− この都医学研セミナーは終了しました。 −
大阪医科大学生命科学講座 薬理学教室 講師
|世話人||宮岡 佑一郎 （生体分子先端研究分野 再生医療プロジェクトリーダー）|
Naive and primed pluripotent stem cells (PSCs) contribute to all three germ layers, but they exhibit differences in cell morphology, gene expression programs, and epigenetic modifications, such as the X chromosome inactivation status. Naive female PSCs have two transcriptionaly active X chromosomes (XaXa) and primed PSCs have one active and one inactive X chromosomes (XaXi). Mouse embryonic stem cells (ESCs) and induced (i)PSCs represent naive pluripotency, while mouse epiblast stem cells (EpiSCs) and most human (h)PSCs represent primed pluripotency. Recently, many attempts were made to use chemical inhibitors and cytokines to convert primed hPSCs to naive hPSCs. We previously reported that culture conditions affect X chromosome inactivation status of female hiPSCs. Female hiPSCs derived on LIF-expressing feeder cells are XaXa, but hiPSCs derived on mouse embryonic fibroblasts are XaXi inherited from donor somatic cells. These results led us to hypothesize that common PSC cell-culture media contain factors that affect epigenetic status and, probably, functionality of PSCs. In this seminar, I will discuss our attempts to identify the factors and how important to manage components in PSC culture medium. I will also talk about our efforts to model diseases using hiPSCs at Osaka Medical College where I have started my work since last July.