Yukiko Hayashi, Tsunekazu Hishima, Nobuaki Funata, Hiromichi Yonekawa and Michinori Kohara. Pathogenesis of hepatitis C virus infection inTupaia belangeri.J Virol. 84(1):303-311 (2010).

2009年

  • Tomohiro Nishimura, Michinori Kohara, Kosuke Izumi, Yuri Kasama, Yuichi Hirata, Ying Huang, Masahiro Shuda, Hideko Nuriya, Yuko Tokunaga, Masaaki Sato, Makoto Saito, Chieko Kai and Kyoko Tsukiyama-Kohara. Hepatitis C virus impairs P53 via persistent over-expression of 3ß-hydroxysterol ∆24-reductase.J Biol Chem. 284(52):36442-36452 (2009).
  • Hirofumi Uto, Sherri O. Stuver, Katsuhiro Hayashi, Kotaro Kumagai, Fumisato Sasaki, Shuji Kanmura, Masatsugu Numata, Akihiro Moriuchi, Susumu Hasegawa, Makoto Oketani, Akio Ido, Kazunori Kusumoto, Satoru Hasuike, Kenji Nagata, Michinori Kohara, Hirohito Tsubouchi. Increased rate of death related to presence of viremia among hepatitis C virus antibody-positive subjects in a community-based cohort study.Hepatology. Aug;50(2):393-399. (2009).
  • Keigo Machida, Kyoko Tsukiyama-Kohara, Eiji Seike, Shigenobu Tóne, Yukiko Hayashi, Yuri Kasama, Masumi Shimizu, Hidemi Takahashi, Chyoji Taya, Hiromichi Yonekawa, Nobuyuki Tanaka, and Michinori Kohara. Disruption of IFN Signaling and HCV Synergistically Enhance Lymphoproliferation through Type II CD95 and Interleukins.Gastroenterology. 137(1):285-296. (2009).
  • Leiyun Weng, Jiamu Du, Jingling Zhou, Jianping Ding, Takaji Wakita, Michinori Kohara, Tetsuya Toyoda. Modification of hepatitis C virus 1b RNA polymeraseto make a highly active JFH1-type polymerase by mutationof the thumb domain.Arch Virol. 154(5):765-773. (2009).
  • Yoshihito Ueno, Yuuji Watanabe, Aya Shibata, Kayo Yoshikawa, Takashi Takano,Michinori Kohara, Yukio Kitade. Synthesis of nuclease-resistant siRNAs possessing universal overhangs.Bioorg Med Chem. 17(5):1974-1981. (2009).

2008年

  • Kei Ogata, Takahito Kashiwagi, Jun Iwahashi, Koyu Hara, Haruhito Honda, Tatsuya Ide, Ryukichi Kumashiro, Michinori Kohara, Michio Sata, and Nobuyuki Hamada. A mutational shift from domain III to II in the internal ribosome entry site of hepatitis C virus after interferon-ribavirin therapy.Arch Virol. 153(8):1575-1579. (2008).
  • Fumihiko Yasui, Chieko Kai, Masahiro Kitabatake, Shingo Inoue, Misako Yoneda, Shoji Yokochi, Ryoichi Kase, Satoshi Sekiguchi, Kouichi Morita, Tsunekazu Hishima, Hidenori Suzuki, Katsuo Karamatsu, Yasuhiro Yasutomi, Hisatoshi Shida, Minoru Kidokoro, Kyosuke Mizuno, Kouji Matsushima, Michinori Kohara. Prior immunization with SARS-CoV nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.J Immunol. 181(9):6337-6348 (2008).
  • T. Nishimura, M. Saito, T. Takano, A. Nomoto,M. Kohara, K. Tsukiyama-Kohara. Comparative aspects on the role of polypyrimidine tract-binding protein in internal initiation of hepatitis C virus and picornavirus RNAs. Compara. Immu. Microbio. Infect. Dises. 31: 435-448 (2008).
  • Naoya Sakamoto, Yoko Tanabe, Takanori Yokota, Kenichi Satoh, Yuko Sekine-Osajima, Mina Nakagawa, Yasuhiro Itsui, Megumi Tasaka, Yuki Sakurai, Chen Cheng-Hsin, Masahiko Yano, Shogo Ohkoshi, Yutaka Aoyagi, Shinya Maekawa, Nobuyuki Enomoto, Michinori Kohara, and Mamoru Watanabe. Inhibition of hepatitis C virus infection and expression in vitro and in vivo by recombinant adenovirus expressing short hairpin RNA. J. Gastroenterology Hepatology. 23(9):1437-47 (2008).
  • Sachiko Inubushi, Motoko Nagano-Fujii, Kikumi Kitayama, Motofumi Tanaka, Chunying An, Hiroshi Yokozaki, Hirohei Yamamura, Hideko Nuriya, Michinori Kohara, Kiyano Sada, and Hak Hotta. Hepatitis C virus NS5A protein interacts with and negatively regulates the non-receptor protein-tyrosine kinase Syk. J. Gen. Virology 89: 1231-1242 (2008).

2007年

  • Tsunamasa Watanabe,Takuya Umehara,Michinori Kohara. Therapeutic application of RNA interference for Hepatitis C Virus. Advanced Drug Delivery Reviews 59: 1263-1276 (2007).
  • Tsunamasa Watanabe,Takuya Umehara,Fumihiko Yasui,Shin-ichiro Nakagawa, Junichi Yano, Tadaaki Ohgi, Satoru Sonoke,Kazuaki Inoue,Makoto YoshibaandMichinori Kohara. Liver target delivery of small interfering RNA to the HCV gene by lactosylated cationic liposome. J. Hepatology 47: 744-750 (2007).
  • Totsugawa T, Yong C, Rivas-Carrillo JD, Soto-Gutierrez A, Navarro-Alvarez N, Noguchi H, Okitsu T, Westerman KA,Kohara M, Reth M, Tanaka N, Leboulch P, Kobayashi N. Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination. J. Hepatology 47: 74-82 (2007).
  • Toshie Mashiba, Keiko Udaka, Yasuko Hirachi, Yoichi Hiasa, Tomoya Miyakawa, Yoko Satta, Tsutomu Osoda, Sayo Kataoka,Michinori Koharaand Morikazu Onji. Identification of CTL epitopes in hepatitis C virus by a genome-wide computational scanning and a rational design of peptide vaccine. Immunogenetics 59: 197-209 (2007).
  • Kazuaki Inoue,Takuya Umehara, Urs T. Ruegg,Fumihiko Yasui,Tsunamasa Watanabe,Hiroshi Yasuda,Makoto Yoshiba, Jean-Maurice Dumont, Pietro Scalfaro,Michinori Kohara. Evaluation of the cyclophilin inhibitor DEBIO-025 in hepatitis C virus-infected chimeric mice in vivo. Hepatology 45: 921-928 (2007).
  • Shin-ichiro Nakagawa,Takuya Umehara,Chiho Matsuda, Shusuke Kuge, Masayuki Sudoh andMichinori Kohara. Inhibition of Hsp90 suppresses HCV replication in replicon cell lines and in chimeric mice with humanized liver. Biochem. Biophys. Res. Commun. 353: 882-888 (2007).
  • Masahiro Kitabatake, Shingo Inoue, Fumihiko Yasui, Shoji Yokochi, Masaaki Arai, Kouichi Morita, Hisatoshi Shida, Minoru Kidokoro, Fukashi Murai, Mai Quynh Le, Kouji Matsushima andMichinori Kohara. SARS-CoV spike protein recombinant vaccinia virus efficiently induces neutralizing antibodies in spite of pre-immunization with vaccinia virus. Vaccine 25: 630-637 (2007)

2006年

  • Noriyuki Kanzawa, Kazuo Nishigaki, Takaya Hayashi, Yuichi Ishii, Souichi Furukawa, Ayako Niiro,Fumihiko Yasui,Michinori Kohara, Koichi Morita, Kouji Matsushima, Mai Quynh Le, Takao Masuda, and Mari Kannagi. Augmentation of chemokine production by severe acute respiratory syndrome coronavirus 3a/X1 and 7a/X4 proteins through NFkB activation. FEBS letter 580, 6807-6812 (2006).
  • Tsunamasa WatanabeandMichinori Kohara: Intracellular diced long-dsRNA has enhanced efficacy for silencing HCV RNA and overcome variation in viral genotype. RNAi Therapeutics, 2006 : Editors: Hiroshi Takaku and Naoki Yamamoto
  • Masahiko Kaito, Shozo Watanabe, Hideki Tanaka, Naoki Fujita, Masayoshi Konishi, Motoh Iwase, Yoshinao Kobayashi, Esteban Cesar Gabazza, Yoshihiko Adachi,Kyoko-Tsukiyama-Kohara, andMichinori Kohara. Morphological identification of hepatitis C virus E1 and E2 envelope glycoproteins on the virion surface using immunogold electron microscopy. International j. Mol. Med. 18: 673-678 (2006).
  • Masahiko Kaito, Esteban C Gabazza, Naoki Fujita, Hideaki Tanaka, Shozo Watanabe,Michinori Kohara. Immune complex of hepatitis C virus particles detected by immunogold electron microscopy. J. Gastroenterology 41:807-809 (2006).
  • Masahiko Kaito • Hiroyoshi Ohba • Joe ChibaMichinori Kohara• Hideaki Tanaka • Naoki FujitaEsteban Cesar Gabazza • Shozo WatanabeMasayoshi Konishi • Yukihiko Adachi. The ultrastructural morphology of native hepatitis B virus. Med Mol Morphol 39:136–145 (2006)
  • Miki A, Narushima M, Okitsu T, Takeno Y, Soto-Gutierrez A, Rivas-Carrillo JD, Navarro-Alvarez N, Chen Y, Tanaka K, Noguchi H, Matsumoto S,Kohara M,Lakey JR, Kobayashi E, Tanaka N, Kobayashi N. Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts.Cell Transplant. 2006;15(4):325-34.
  • Yuichiro Nakatsu, Makoto Takeda, Minoru Kidokoro,Michinori Koharaand Yusuke Yanagi. Reverse genetics system for measles virus driven by vaccinia virus Lister vaccine strain. Journal of Virological Methods 137:152-155 (2006).
  • Teruhiko Suzuki, Yoshiakira Kanai, Takahiko Hara, Junko Sasaki, Takehiko Sasaki,Michinori Kohara, Tomohiko Maehama, Choji Taya, Hiroshi Shitara, Hiromichi Yonekawa, Michael A. Frohman, Takeaki Yokozeki & Yasunori Kanaho. Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development. Mol. Cell. Biology 26(16):6149-6156 (2006).
  • Kyoko Murakami, Koji Ishii, Yousuke Ishihara, Sayaka Yoshizaki, Keiko Tanaka, Yasufumi Gotoh, Hideki Aizaki,Michinori Kohara, Hiroshi Yoshioka, Yuichi Mori, Noboru Manabe, Ikuo Shoji, Tetsutaro Sata, Ralf Bartenschlager, Yoshiharu Matsuura, Tatsuo Miyamura and Tetsuro Suzuki. Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b. Virology 351:381-392 (2006).
  • Hironori Nishitsuji,Michinori Kohara, Mari Kannagi, and Takao Masuda. Effective Suppression of Human Immunodeficiency Virus Type 1 through Combination of Short- or Long-hairpin RNAs Targeting Essential Sequences for Retroviral Integration. J. Virology 80(15):7658-66 (2006).
  • Kaito M, Ishida S, Tanaka H, Horiike S, Fujita N, Adachi Y,Kohara M, Konishi M, Watanabe S. Morphology of hepatitis C and hepatitis B virus particles as detected by immunogold electron microscopy. Med Mol Morphol. 39(2):63-71 (2006).
  • Yoshiyuki Tanaka, Junko Kato,Michinori Kohara, Mark S. Galinski. Antiviral effects of glycosylation and glucose trimming inhibitors on human parainfluenza virus type 3. Antiviral Res. 2006 Oct;72(1):1-9.
  • Takuya Umehara, Masayuki Sudoh,Fumihiko Yasui,Chiho Matsuda, Yukiko Hayashi, andMichinori Kohara. Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model. Biochem. Biophys. Res. Commun. 346, 67-73 (2006).
  • Tsunamasa Watanabe, Masayuki Sudoh, Makoto Miyagishi, Masaaki Arai,Kazuaki Inoue, Kazunari Taira,Makoto Yoshiba,Michinori Kohara. Targeting the Hepatitis C Virus genome with RNA interference using highly effective and non-toxic long double-stranded RNA. Gene Theraphy 13: 883-892 (2006)
  • Hirofumi Uto, Katsuhiro Hayashi, Kazunori Kusumoto, Satoru Hasuike, Kenji Nagata, Mayumi Kodama, Akio Ido,Michinori Kohara, Sherri O. Stuver, Hirohito Tsubouchi. Spontaneous elimination of hepatitis C virus RNA in individuals with persistent infection in a hyperendemic area of Japan. Hepatology Research 34: 28-34 (2006)

2005年

  • Hideo Akashi, Makoto Miyagishi,Tsunamasa Watanabe, Takanori Yokota,Michinori Kohara, and Kazunari Taira. Escape from the interferon response associated with RNA interference using vectors that encode long modified hairpin-RNA. Mol. BioSystems 1(5-6):382-390 (2005).
  • Kanjiro Miyata, Yoshinori Kakizawa, Nobuhiro Nishiyama, Yuichi Yamasaki,Tsunamasa Watanabe,Michinori Kohara, and Kazunori Kataoka. Freeze-dried formulations for in vivo gene delivery of PEGylated polyplex micelles with disulfide crosslinked cores to the liver. Journal of Controlled Release 109: 15-23 (2005)
  • Hiroshi Sakamoto, Koichi Okamoto, Masahiro Aoki, Hideyuki Kato, Asao Katsume, Atsunori Ohta, Takuo Tsukuda, Nobuo Shimma, YukoAoki, Mikio Arisawa,Michinori Kohara& Masayuki Sudoh. Host sphingolipid biosynthesis as a target for hepatitis C virus therapy. Nat. Chem. Biol. 1: 333-337 (2005).
  • Kazuhiro Hayashida, Akito Daiba, Akito Sakai, Takeshi Tanaka, Kyosuke Kaji, Niro Inaba, Satoshi Ando, Naoki Kajiyama, Hiroshi Terasaki, Aki Abe, Masanori Ogasawara,Michinori Kohara, Mine Harada, Takeshi Okanoue, Satoru Ito, and Shuichi Kaneko. Pre-treatment Prediction of Interferon-a efficacy in chronic hepatitis C patients. Clinical Gastroenterology and Hepatology 3: 1253-1259 (2005).
  • Hayashi K, Hasuike S, Kusumoto K, Ido A, Uto H, Kenji N,Kohara M, Stuver SO, Tsubouchi H. Usefulness of a new immuno-radiometric assay to detect hepatitis C core antigen in a community-based population. J. Viral. Hepat. 12(1):106-10 (2005).
  • Kazuhiro Higashi,Kyoko Tsukiyama-Kohara, Takeshi Tanaka, Eiji Tanaka, Kendoh Kiyosawa andMichinori Kohara. Characterization of hypervariable region in hepatitis C virus envelope protein during acute and chronic infection. Arch. Virology 150(5):883-98 (2005).
  • Takuya Umehara, Kotaro Fukuda, Fumiko Nishikawa,Michinori Kohara,Tsunemi Hasegawa and Satoshi Nishikawa. Rational design of dual-functional aptamers that inhibit protease and helicase activities of HCV NS3. J. Biochemistry 137: 339-347 (2005).